Name: Peroxidase Activity Colorimetric/Fluorometric Assay Kit
Availability: InStock

Peroxidase Activity Colorimetric/Fluorometric Assay Kit | Creative BioMart

Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR + electron donor (2 e-) + 2H+ _ ROH + ROH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.

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Creative BioMart is a professional biotechnology supplier focused on the development and production of recombinant protein products. Over the years, we have grown exponentially to offer over 100,000 protein products, including recombinant protein, native proteins, cell/tissue lysates, chromatography, lectins, and detection kits. In response to customers'...