The TR-FRET ER alpha Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) alpha. The kit uses a human ER alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule ER ligand (ES2) in a homogeneous mix-and-read assay format.
The Lanthascreen ER alpha Competitive Binding Assay is:
? Complete & ready-to-use out of the box?all reagents included, just add your test compounds
? High throughput compatible?TR-FRET assays in a 384-well plate
? Convenient?components available in bulk sizes for larger screening needs