Name: Aconitase Enzyme Activity Microplate Assay Kit
Availability: InStock

Aconitase Enzyme Activity Microplate Assay Kit | Creative BioMart

Aconitase Enzyme Activity Microplate Assay Kit is a simple, reproducible, and sensitive tool for assaying aconitase from tissue homogenates or cell lysates. Unlike other assays this is not a coupled reaction and therefore only aconitase activity is required and measured. Aconitase catalyzes an equilibrium between aconitate, cis-aconitate and iso-citrate. These reactions are monitored by measuring the increase in absorbance at 240 nm associated with the formation of the cis-aconitate which has an extinction coefficient of 2.2 OD/mM per well. Therefore the rate of cis-aconitate production is proportional to aconitase activity.

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