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    Nucleic Acid

    Nucleic acids ( i.e., DNA and RNA) are the key macromolecules of life, composed of nucleotide monomers that consist of a 5-carbon sugar ( ribose for DNA and deoxyribose for RNA), a base (adenine, guanine, thymine, cytosine, or uracil), and a phosphate group.

    Webinars

    • APR 18, 2024 | 7:00 AM
      Beyond CRISPR Basics: Molecular Biology Innovations in Cell Engineering
      Beyond CRISPR Basics: Molecular Biology Innovations in Cell Engineering
      Abstract: Beginning with a brief historical overview of CRISPR, we will trace its evolution to the current state of gene engineering, highlighting its pivotal role in reshaping the landscape...
      Speaker: Travis Hardcastle
      Sponsored By: Thermo Fisher Scientific
      23
      MAR 20, 2024 | 8:00 AM
      Introducing the first dual reporter application for Luminex xMAP INTELLIFLEX DR-SE Instruments:  measurement of phosphorylated and total proteins on the same bead
      Introducing the first dual reporter application for Luminex xMAP...
      Luminex and Thermo Fisher Scientific are excited to feature two presentations highlighting the advanced features of the Invitrogen™ Luminex® xMAP® INTELLIFLEX™ DR-SE inst...
      Speaker: Sherry Dunbar, Ph.D., MBA , Sigrun Badrnya, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      11
      DEC 12, 2023 | 10:00 AM
      Characterization of LNPs via Density Matching AUC
      Characterization of LNPs via Density Matching AUC
      Determining the purity of LNPs loaded with nucleic acids can be challenging due to their size heterogeneity. Here, we present analytical ultracentrifugation (AUC) methods that use multiwavel...
      Speaker: Amy Henrickson
      Sponsored By: Beckman Coulter Life Sciences
      11
      NOV 08, 2023 | 8:00 AM
      AUC (Analytical Ultracentrifugation) capabilities for the analysis of gene therapy products
      AUC (Analytical Ultracentrifugation) capabilities for the analysis...
      AAVs and LNPs are complementary gene transfer systems with respect to immunogenicity, tissue tropism and types of nucleic acids, which can be delivered. AUC provides a wealth of orthogonal a...
      Speaker: Alexander Bepperling, PhD
      Sponsored By: Beckman Coulter Life Sciences
      6
      NOV 08, 2023 | 8:00 AM
      Time to revisit RNA preservation - Synergy Blood Collection Tube
      Time to revisit RNA preservation - Synergy Blood Collection Tube
      SiO2 Materials Science is a company with deep roots in materials science and engineering and a track record of innovative packaging products for pharmaceutical drugs. The company also manufa...
      Speaker: Chris Weikart, PhD , Ishfaq Maksud
      Sponsored By: SIO2 Materials Science
      8
      NOVEMBER 02, 2023 2:00 PM ET
      Sample Preparation Solutions for Environmental and Agricultural Research
      Sample Preparation Solutions for Environmental and Agricultural...
      Sample preparation solutions for environmental and agricultural research using homogenizers and kits....
      Speaker: Gavin Wang , Maria Burton
      Presented at: The Thomas Experts Series & The Thomas Center Labs
      Sponsored By: MP Bio
      9
      OCT 31, 2023 | 11:00 AM
      Optimizing Experimental Design for Gene Editing Success: tips and tricks to increase efficiency and improve outcomes
      Optimizing Experimental Design for Gene Editing Success: tips...
      With the promise of targeted gene editing and CRISPR technologies comes increasing experimental complexity and design challenges. Success in gene editing experiments may depend on many varia...
      Speaker: Fuqiang Chen, Ph.D.
      Sponsored By: MilliporeSigma
      8
      SEP 14, 2023 | 9:00 AM
      Demystifying Exosome Isolation: Best Practices and Centrifuge Selection Strategies
      Demystifying Exosome Isolation: Best Practices and Centrifuge...
      Small extracellular vesicles (EVs) and Exosomes released by cells, have gained significant interest in biomedical research due to their potential applications in diagnostics and therapeutics...
      Speaker: Marzieh Mousavi
      Sponsored By: Thermo Fisher Scientific
      19
      SEP 13, 2023 | 10:00 AM
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
      Liquid biopsy is an emerging area of clinical research, particularly in the context of cancer. As a minimally invasive complementary or alternative approach to tissue biopsies, liquid biopsi...
      Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
      Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
      Sponsored By: Thermo Fisher Scientific
      20
      September 13 at 10:00am CEST, 9:00am BST
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
      Webinar airing too early or too late for you? This webinar will also be available: September 13 at 10:00am PDT, 1:00pm EDT https://www.labroots.com/ms/webinar/dpcr-liquidbiopsy-panel-2 and S...
      Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
      Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
      Sponsored By: Thermo Fisher Scientific
      12
      September 13 at 9:30am IST, 12:00pm CST/SGT
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
      Liquid biopsy is an emerging area of clinical research, particularly in the context of cancer. As a minimally invasive complementary or alternative approach to tissue biopsies, liquid biopsi...
      Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
      Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
      Sponsored By: Thermo Fisher Scientific
      12
      SEP 12, 2023 | 9:00 AM
      Understanding immune activation and evasion in the tumor microenvironment: Why lymphocyte relationships matter
      Understanding immune activation and evasion in the tumor microenvironment:...
      In recent years, significant progress has been made in tissue-based technology, opening up unparalleled opportunities for identifying disease process biomarkers and gaining invaluable insigh...
      Speaker: Gourab Chatterjee
      Sponsored By: Ultivue
      20
      SEP 07, 2023 | 8:00 AM
      Quest Diagnostics with Covaris launches automated NGS platform, optimizing workflows to maximize lab economics
      Quest Diagnostics with Covaris launches automated NGS platform,...
      Advancements in Next Generation Sequencing (NGS) are changing the genomics industry, favoring those with scale. Quest Diagnostics' clinical expertise, combined with our infrastructure, p...
      Speaker: Ryan Serra, BSc. and Ph.D. , Matthew Gallen, MPH , Sameer Vasantgadkar
      Sponsored By: Covaris, Inc.
      12
      JUN 29, 2023 | 8:00 AM
      THE IRRESISTIBLE PULL OF PARAMAGNETIC BEADS
      THE IRRESISTIBLE PULL OF PARAMAGNETIC BEADS
      Molecular biology workflows within drug discovery and development pipelines rely on the efficient extraction and cleanup of nucleic acids. Maximizing recovery, consistency and speed is achie...
      Speaker: Lori Euler
      Sponsored By: Beckman Coulter Life Sciences
      7
      JUN 28, 2023 | 8:00 AM
      Talking diagnostics: A "culture" moving to PCR
      Talking diagnostics: A "culture" moving to PCR
      Laboratories performing diagnostic testing for infectious diseases have an obligation to meet the needs of multiple patient populations. With infectious disease, time it takes for reporting...
      Speaker: Dr. Michael Perry
      Sponsored By: Thermo Fisher Scientific
      34
      JUN 27, 2023 | 11:00 AM
      Advancing cancer research with tools for liquid biopsy
      Advancing cancer research with tools for liquid biopsy
      Cancer is a leading cause of death worldwide, accounting for nearly 10 million deaths in 2020, or nearly one in six deaths, according to the World Health Organization. Many cancers can be cu...
      Speaker: Laure Jobert, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      26
      JUN 13, 2023 | 10:00 AM
      Advanced digital PCR applications for cell and gene therapy research: Viral vector quantification and CAR-T cell monitoring
      Advanced digital PCR applications for cell and gene therapy research:...
      Achieving consistent AAV quantification with the QuantStudio Absolute Q AutoRun dPCR Suite Accurate quantification of AAV vectors is crucial for quality control and timely decision-making du...
      Speaker: Kimberly Gomez , Himani Patel , Raquel Muñoz-Garcia, PhD
      Sponsored By: Thermo Fisher Scientific
      14
      JUN 08, 2023 | 10:40 AM
      Analytical Validation for qPCR with Live Q&A
      Analytical Validation for qPCR with Live Q&A
      Real-time PCR, also known as quantitative PCR (qPCR), is the gold standard for sensitive, specific detection and quantification of nucleic acid targets. This qPCR technology as offered by Th...
      Speaker: Victoria Quiett
      Presented at: 2023 Lab Compliance Connect
      13
      MAY 11, 2023 | 8:00 AM
      Benefits of Real-Time PCR Multiplexing
      Benefits of Real-Time PCR Multiplexing
      Date: May 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics and vaccines....
      Speaker: John Pfeifer, PhD
      Sponsored By: Thermo Fisher Scientific
      22
      APR 11, 2023 | 8:00 AM
      Webinar: Dystrophin Quantification in Preclinical and Clinical Settings
      Webinar: Dystrophin Quantification in Preclinical and Clinical...
      Date: April 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Duchenne muscular dystrophy is caused by mutations in the dystrophin encoding DMD gene that disrupt the reading frame. M...
      Speaker: Annemieke Aartsma-Rus, PhD
      Sponsored By: Bio-Techne
      18
      APR 06, 2023 | 10:00 AM
      Don't fear the robot: Lab automation for high-throughput discovery science
      Don't fear the robot: Lab automation for high-throughput discovery...
      Date: April 06, 2023 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) Join this webinar to learn how Carlos Goller has designed undergraduate and graduate courses to teach high-throughput di...
      Speaker: Carlos Goller
      Sponsored By: Opentrons
      17
      MAR 08, 2023 | 9:00 AM
      Boost microbial detection sensitivity with unbiased human host DNA and RNA depletion
      Boost microbial detection sensitivity with unbiased human host...
      Date: March 08, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) An unbiased characterization of the human microbiome is crucial to a more complete understanding of microbial effect on h...
      Speaker: Zenas George, Ph.D.
      Sponsored By: Jumpcode Genomics
      8
      FEB 15, 2023 | 7:00 AM
      Scattering Microscopy: Label-Free Imaging Approaches
      Scattering Microscopy: Label-Free Imaging Approaches
      Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
      Speaker: Philipp Kukura , Francesco Reina , Matthew Kose-Dunn , Phil Allen
      Sponsored By: Teledyne Photometrics
      28
      JAN 18, 2023 | 9:00 AM
      Elucidating the composition of CRISPR-Cas12f1 complexes using mass photometry
      Elucidating the composition of CRISPR-Cas12f1 complexes using...
      Date: January 18, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) CRISPR-Cas systems are widely used in genome engineering applications. They contain RNA-guided ribonucleoprotein (RNP)...
      Speaker: Selgar Henkel-Heinecke
      Sponsored By: Refeyn
      13
    APR 18, 2024 | 7:00 AM
    Beyond CRISPR Basics: Molecular Biology Innovations in Cell Engineering
    Beyond CRISPR Basics: Molecular Biology Innovations in Cell Engineering
    Abstract: Beginning with a brief historical overview of CRISPR, we will trace its evolution to the current state of gene engineering, highlighting its pivotal role in reshaping the landscape...
    Speaker: Travis Hardcastle
    Sponsored By: Thermo Fisher Scientific
    23
    MAR 20, 2024 | 8:00 AM
    Introducing the first dual reporter application for Luminex xMAP INTELLIFLEX DR-SE Instruments:  measurement of phosphorylated and total proteins on the same bead
    Introducing the first dual reporter application for Luminex xMAP...
    Luminex and Thermo Fisher Scientific are excited to feature two presentations highlighting the advanced features of the Invitrogen™ Luminex® xMAP® INTELLIFLEX™ DR-SE inst...
    Speaker: Sherry Dunbar, Ph.D., MBA , Sigrun Badrnya, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    11
    DEC 12, 2023 | 10:00 AM
    Characterization of LNPs via Density Matching AUC
    Characterization of LNPs via Density Matching AUC
    Determining the purity of LNPs loaded with nucleic acids can be challenging due to their size heterogeneity. Here, we present analytical ultracentrifugation (AUC) methods that use multiwavel...
    Speaker: Amy Henrickson
    Sponsored By: Beckman Coulter Life Sciences
    11
    NOV 08, 2023 | 8:00 AM
    AUC (Analytical Ultracentrifugation) capabilities for the analysis of gene therapy products
    AUC (Analytical Ultracentrifugation) capabilities for the analysis...
    AAVs and LNPs are complementary gene transfer systems with respect to immunogenicity, tissue tropism and types of nucleic acids, which can be delivered. AUC provides a wealth of orthogonal a...
    Speaker: Alexander Bepperling, PhD
    Sponsored By: Beckman Coulter Life Sciences
    6
    NOV 08, 2023 | 8:00 AM
    Time to revisit RNA preservation - Synergy Blood Collection Tube
    Time to revisit RNA preservation - Synergy Blood Collection Tube
    SiO2 Materials Science is a company with deep roots in materials science and engineering and a track record of innovative packaging products for pharmaceutical drugs. The company also manufa...
    Speaker: Chris Weikart, PhD , Ishfaq Maksud
    Sponsored By: SIO2 Materials Science
    8
     
     
  • NOVEMBER 02, 2023 2:00 PM ET
    Sample Preparation Solutions for Environmental and Agricultural Research
    Sample Preparation Solutions for Environmental and Agricultural...
    Sample preparation solutions for environmental and agricultural research using homogenizers and kits....
    Speaker: Gavin Wang , Maria Burton
    Presented at: The Thomas Experts Series & The Thomas Center Labs
    Sponsored By: MP Bio
    9
    OCT 31, 2023 | 11:00 AM
    Optimizing Experimental Design for Gene Editing Success: tips and tricks to increase efficiency and improve outcomes
    Optimizing Experimental Design for Gene Editing Success: tips...
    With the promise of targeted gene editing and CRISPR technologies comes increasing experimental complexity and design challenges. Success in gene editing experiments may depend on many varia...
    Speaker: Fuqiang Chen, Ph.D.
    Sponsored By: MilliporeSigma
    8
    SEP 14, 2023 | 9:00 AM
    Demystifying Exosome Isolation: Best Practices and Centrifuge Selection Strategies
    Demystifying Exosome Isolation: Best Practices and Centrifuge...
    Small extracellular vesicles (EVs) and Exosomes released by cells, have gained significant interest in biomedical research due to their potential applications in diagnostics and therapeutics...
    Speaker: Marzieh Mousavi
    Sponsored By: Thermo Fisher Scientific
    19
    SEP 13, 2023 | 10:00 AM
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
    Liquid biopsy is an emerging area of clinical research, particularly in the context of cancer. As a minimally invasive complementary or alternative approach to tissue biopsies, liquid biopsi...
    Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
    Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
    Sponsored By: Thermo Fisher Scientific
    20
    September 13 at 10:00am CEST, 9:00am BST
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
    Webinar airing too early or too late for you? This webinar will also be available: September 13 at 10:00am PDT, 1:00pm EDT https://www.labroots.com/ms/webinar/dpcr-liquidbiopsy-panel-2 and S...
    Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
    Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
    Sponsored By: Thermo Fisher Scientific
    12
     
     
  • September 13 at 9:30am IST, 12:00pm CST/SGT
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
    Liquid biopsy is an emerging area of clinical research, particularly in the context of cancer. As a minimally invasive complementary or alternative approach to tissue biopsies, liquid biopsi...
    Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
    Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
    Sponsored By: Thermo Fisher Scientific
    12
    SEP 12, 2023 | 9:00 AM
    Understanding immune activation and evasion in the tumor microenvironment: Why lymphocyte relationships matter
    Understanding immune activation and evasion in the tumor microenvironment:...
    In recent years, significant progress has been made in tissue-based technology, opening up unparalleled opportunities for identifying disease process biomarkers and gaining invaluable insigh...
    Speaker: Gourab Chatterjee
    Sponsored By: Ultivue
    20
    SEP 07, 2023 | 8:00 AM
    Quest Diagnostics with Covaris launches automated NGS platform, optimizing workflows to maximize lab economics
    Quest Diagnostics with Covaris launches automated NGS platform,...
    Advancements in Next Generation Sequencing (NGS) are changing the genomics industry, favoring those with scale. Quest Diagnostics' clinical expertise, combined with our infrastructure, p...
    Speaker: Ryan Serra, BSc. and Ph.D. , Matthew Gallen, MPH , Sameer Vasantgadkar
    Sponsored By: Covaris, Inc.
    12
    JUN 29, 2023 | 8:00 AM
    THE IRRESISTIBLE PULL OF PARAMAGNETIC BEADS
    THE IRRESISTIBLE PULL OF PARAMAGNETIC BEADS
    Molecular biology workflows within drug discovery and development pipelines rely on the efficient extraction and cleanup of nucleic acids. Maximizing recovery, consistency and speed is achie...
    Speaker: Lori Euler
    Sponsored By: Beckman Coulter Life Sciences
    7
    JUN 28, 2023 | 8:00 AM
    Talking diagnostics: A "culture" moving to PCR
    Talking diagnostics: A "culture" moving to PCR
    Laboratories performing diagnostic testing for infectious diseases have an obligation to meet the needs of multiple patient populations. With infectious disease, time it takes for reporting...
    Speaker: Dr. Michael Perry
    Sponsored By: Thermo Fisher Scientific
    34
     
     
  • JUN 27, 2023 | 11:00 AM
    Advancing cancer research with tools for liquid biopsy
    Advancing cancer research with tools for liquid biopsy
    Cancer is a leading cause of death worldwide, accounting for nearly 10 million deaths in 2020, or nearly one in six deaths, according to the World Health Organization. Many cancers can be cu...
    Speaker: Laure Jobert, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    26
    JUN 13, 2023 | 10:00 AM
    Advanced digital PCR applications for cell and gene therapy research: Viral vector quantification and CAR-T cell monitoring
    Advanced digital PCR applications for cell and gene therapy research:...
    Achieving consistent AAV quantification with the QuantStudio Absolute Q AutoRun dPCR Suite Accurate quantification of AAV vectors is crucial for quality control and timely decision-making du...
    Speaker: Kimberly Gomez , Himani Patel , Raquel Muñoz-Garcia, PhD
    Sponsored By: Thermo Fisher Scientific
    14
    JUN 08, 2023 | 10:40 AM
    Analytical Validation for qPCR with Live Q&A
    Analytical Validation for qPCR with Live Q&A
    Real-time PCR, also known as quantitative PCR (qPCR), is the gold standard for sensitive, specific detection and quantification of nucleic acid targets. This qPCR technology as offered by Th...
    Speaker: Victoria Quiett
    Presented at: 2023 Lab Compliance Connect
    13
    MAY 11, 2023 | 8:00 AM
    Benefits of Real-Time PCR Multiplexing
    Benefits of Real-Time PCR Multiplexing
    Date: May 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics and vaccines....
    Speaker: John Pfeifer, PhD
    Sponsored By: Thermo Fisher Scientific
    22
    APR 11, 2023 | 8:00 AM
    Webinar: Dystrophin Quantification in Preclinical and Clinical Settings
    Webinar: Dystrophin Quantification in Preclinical and Clinical...
    Date: April 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Duchenne muscular dystrophy is caused by mutations in the dystrophin encoding DMD gene that disrupt the reading frame. M...
    Speaker: Annemieke Aartsma-Rus, PhD
    Sponsored By: Bio-Techne
    18
     
     
  • APR 06, 2023 | 10:00 AM
    Don't fear the robot: Lab automation for high-throughput discovery science
    Don't fear the robot: Lab automation for high-throughput discovery...
    Date: April 06, 2023 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) Join this webinar to learn how Carlos Goller has designed undergraduate and graduate courses to teach high-throughput di...
    Speaker: Carlos Goller
    Sponsored By: Opentrons
    17
    MAR 08, 2023 | 9:00 AM
    Boost microbial detection sensitivity with unbiased human host DNA and RNA depletion
    Boost microbial detection sensitivity with unbiased human host...
    Date: March 08, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) An unbiased characterization of the human microbiome is crucial to a more complete understanding of microbial effect on h...
    Speaker: Zenas George, Ph.D.
    Sponsored By: Jumpcode Genomics
    8
    FEB 15, 2023 | 7:00 AM
    Scattering Microscopy: Label-Free Imaging Approaches
    Scattering Microscopy: Label-Free Imaging Approaches
    Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
    Speaker: Philipp Kukura , Francesco Reina , Matthew Kose-Dunn , Phil Allen
    Sponsored By: Teledyne Photometrics
    28
    JAN 18, 2023 | 9:00 AM
    Elucidating the composition of CRISPR-Cas12f1 complexes using mass photometry
    Elucidating the composition of CRISPR-Cas12f1 complexes using...
    Date: January 18, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) CRISPR-Cas systems are widely used in genome engineering applications. They contain RNA-guided ribonucleoprotein (RNP)...
    Speaker: Selgar Henkel-Heinecke
    Sponsored By: Refeyn
    13
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