Creative Biolabs is the leading custom service provider with extensive experience in various antibody production and engineering fields.
T-cells are functionally divided into helper T-cells, which are CD4+ CD8- (""T4""), and cytotoxic T-cells, which are CD8+ CD4- (""T8"").
T cells can also be divided based on differentiation status into 4 subsets:
1. Na?ve (""Tn "", containing Tscm), which are CD27+ CCR7+ CD45RO
2. Central-memory (""Tcm""), which are CD27+ CCR7+ CD45RO+
3. Effector-memory (""Tem""), which are CD27- CCR7- CD45RO+
4. Effector (""Teff""), which are CD27- CCR7- CD45RO-
Human PBMC are separated into 4 subsets using antibodies and magnetic beads. The subsets were: CD4+CD8- T cells (T4), CD8+CD4- T cells (T8), CD4+ central memory T cells (Tcm) and CD4+ effector-memory T cells (Tem). Immediately after isolation, the subsets and the PBMC were analyzed by flow
cytometry for the composition of the subsets.
Creative Biolabs is the leading custom service provider with extensive experience in various antibody production and engineering fields.