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    Nucleic Acid

    Nucleic acids ( i.e., DNA and RNA) are the key macromolecules of life, composed of nucleotide monomers that consist of a 5-carbon sugar ( ribose for DNA and deoxyribose for RNA), a base (adenine, guanine, thymine, cytosine, or uracil), and a phosphate group.

    Webinars

    • On Demand
      CRISPR/Cas Biosensing System: Opportunities and Challenges
      CRISPR/Cas Biosensing System: Opportunities and Challenges
      Rather than being famous in gene editing field only, by revealing the collateral cleavage activity of Cas12a, Cas13a, Cas14 effectors, and more, Clustered Regularly Interspaced Short Palindr...
      Speaker: Guozhen Liu, PhD
      Presented at: CRISPR Virtual Event Series 2023
      1
      OCT 31, 2023 | 11:00 AM
      Optimizing Experimental Design for Gene Editing Success: tips and tricks to increase efficiency and improve outcomes
      Optimizing Experimental Design for Gene Editing Success: tips...
      With the promise of targeted gene editing and CRISPR technologies comes increasing experimental complexity and design challenges. Success in gene editing experiments may depend on many varia...
      Speaker: Fuqiang Chen, Ph.D.
      Sponsored By: MilliporeSigma
      8
      SEP 14, 2023 | 9:00 AM
      Demystifying Exosome Isolation: Best Practices and Centrifuge Selection Strategies
      Demystifying Exosome Isolation: Best Practices and Centrifuge...
      Small extracellular vesicles (EVs) and Exosomes released by cells, have gained significant interest in biomedical research due to their potential applications in diagnostics and therapeutics...
      Speaker: Marzieh Mousavi
      Sponsored By: Thermo Fisher Scientific
      19
      SEP 13, 2023 | 10:00 AM
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
      Liquid biopsy is an emerging area of clinical research, particularly in the context of cancer. As a minimally invasive complementary or alternative approach to tissue biopsies, liquid biopsi...
      Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
      Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
      Sponsored By: Thermo Fisher Scientific
      20
      September 13 at 10:00am CEST, 9:00am BST
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
      Webinar airing too early or too late for you? This webinar will also be available: September 13 at 10:00am PDT, 1:00pm EDT https://www.labroots.com/ms/webinar/dpcr-liquidbiopsy-panel-2 and S...
      Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
      Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
      Sponsored By: Thermo Fisher Scientific
      12
      September 13 at 9:30am IST, 12:00pm CST/SGT
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
      Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
      Liquid biopsy is an emerging area of clinical research, particularly in the context of cancer. As a minimally invasive complementary or alternative approach to tissue biopsies, liquid biopsi...
      Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
      Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
      Sponsored By: Thermo Fisher Scientific
      12
      SEP 12, 2023 | 9:00 AM
      Understanding immune activation and evasion in the tumor microenvironment: Why lymphocyte relationships matter
      Understanding immune activation and evasion in the tumor microenvironment:...
      In recent years, significant progress has been made in tissue-based technology, opening up unparalleled opportunities for identifying disease process biomarkers and gaining invaluable insigh...
      Speaker: Gourab Chatterjee
      Sponsored By: Ultivue
      20
      SEP 07, 2023 | 8:00 AM
      Quest Diagnostics with Covaris launches automated NGS platform, optimizing workflows to maximize lab economics
      Quest Diagnostics with Covaris launches automated NGS platform,...
      Advancements in Next Generation Sequencing (NGS) are changing the genomics industry, favoring those with scale. Quest Diagnostics' clinical expertise, combined with our infrastructure, p...
      Speaker: Ryan Serra, BSc. and Ph.D. , Matthew Gallen, MPH , Sameer Vasantgadkar
      Sponsored By: Covaris, Inc.
      12
      JUN 29, 2023 | 8:00 AM
      THE IRRESISTIBLE PULL OF PARAMAGNETIC BEADS
      THE IRRESISTIBLE PULL OF PARAMAGNETIC BEADS
      Molecular biology workflows within drug discovery and development pipelines rely on the efficient extraction and cleanup of nucleic acids. Maximizing recovery, consistency and speed is achie...
      Speaker: Lori Euler
      Sponsored By: Beckman Coulter Life Sciences
      7
      JUN 28, 2023 | 8:00 AM
      Talking diagnostics: A "culture" moving to PCR
      Talking diagnostics: A "culture" moving to PCR
      Laboratories performing diagnostic testing for infectious diseases have an obligation to meet the needs of multiple patient populations. With infectious disease, time it takes for reporting...
      Speaker: Dr. Michael Perry
      Sponsored By: Thermo Fisher Scientific
      34
      JUN 27, 2023 | 11:00 AM
      Advancing cancer research with tools for liquid biopsy
      Advancing cancer research with tools for liquid biopsy
      Cancer is a leading cause of death worldwide, accounting for nearly 10 million deaths in 2020, or nearly one in six deaths, according to the World Health Organization. Many cancers can be cu...
      Speaker: Laure Jobert, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      26
      JUN 13, 2023 | 10:00 AM
      Advanced digital PCR applications for cell and gene therapy research: Viral vector quantification and CAR-T cell monitoring
      Advanced digital PCR applications for cell and gene therapy research:...
      Achieving consistent AAV quantification with the QuantStudio Absolute Q AutoRun dPCR Suite Accurate quantification of AAV vectors is crucial for quality control and timely decision-making du...
      Speaker: Kimberly Gomez , Himani Patel , Raquel Muñoz-Garcia, PhD
      Sponsored By: Thermo Fisher Scientific
      14
      JUN 08, 2023 | 10:40 AM
      Analytical Validation for qPCR with Live Q&A
      Analytical Validation for qPCR with Live Q&A
      Real-time PCR, also known as quantitative PCR (qPCR), is the gold standard for sensitive, specific detection and quantification of nucleic acid targets. This qPCR technology as offered by Th...
      Speaker: Victoria Quiett
      Presented at: 2023 Lab Compliance Connect
      13
      MAY 11, 2023 | 8:00 AM
      Benefits of Real-Time PCR Multiplexing
      Benefits of Real-Time PCR Multiplexing
      Date: May 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics and vaccines....
      Speaker: John Pfeifer, PhD
      Sponsored By: Thermo Fisher Scientific
      22
      APR 11, 2023 | 8:00 AM
      Webinar: Dystrophin Quantification in Preclinical and Clinical Settings
      Webinar: Dystrophin Quantification in Preclinical and Clinical...
      Date: April 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Duchenne muscular dystrophy is caused by mutations in the dystrophin encoding DMD gene that disrupt the reading frame. M...
      Speaker: Annemieke Aartsma-Rus, PhD
      Sponsored By: Bio-Techne
      18
      APR 06, 2023 | 10:00 AM
      Don't fear the robot: Lab automation for high-throughput discovery science
      Don't fear the robot: Lab automation for high-throughput discovery...
      Date: April 06, 2023 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) Join this webinar to learn how Carlos Goller has designed undergraduate and graduate courses to teach high-throughput di...
      Speaker: Carlos Goller
      Sponsored By: Opentrons
      17
      MAR 08, 2023 | 9:00 AM
      Boost microbial detection sensitivity with unbiased human host DNA and RNA depletion
      Boost microbial detection sensitivity with unbiased human host...
      Date: March 08, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) An unbiased characterization of the human microbiome is crucial to a more complete understanding of microbial effect on h...
      Speaker: Zenas George, Ph.D.
      Sponsored By: Jumpcode Genomics
      8
      FEB 15, 2023 | 7:00 AM
      Scattering Microscopy: Label-Free Imaging Approaches
      Scattering Microscopy: Label-Free Imaging Approaches
      Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
      Speaker: Philipp Kukura , Francesco Reina , Matthew Kose-Dunn , Phil Allen
      Sponsored By: Teledyne Photometrics
      28
      JAN 18, 2023 | 9:00 AM
      Elucidating the composition of CRISPR-Cas12f1 complexes using mass photometry
      Elucidating the composition of CRISPR-Cas12f1 complexes using...
      Date: January 18, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) CRISPR-Cas systems are widely used in genome engineering applications. They contain RNA-guided ribonucleoprotein (RNP)...
      Speaker: Selgar Henkel-Heinecke
      Sponsored By: Refeyn
      13
      DEC 08, 2022 | 1:00 PM
      Accelerate your IVD assay development with the unique support of the Dynabeads team
      Accelerate your IVD assay development with the unique support...
      Date: December 08, 2022 Time: 12:00pm (PST), 3:00pm (EST), 9:00pm (CET) Register for our upcoming webinar “Accelerate your IVD assay development with the unique support of the Dynabead...
      Speaker: Dr. Hui Zhang , Erling Sigurd Finne
      Sponsored By: Thermo Fisher Scientific
      10
      NOV 15, 2022 | 9:00 AM
      Go with the flow for rapid viscosity results with Honeybun
      Go with the flow for rapid viscosity results with Honeybun
      Problem Measuring viscosity is critical to knowing if your protein and formulation will be well-behaved, or if they will really disrupt the flow of things during administration. Too bad most...
      Speaker: Kevin Lance, Ph.D.
      Sponsored By: Unchained Labs
      6
      NOV 15, 2022 | 8:00 AM
      Beyond SARS-CoV-2, application of Absolute Q digital PCR system for waterborne pathogens
      Beyond SARS-CoV-2, application of Absolute Q digital PCR system...
      Date: November 15, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Waterborne pathogens lead to deteriorating water quality and challenge global health and economy. The latest U.S. Envi...
      Speaker: Huiyun Wu
      Sponsored By: Thermo Fisher Scientific
      16
      NOV 08, 2022 | 9:00 AM
      Power up your LNP & AAV sample prep with Unagi
      Power up your LNP & AAV sample prep with Unagi
      Date: October 27, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) The Problem Buffer exchange is a critical step to developing optimal vectors and payloads. Compared to biologics, deve...
      Speaker: Del Ray Jackson
      Sponsored By: Unchained Labs
      9
      NOV 02, 2022 | 7:30 AM
      Keynote Presentation: Engineered LwaCas13a with Enhanced Collateral Activity for Nucleic Acid Detection
      Keynote Presentation: Engineered LwaCas13a with Enhanced Collateral...
      Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 13 (Cas13) has been rapidly developed for nucleic-acid-based diagnostics by using its chara...
      Speaker: Xue Sherry Gao, PhD
      Presented at: CRISPR Virtual Event Series 2022
      5
    On Demand
    CRISPR/Cas Biosensing System: Opportunities and Challenges
    CRISPR/Cas Biosensing System: Opportunities and Challenges
    Rather than being famous in gene editing field only, by revealing the collateral cleavage activity of Cas12a, Cas13a, Cas14 effectors, and more, Clustered Regularly Interspaced Short Palindr...
    Speaker: Guozhen Liu, PhD
    Presented at: CRISPR Virtual Event Series 2023
    1
    OCT 31, 2023 | 11:00 AM
    Optimizing Experimental Design for Gene Editing Success: tips and tricks to increase efficiency and improve outcomes
    Optimizing Experimental Design for Gene Editing Success: tips...
    With the promise of targeted gene editing and CRISPR technologies comes increasing experimental complexity and design challenges. Success in gene editing experiments may depend on many varia...
    Speaker: Fuqiang Chen, Ph.D.
    Sponsored By: MilliporeSigma
    8
    SEP 14, 2023 | 9:00 AM
    Demystifying Exosome Isolation: Best Practices and Centrifuge Selection Strategies
    Demystifying Exosome Isolation: Best Practices and Centrifuge...
    Small extracellular vesicles (EVs) and Exosomes released by cells, have gained significant interest in biomedical research due to their potential applications in diagnostics and therapeutics...
    Speaker: Marzieh Mousavi
    Sponsored By: Thermo Fisher Scientific
    19
    SEP 13, 2023 | 10:00 AM
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
    Liquid biopsy is an emerging area of clinical research, particularly in the context of cancer. As a minimally invasive complementary or alternative approach to tissue biopsies, liquid biopsi...
    Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
    Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
    Sponsored By: Thermo Fisher Scientific
    20
    September 13 at 10:00am CEST, 9:00am BST
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
    Webinar airing too early or too late for you? This webinar will also be available: September 13 at 10:00am PDT, 1:00pm EDT https://www.labroots.com/ms/webinar/dpcr-liquidbiopsy-panel-2 and S...
    Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
    Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
    Sponsored By: Thermo Fisher Scientific
    12
     
     
  • September 13 at 9:30am IST, 12:00pm CST/SGT
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research
    Webinar Series Part 2: The Future of Cutting-Edge Genomic Technologies...
    Liquid biopsy is an emerging area of clinical research, particularly in the context of cancer. As a minimally invasive complementary or alternative approach to tissue biopsies, liquid biopsi...
    Speaker: Dr. Eloisa Jantus Lewintre, PhD , Dr. Gorka Alkorta-Aranburu , Matteo Allegretti, PhD
    Presented at: The Future of Cutting-Edge Genomic Technologies for Liquid Biopsy Cancer Research​
    Sponsored By: Thermo Fisher Scientific
    12
    SEP 12, 2023 | 9:00 AM
    Understanding immune activation and evasion in the tumor microenvironment: Why lymphocyte relationships matter
    Understanding immune activation and evasion in the tumor microenvironment:...
    In recent years, significant progress has been made in tissue-based technology, opening up unparalleled opportunities for identifying disease process biomarkers and gaining invaluable insigh...
    Speaker: Gourab Chatterjee
    Sponsored By: Ultivue
    20
    SEP 07, 2023 | 8:00 AM
    Quest Diagnostics with Covaris launches automated NGS platform, optimizing workflows to maximize lab economics
    Quest Diagnostics with Covaris launches automated NGS platform,...
    Advancements in Next Generation Sequencing (NGS) are changing the genomics industry, favoring those with scale. Quest Diagnostics' clinical expertise, combined with our infrastructure, p...
    Speaker: Ryan Serra, BSc. and Ph.D. , Matthew Gallen, MPH , Sameer Vasantgadkar
    Sponsored By: Covaris, Inc.
    12
    JUN 29, 2023 | 8:00 AM
    THE IRRESISTIBLE PULL OF PARAMAGNETIC BEADS
    THE IRRESISTIBLE PULL OF PARAMAGNETIC BEADS
    Molecular biology workflows within drug discovery and development pipelines rely on the efficient extraction and cleanup of nucleic acids. Maximizing recovery, consistency and speed is achie...
    Speaker: Lori Euler
    Sponsored By: Beckman Coulter Life Sciences
    7
    JUN 28, 2023 | 8:00 AM
    Talking diagnostics: A "culture" moving to PCR
    Talking diagnostics: A "culture" moving to PCR
    Laboratories performing diagnostic testing for infectious diseases have an obligation to meet the needs of multiple patient populations. With infectious disease, time it takes for reporting...
    Speaker: Dr. Michael Perry
    Sponsored By: Thermo Fisher Scientific
    34
     
     
  • JUN 27, 2023 | 11:00 AM
    Advancing cancer research with tools for liquid biopsy
    Advancing cancer research with tools for liquid biopsy
    Cancer is a leading cause of death worldwide, accounting for nearly 10 million deaths in 2020, or nearly one in six deaths, according to the World Health Organization. Many cancers can be cu...
    Speaker: Laure Jobert, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    26
    JUN 13, 2023 | 10:00 AM
    Advanced digital PCR applications for cell and gene therapy research: Viral vector quantification and CAR-T cell monitoring
    Advanced digital PCR applications for cell and gene therapy research:...
    Achieving consistent AAV quantification with the QuantStudio Absolute Q AutoRun dPCR Suite Accurate quantification of AAV vectors is crucial for quality control and timely decision-making du...
    Speaker: Kimberly Gomez , Himani Patel , Raquel Muñoz-Garcia, PhD
    Sponsored By: Thermo Fisher Scientific
    14
    JUN 08, 2023 | 10:40 AM
    Analytical Validation for qPCR with Live Q&A
    Analytical Validation for qPCR with Live Q&A
    Real-time PCR, also known as quantitative PCR (qPCR), is the gold standard for sensitive, specific detection and quantification of nucleic acid targets. This qPCR technology as offered by Th...
    Speaker: Victoria Quiett
    Presented at: 2023 Lab Compliance Connect
    13
    MAY 11, 2023 | 8:00 AM
    Benefits of Real-Time PCR Multiplexing
    Benefits of Real-Time PCR Multiplexing
    Date: May 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics and vaccines....
    Speaker: John Pfeifer, PhD
    Sponsored By: Thermo Fisher Scientific
    22
    APR 11, 2023 | 8:00 AM
    Webinar: Dystrophin Quantification in Preclinical and Clinical Settings
    Webinar: Dystrophin Quantification in Preclinical and Clinical...
    Date: April 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Duchenne muscular dystrophy is caused by mutations in the dystrophin encoding DMD gene that disrupt the reading frame. M...
    Speaker: Annemieke Aartsma-Rus, PhD
    Sponsored By: Bio-Techne
    18
     
     
  • APR 06, 2023 | 10:00 AM
    Don't fear the robot: Lab automation for high-throughput discovery science
    Don't fear the robot: Lab automation for high-throughput discovery...
    Date: April 06, 2023 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) Join this webinar to learn how Carlos Goller has designed undergraduate and graduate courses to teach high-throughput di...
    Speaker: Carlos Goller
    Sponsored By: Opentrons
    17
    MAR 08, 2023 | 9:00 AM
    Boost microbial detection sensitivity with unbiased human host DNA and RNA depletion
    Boost microbial detection sensitivity with unbiased human host...
    Date: March 08, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) An unbiased characterization of the human microbiome is crucial to a more complete understanding of microbial effect on h...
    Speaker: Zenas George, Ph.D.
    Sponsored By: Jumpcode Genomics
    8
    FEB 15, 2023 | 7:00 AM
    Scattering Microscopy: Label-Free Imaging Approaches
    Scattering Microscopy: Label-Free Imaging Approaches
    Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
    Speaker: Philipp Kukura , Francesco Reina , Matthew Kose-Dunn , Phil Allen
    Sponsored By: Teledyne Photometrics
    28
    JAN 18, 2023 | 9:00 AM
    Elucidating the composition of CRISPR-Cas12f1 complexes using mass photometry
    Elucidating the composition of CRISPR-Cas12f1 complexes using...
    Date: January 18, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) CRISPR-Cas systems are widely used in genome engineering applications. They contain RNA-guided ribonucleoprotein (RNP)...
    Speaker: Selgar Henkel-Heinecke
    Sponsored By: Refeyn
    13
    DEC 08, 2022 | 1:00 PM
    Accelerate your IVD assay development with the unique support of the Dynabeads team
    Accelerate your IVD assay development with the unique support...
    Date: December 08, 2022 Time: 12:00pm (PST), 3:00pm (EST), 9:00pm (CET) Register for our upcoming webinar “Accelerate your IVD assay development with the unique support of the Dynabead...
    Speaker: Dr. Hui Zhang , Erling Sigurd Finne
    Sponsored By: Thermo Fisher Scientific
    10
     
     
  • NOV 15, 2022 | 9:00 AM
    Go with the flow for rapid viscosity results with Honeybun
    Go with the flow for rapid viscosity results with Honeybun
    Problem Measuring viscosity is critical to knowing if your protein and formulation will be well-behaved, or if they will really disrupt the flow of things during administration. Too bad most...
    Speaker: Kevin Lance, Ph.D.
    Sponsored By: Unchained Labs
    6
    NOV 15, 2022 | 8:00 AM
    Beyond SARS-CoV-2, application of Absolute Q digital PCR system for waterborne pathogens
    Beyond SARS-CoV-2, application of Absolute Q digital PCR system...
    Date: November 15, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Waterborne pathogens lead to deteriorating water quality and challenge global health and economy. The latest U.S. Envi...
    Speaker: Huiyun Wu
    Sponsored By: Thermo Fisher Scientific
    16
    NOV 08, 2022 | 9:00 AM
    Power up your LNP & AAV sample prep with Unagi
    Power up your LNP & AAV sample prep with Unagi
    Date: October 27, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) The Problem Buffer exchange is a critical step to developing optimal vectors and payloads. Compared to biologics, deve...
    Speaker: Del Ray Jackson
    Sponsored By: Unchained Labs
    9
    NOV 02, 2022 | 7:30 AM
    Keynote Presentation: Engineered LwaCas13a with Enhanced Collateral Activity for Nucleic Acid Detection
    Keynote Presentation: Engineered LwaCas13a with Enhanced Collateral...
    Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 13 (Cas13) has been rapidly developed for nucleic-acid-based diagnostics by using its chara...
    Speaker: Xue Sherry Gao, PhD
    Presented at: CRISPR Virtual Event Series 2022
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