JUL 02, 2020 10:00 AM PDT

Hands-on Tutorial: Workflow Advances for Whole Cell Correlative Cryo-Microscopy

Sponsored by: Leica Microsystems, Alveole
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Professor of Biochemistry; Affiliate, Morgridge Institute for Research University of Wisconsin-Madison (USA)
    Biography
      Liz received her Ph.D. in Chemistry from Emory University. She engineered elastin-mimetic materials that are used for drug delivery and tissue engineering applications. She was a postdoctoral research associate in materials science at the University of Southern California. She was a postdoctoral scholar with Professor Grant Jensen at Caltech where she developed cryo-ET technologies and used cryo-ET to study HIV-1 maturation. She joined Emory University as an Assistant Professor in 2008 and was promoted to Associate Professor in 2016. She moved to the University of Wisconsin, Madison as a full Professor in 2018. Her research program focuses on the development and use of cryo-EM and correlative light and electron microscopy (CLEM) imaging technologies to determine the native-state structures of several bacterial species, bacteriophages, HIV-1, respiratory syncytial virus (RSV), measles virus (MeV), and other host-pathogen systems.
    • Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)
      Biography
        Jae completed her Ph.D. under the mentorship of Dr. Esther Bullitt at Boston University. She studied the ultrastructure and complexity of infectious extracellular vesicles released from cells infected by non-enveloped positive-sense RNA viruses using cryo-ET and mass spectrometry. After graduation, Jae joined Dr. Wright's group at UW-Madison and has focused on development of high-precision cryo-CLEM with flexible workflows and an easy-to-use software toolkit. She also works on cryo-EM sample optimization through micro-patterning and improvement of freezing conditions for cell-virus and cell-bacteria interactions, VLPs for vaccine development, and purified viral glycoproteins.
      • Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)
        Biography
          Bryan completed his Ph.D. under the mentorship of Dr. Paul Ahlquist at UW-Madison. He studied the organization and function of viral proteins essential for assembly of positive-strand RNA virus membrane-associated RNA replication complexes. Bryan is a postdoctoral research associate in Dr. Wright's lab at the UW-Madison. His research interests center around using cryo-ET to study viral structure and assembly in multiple systems including RSV, HIV-1, and Ty1 LTR-retrotransposon.
        • Graduate Student, Wright Laboratory University of Wisconsin-Madison (USA)
          Biography
            B.S. in Biochemistry and Biophysics, Oregon State University (USA)

            Joseph completed his undergraduate studies at Oregon State University in Biochemistry & Biophysics, conducting molecular biology research under Dr. Hong Moulton. He is currently a 2nd year physical chemistry Ph.D. student in Dr. Wright's lab at UW-Madison. His research interests involve using CLEM and cryo-ET to answer cell function and dysfunction questions in neuroscience. He uses both neuron-like cell lines and primary neuron cultures in his research.

          Abstract
          DATE:  July 2, 2020
          TIME:   10:00am PT, 1:00pm ET
           
          Correlative light and electron microscopy (CLEM) is used to accelerate biological discoveries. CLEM bridges between different microscopes and imaging modalities to study systems in 4D, effectively spanning gaps between cell and structural biology. CLEM technologies are rapidly advancing in order to improve biological understanding, experimental reproducibility, and automation. In this webinar, we will expand upon the basics of the technologies that were presented in the first webinar. We will describe successful step-by-step procedures for cell care, patterning substrates, culturing cells on substrates, and cryo-CLEM imaging. We will highlight validation steps and timing associated with stages throughout the process. We will also provide examples of biological systems that have been studied using these workflows.
           
          Learning Objectives:
          • Identify materials and instrumentation necessary for cryo-CLEM.
          • Incorporate advanced micro-patterning methods into cryo-CLEM pipelines.
          • Understand and use workflows for grid preparation, cell culture, and cryo-CLEM imaging.
           
          Webinars will be available for unlimited on-demand viewing after live event.
           
          LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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