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    Genetic variant

    Genetic variants define a gene that has encountered a permanent change in its DNA sequence. Some, but not all, genetic variants result in a disease. Genetic variants can be inherited or can develop overtime.

    Webinars

    • NOV 10, 2021 | 7:30 AM
      Panel Presentation: Overcoming Challenges of Copy Number Variant Interpretation with QCI Interpret and the ACMG Guidelines for CNVs
      Panel Presentation: Overcoming Challenges of Copy Number Variant...
      Copy number variant (CNV) analysis has been part of clinical diagnostics for almost two decades. With the evolution of genomic technologies for CNV detection, we have learned that CNVs can r...
      Speaker: Dan Richards, PhD , Martin Jones, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2021
      Sponsored By: QIAGEN
      2
      OCT 28, 2021 | 9:00 AM
      RT-LAMP: Best practices and tips to accelerate detection of viral pathogens, including SARS-CoV-2
      RT-LAMP: Best practices and tips to accelerate detection of viral...
      Date: October 28, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Loop-mediated isothermal amplification (LAMP) uses a stand-displacing DNA polymerase and four to six primers to rapidly amplify DNA a...
      Speaker: Ingrida Vendelė, PhD , Leann Buhrow, PhD
      Sponsored By: Thermo Fisher Scientific
      11
      OCT 27, 2021 | 10:00 AM
      A CRISPR powered NGS library prep to improve viral, bacterial and host transcript detection in shotgun metagenomic sequencing
      A CRISPR powered NGS library prep to improve viral, bacterial...
      Date: October 27, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The COVID-19 pandemic has brought global awareness to the dangers of emerging pathogens to human health and welfare. Common molecular...
      Speaker: Jon Armstrong, MSc
      Sponsored By: Jumpcode Genomics
      6 2
      OCT 26, 2021 | 9:00 AM
      Hemoglobinopathy Testing: What are the Key Points a Lab Should Consider?
      Hemoglobinopathy Testing: What are the Key Points a Lab Should...
      Date: October 26, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Capillary Electrophoresis plays an important role in the diagnosis of hemoglobinopathy carriers and patients. The general flow is the...
      Speaker: Dr. Cornelis Harteveld
      Sponsored By: Sebia, Inc.
      19
      OCT 14, 2021 | 7:00 AM
      Heterologous Protein Engineering and characterization of native regulatory elements  in E. coli using Massively Parallel CRISPR Editing
      Heterologous Protein Engineering and characterization of native...
      Date: October 14, 2021 Time: 7:00am (PDT), 10:00am (EDT) Heterologous protein expression in model organisms has many applications, including protein engineering, production of industrial enz...
      Speaker: Tyson Shepherd, PhD
      Sponsored By: Inscripta
      22
      OCT 13, 2021 | 3:00 PM
      Accelerating SARS-CoV-2 Genomic Surveillance with High-throughput NGS
      Accelerating SARS-CoV-2 Genomic Surveillance with High-throughput...
      Since late 2020, several prominent SARS-CoV-2 variants of concern have emerged, harboring specific mutations which increase viral transmissibility Delta variant) and which appear to reduce t...
      Speaker: James Caffrey, PhD, MBA
      Presented at: Coronavirus Series
      Sponsored By: QIAGEN
      1
      OCT 13, 2021 | 1:30 PM
      SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping​
      SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping​
      This presentation “SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping” will focus on strategies for the testing and detection of SARS-CoV-2, including multiplexe...
      Speaker: Manohar R. Furtado, PhD
      Presented at: Coronavirus Series
      Sponsored By: Thermo Fisher Scientific
      14
      OCT 13, 2021 | 12:00 AM
      SARS-CoV-2 Evolution: Addition by Subtraction
      SARS-CoV-2 Evolution: Addition by Subtraction
      SARS-CoV-2 is an emergent virus. Specifically how it will adapt to human hosts and populations with growing immunity is unknown. Coronaviruses acquire substitutions more slowly than other RN...
      Speaker: Kevin R. McCarthy, PhD
      Presented at: Coronavirus Series
      0
      OCT 12, 2021 | 8:00 AM
      The growing concern of off-target effects: How to measure and minimize off-target effects in your genome editing experiments.
      The growing concern of off-target effects: How to measure and...
      Date: October 12, 2021 Time: 8:00am (PDT), 11:00am (EDT) CRISPR-Cas9 is a nuclease-based genome editing system which has seen exponential growth in adoption with broad applications from basi...
      Speaker: Pandhita Pochanard
      Sponsored By: Thermo Fisher Scientific/Gibco
      30
      OCT 07, 2021 | 1:30 PM
      Harnessing Insight from Real-world Oncology Cases and QIAGEN Knowledge to Annotate Variants and Assess Prevalence: Introducing a New Somatic Database-Our Best Kept Secret
      Harnessing Insight from Real-world Oncology Cases and QIAGEN Knowledge...
      Competing to offer a comprehensive genomic profiling service for solid or hematology oncology tumor samples is challenging, especially as panels increase in size and complexity. QIAGEN Clini...
      Speaker: Beate Litzenburger, Ph.D.
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      Sponsored By: QIAGEN
      0
      OCT 07, 2021 | 12:00 PM
      Panel Presentation: Oncology Variant Interpretation Just Got More Precise
      Panel Presentation: Oncology Variant Interpretation Just Got More...
      Across oncology applications, from research to molecular testing and pharmaceutical development, the ability to identify potentially actionable genetic alterations and exploit the molecular...
      Speaker: Beate Litzenburger, Ph.D. , Sheryl Elkin, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      Sponsored By: QIAGEN
      2
      OCT 06, 2021 | 11:55 PM
      Using Networks to Understand Cancer Risk and Therapies
      Using Networks to Understand Cancer Risk and Therapies
      One of the central tenants of biology is that our genetics—our genotype—influences the physical characteristics we manifest—our phenotype. But with more than 25,000 human g...
      Speaker: John Quackenbush, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      1
      OCT 06, 2021 | 11:55 PM
      Precision Care and Health Disparities in Prostate Cancer
      Precision Care and Health Disparities in Prostate Cancer
      Data suggests that genetics comprises 42-58% of the prostate cancer risk. GWAS has found about 43% of the risk SNPs in Blacks and European Americans. Most of the SNPs that have been discover...
      Speaker: Adam B. Murphy, MD, MBA, MSCI
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      1
      OCT 06, 2021 | 6:00 AM
      COSMIC: Describing Millions of Somatic Mutations at High Resolution Across Every Form of Cancer Underpins Precision Oncology Research
      COSMIC: Describing Millions of Somatic Mutations at High Resolution...
      COSMIC is the world’s largest and most comprehensive somatic mutations resource. COSMIC’s contents are manually curated by PhD-level experts from high-quality scientific literatu...
      Speaker: Simon Forbes, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      Sponsored By: QIAGEN
      1
      SEP 09, 2021 | 7:00 AM
      Maximize your variant knowledge using the Human Gene Mutation Database (HGMD®) Professional
      Maximize your variant knowledge using the Human Gene Mutation...
      Date: September 9, 2021 Time: 7:00am (PDT), 10:00am (EDT) Founded and maintained by the Institute of Medical Genetics at Cardiff University in 1996, HGMD attempts to collate all known, publi...
      Speaker: Dr. Ruth Burton
      Sponsored By: QIAGEN
      3
      SEP 08, 2021 | 12:00 AM
      High-precision Microbial Diagnostics From Sequencing Data
      High-precision Microbial Diagnostics From Sequencing Data
      Genetic Molecular Surveillance is a key component of a modern public health toolkit. This talk will detail the molecular microbial make up of urban environments. It will discuss how these en...
      Speaker: David C. Danko, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      3
      AUG 25, 2021 | 7:00 AM
      Automation-aided targeted genotyping with Allegro for maize breeding: a case study of QTL analysis with MAGIC mapping populations
      Automation-aided targeted genotyping with Allegro for maize breeding:...
      Date: August 25, 2021 Time: 7:00am PDT DreamPrep NGS is a complete solution that combines the Fluent® Automation Workstation, the Infinite® F Nano+ plate reader and Tecan Genomics&rsq...
      Speaker: Dr. Davide Scaglione , Leonardo Caproni, PhD
      Sponsored By: Tecan
      4
      AUG 25, 2021 | 12:00 AM
      Mechanistic Insight into Tandem Repeat Expansions in Amyotrophic Lateral Sclerosis
      Mechanistic Insight into Tandem Repeat Expansions in Amyotrophic...
      Over 40 tandem repeats undergo expansion events that lead to neurological disease. This number is likely an underestimate as many repeats are difficult to amplify using existing short read s...
      Speaker: Paul Valdmanis, PhD
      Presented at: Neuroscience Virtual Event Series 2021
      Sponsored By: PacBio
      1
      JUL 29, 2021 | 9:00 AM
      Expanded Carrier Screening Research in the Genomic Era
      Expanded Carrier Screening Research in the Genomic Era
      Date: July 29, 2021 Time: 1:00am (PDT), 4:00am (EDT) Traditionally limited to a small number of phenotypes and mutations suggested to specific risk groups, the emerging field of genomics i...
      Speaker: Dr. Doron Behar, MD, PhD
      Sponsored By: Thermo Fisher Scientific
      7
      JUL 22, 2021 | 12:45 PM
      An Engineered AsCas12a nuclease facilitates the rapid generation of therapeutic cell medicines
      An Engineered AsCas12a nuclease facilitates the rapid generation...
      Though AsCas12a fills a crucial gap in the current genome editing toolbox, it exhibits relatively poor editing efficiency, restricting its overall utility. In collaboration with Integrated D...
      Speaker: John Zuris, PhD
      Presented at: GenScript Gene & Cell Engineering Virtual Summit
      3
      JUL 22, 2021 | 12:00 PM
      Building More Useful CRISPR-Cas Technologies
      Building More Useful CRISPR-Cas Technologies
      Learning Objectives: 1. Understand engineered CRISPR-Cas variants overcome natural limitations of wild-type enzymes 2. Understand the genome is now nearly completely editable 3. Understand m...
      Speaker: Ben Kleinstiver, PhD
      Presented at: GenScript Gene & Cell Engineering Virtual Summit
      2
      JUL 01, 2021 | 8:00 AM
      Quality control advancements in therapeutic genome editing and biomanufacturing
      Quality control advancements in therapeutic genome editing and...
      Date: July 1, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) What’s driving precision-medicine innovations? New advances in gene and cell therapy manufacturing, along with more...
      Speaker: Imen Mestiri, PhD
      Sponsored By: Genomic Vision
      9
      JUN 30, 2021 | 7:00 AM
      Optical Genome Mapping: Unrivaled Structural Variant Detection for Cancer Research and Precision Medicine
      Optical Genome Mapping: Unrivaled Structural Variant Detection...
      Date: June 30, 2021 Time: 7:00am (PDT), 8:00am (EDT) Join us to discover how Optical genome mapping (OGM) mapping can help you in the characterization of complex samples. OGM is a non-sequen...
      Speaker: Yannick Delpu, PhD , Darren "Ben" Finlay, PhD
      Sponsored By: Bionano
      8
      JUN 29, 2021 | 10:00 AM
      Approaches for Rapid Genomic Surveillance to Identify SARS-CoV-2 Variants
      Approaches for Rapid Genomic Surveillance to Identify SARS-CoV-2...
      Date: June 29, 2021 Time: 10:00am (PDT), 1:00pm (EDT) SARS-CoV-2 continues to evolve and surveillance of variants is a necessity. Routine analysis of genetic sequence data allows the identif...
      Speaker: Donna Wolk, MHA, PhD, D(ABMM)
      Sponsored By: Diasorin
      16
    NOV 10, 2021 | 7:30 AM
    Panel Presentation: Overcoming Challenges of Copy Number Variant Interpretation with QCI Interpret and the ACMG Guidelines for CNVs
    Panel Presentation: Overcoming Challenges of Copy Number Variant...
    Copy number variant (CNV) analysis has been part of clinical diagnostics for almost two decades. With the evolution of genomic technologies for CNV detection, we have learned that CNVs can r...
    Speaker: Dan Richards, PhD , Martin Jones, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2021
    Sponsored By: QIAGEN
    2
    OCT 28, 2021 | 9:00 AM
    RT-LAMP: Best practices and tips to accelerate detection of viral pathogens, including SARS-CoV-2
    RT-LAMP: Best practices and tips to accelerate detection of viral...
    Date: October 28, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Loop-mediated isothermal amplification (LAMP) uses a stand-displacing DNA polymerase and four to six primers to rapidly amplify DNA a...
    Speaker: Ingrida Vendelė, PhD , Leann Buhrow, PhD
    Sponsored By: Thermo Fisher Scientific
    11
    OCT 27, 2021 | 10:00 AM
    A CRISPR powered NGS library prep to improve viral, bacterial and host transcript detection in shotgun metagenomic sequencing
    A CRISPR powered NGS library prep to improve viral, bacterial...
    Date: October 27, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The COVID-19 pandemic has brought global awareness to the dangers of emerging pathogens to human health and welfare. Common molecular...
    Speaker: Jon Armstrong, MSc
    Sponsored By: Jumpcode Genomics
    6 2
    OCT 26, 2021 | 9:00 AM
    Hemoglobinopathy Testing: What are the Key Points a Lab Should Consider?
    Hemoglobinopathy Testing: What are the Key Points a Lab Should...
    Date: October 26, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Capillary Electrophoresis plays an important role in the diagnosis of hemoglobinopathy carriers and patients. The general flow is the...
    Speaker: Dr. Cornelis Harteveld
    Sponsored By: Sebia, Inc.
    19
    OCT 14, 2021 | 7:00 AM
    Heterologous Protein Engineering and characterization of native regulatory elements  in E. coli using Massively Parallel CRISPR Editing
    Heterologous Protein Engineering and characterization of native...
    Date: October 14, 2021 Time: 7:00am (PDT), 10:00am (EDT) Heterologous protein expression in model organisms has many applications, including protein engineering, production of industrial enz...
    Speaker: Tyson Shepherd, PhD
    Sponsored By: Inscripta
    22
     
     
  • OCT 13, 2021 | 3:00 PM
    Accelerating SARS-CoV-2 Genomic Surveillance with High-throughput NGS
    Accelerating SARS-CoV-2 Genomic Surveillance with High-throughput...
    Since late 2020, several prominent SARS-CoV-2 variants of concern have emerged, harboring specific mutations which increase viral transmissibility Delta variant) and which appear to reduce t...
    Speaker: James Caffrey, PhD, MBA
    Presented at: Coronavirus Series
    Sponsored By: QIAGEN
    1
    OCT 13, 2021 | 1:30 PM
    SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping​
    SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping​
    This presentation “SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping” will focus on strategies for the testing and detection of SARS-CoV-2, including multiplexe...
    Speaker: Manohar R. Furtado, PhD
    Presented at: Coronavirus Series
    Sponsored By: Thermo Fisher Scientific
    14
    OCT 13, 2021 | 12:00 AM
    SARS-CoV-2 Evolution: Addition by Subtraction
    SARS-CoV-2 Evolution: Addition by Subtraction
    SARS-CoV-2 is an emergent virus. Specifically how it will adapt to human hosts and populations with growing immunity is unknown. Coronaviruses acquire substitutions more slowly than other RN...
    Speaker: Kevin R. McCarthy, PhD
    Presented at: Coronavirus Series
    0
    OCT 12, 2021 | 8:00 AM
    The growing concern of off-target effects: How to measure and minimize off-target effects in your genome editing experiments.
    The growing concern of off-target effects: How to measure and...
    Date: October 12, 2021 Time: 8:00am (PDT), 11:00am (EDT) CRISPR-Cas9 is a nuclease-based genome editing system which has seen exponential growth in adoption with broad applications from basi...
    Speaker: Pandhita Pochanard
    Sponsored By: Thermo Fisher Scientific/Gibco
    30
    OCT 07, 2021 | 1:30 PM
    Harnessing Insight from Real-world Oncology Cases and QIAGEN Knowledge to Annotate Variants and Assess Prevalence: Introducing a New Somatic Database-Our Best Kept Secret
    Harnessing Insight from Real-world Oncology Cases and QIAGEN Knowledge...
    Competing to offer a comprehensive genomic profiling service for solid or hematology oncology tumor samples is challenging, especially as panels increase in size and complexity. QIAGEN Clini...
    Speaker: Beate Litzenburger, Ph.D.
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    Sponsored By: QIAGEN
    0
     
     
  • OCT 07, 2021 | 12:00 PM
    Panel Presentation: Oncology Variant Interpretation Just Got More Precise
    Panel Presentation: Oncology Variant Interpretation Just Got More...
    Across oncology applications, from research to molecular testing and pharmaceutical development, the ability to identify potentially actionable genetic alterations and exploit the molecular...
    Speaker: Beate Litzenburger, Ph.D. , Sheryl Elkin, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    Sponsored By: QIAGEN
    2
    OCT 06, 2021 | 11:55 PM
    Using Networks to Understand Cancer Risk and Therapies
    Using Networks to Understand Cancer Risk and Therapies
    One of the central tenants of biology is that our genetics—our genotype—influences the physical characteristics we manifest—our phenotype. But with more than 25,000 human g...
    Speaker: John Quackenbush, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    1
    OCT 06, 2021 | 11:55 PM
    Precision Care and Health Disparities in Prostate Cancer
    Precision Care and Health Disparities in Prostate Cancer
    Data suggests that genetics comprises 42-58% of the prostate cancer risk. GWAS has found about 43% of the risk SNPs in Blacks and European Americans. Most of the SNPs that have been discover...
    Speaker: Adam B. Murphy, MD, MBA, MSCI
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    1
    OCT 06, 2021 | 6:00 AM
    COSMIC: Describing Millions of Somatic Mutations at High Resolution Across Every Form of Cancer Underpins Precision Oncology Research
    COSMIC: Describing Millions of Somatic Mutations at High Resolution...
    COSMIC is the world’s largest and most comprehensive somatic mutations resource. COSMIC’s contents are manually curated by PhD-level experts from high-quality scientific literatu...
    Speaker: Simon Forbes, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    Sponsored By: QIAGEN
    1
    SEP 09, 2021 | 7:00 AM
    Maximize your variant knowledge using the Human Gene Mutation Database (HGMD®) Professional
    Maximize your variant knowledge using the Human Gene Mutation...
    Date: September 9, 2021 Time: 7:00am (PDT), 10:00am (EDT) Founded and maintained by the Institute of Medical Genetics at Cardiff University in 1996, HGMD attempts to collate all known, publi...
    Speaker: Dr. Ruth Burton
    Sponsored By: QIAGEN
    3
     
     
  • SEP 08, 2021 | 12:00 AM
    High-precision Microbial Diagnostics From Sequencing Data
    High-precision Microbial Diagnostics From Sequencing Data
    Genetic Molecular Surveillance is a key component of a modern public health toolkit. This talk will detail the molecular microbial make up of urban environments. It will discuss how these en...
    Speaker: David C. Danko, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    3
    AUG 25, 2021 | 7:00 AM
    Automation-aided targeted genotyping with Allegro for maize breeding: a case study of QTL analysis with MAGIC mapping populations
    Automation-aided targeted genotyping with Allegro for maize breeding:...
    Date: August 25, 2021 Time: 7:00am PDT DreamPrep NGS is a complete solution that combines the Fluent® Automation Workstation, the Infinite® F Nano+ plate reader and Tecan Genomics&rsq...
    Speaker: Dr. Davide Scaglione , Leonardo Caproni, PhD
    Sponsored By: Tecan
    4
    AUG 25, 2021 | 12:00 AM
    Mechanistic Insight into Tandem Repeat Expansions in Amyotrophic Lateral Sclerosis
    Mechanistic Insight into Tandem Repeat Expansions in Amyotrophic...
    Over 40 tandem repeats undergo expansion events that lead to neurological disease. This number is likely an underestimate as many repeats are difficult to amplify using existing short read s...
    Speaker: Paul Valdmanis, PhD
    Presented at: Neuroscience Virtual Event Series 2021
    Sponsored By: PacBio
    1
    JUL 29, 2021 | 9:00 AM
    Expanded Carrier Screening Research in the Genomic Era
    Expanded Carrier Screening Research in the Genomic Era
    Date: July 29, 2021 Time: 1:00am (PDT), 4:00am (EDT) Traditionally limited to a small number of phenotypes and mutations suggested to specific risk groups, the emerging field of genomics i...
    Speaker: Dr. Doron Behar, MD, PhD
    Sponsored By: Thermo Fisher Scientific
    7
    JUL 22, 2021 | 12:45 PM
    An Engineered AsCas12a nuclease facilitates the rapid generation of therapeutic cell medicines
    An Engineered AsCas12a nuclease facilitates the rapid generation...
    Though AsCas12a fills a crucial gap in the current genome editing toolbox, it exhibits relatively poor editing efficiency, restricting its overall utility. In collaboration with Integrated D...
    Speaker: John Zuris, PhD
    Presented at: GenScript Gene & Cell Engineering Virtual Summit
    3
     
     
  • JUL 22, 2021 | 12:00 PM
    Building More Useful CRISPR-Cas Technologies
    Building More Useful CRISPR-Cas Technologies
    Learning Objectives: 1. Understand engineered CRISPR-Cas variants overcome natural limitations of wild-type enzymes 2. Understand the genome is now nearly completely editable 3. Understand m...
    Speaker: Ben Kleinstiver, PhD
    Presented at: GenScript Gene & Cell Engineering Virtual Summit
    2
    JUL 01, 2021 | 8:00 AM
    Quality control advancements in therapeutic genome editing and biomanufacturing
    Quality control advancements in therapeutic genome editing and...
    Date: July 1, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) What’s driving precision-medicine innovations? New advances in gene and cell therapy manufacturing, along with more...
    Speaker: Imen Mestiri, PhD
    Sponsored By: Genomic Vision
    9
    JUN 30, 2021 | 7:00 AM
    Optical Genome Mapping: Unrivaled Structural Variant Detection for Cancer Research and Precision Medicine
    Optical Genome Mapping: Unrivaled Structural Variant Detection...
    Date: June 30, 2021 Time: 7:00am (PDT), 8:00am (EDT) Join us to discover how Optical genome mapping (OGM) mapping can help you in the characterization of complex samples. OGM is a non-sequen...
    Speaker: Yannick Delpu, PhD , Darren "Ben" Finlay, PhD
    Sponsored By: Bionano
    8
    JUN 29, 2021 | 10:00 AM
    Approaches for Rapid Genomic Surveillance to Identify SARS-CoV-2 Variants
    Approaches for Rapid Genomic Surveillance to Identify SARS-CoV-2...
    Date: June 29, 2021 Time: 10:00am (PDT), 1:00pm (EDT) SARS-CoV-2 continues to evolve and surveillance of variants is a necessity. Routine analysis of genetic sequence data allows the identif...
    Speaker: Donna Wolk, MHA, PhD, D(ABMM)
    Sponsored By: Diasorin
    16
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