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    Reporter genes

    Reporter genes are an integral component of molecular biology used to study biological pathways and processes. Reporter genes are attached to a known sequence of another gene of interest in an organism, such as a bacteria, cell, or plant. Attachment of a reporter gene allows visualization or quantification of a gene of interest. Common reporter systems used on molecular biology include luciferase and green fluorescent protein (GFP).

    Webinars

    • JUN 21, 2022 | 6:00 AM
      The future of predictive genomics in Israel
      The future of predictive genomics in Israel
      Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
      Speaker: Zisis Kozlakidis , David Hanna , Dr. Yehudit Cohen , Sowmiya Moorthie , Shai Carmi
      Sponsored By: Thermo Fisher Scientific
      27
      MAY 17, 2022 | 8:00 AM
      Novel tools for cell line engineering with CRISPR-Cas9
      Novel tools for cell line engineering with CRISPR-Cas9
      Date: May 17, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) The CRISPR-Cas9 system provides researchers a powerful tool for manipulation of cells to create disease models, reporter c...
      Speaker: Louise Baskin, M.S. Molecular Biology and Genetics
      Sponsored By: Thermo Fisher Scientific
      13
      MAY 05, 2022 | 8:00 AM
      Solve Every Hereditary Disorder Case for All Types of Genetic Variation
      Solve Every Hereditary Disorder Case for All Types of Genetic...
      Date: May 5, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CET) Despite incredible advances in genomics, the NGS data interpretation workflow for hereditary diseases remains challenging...
      Speaker: Georgios Stamoulis, Ph.D.
      Sponsored By: QIAGEN
      7
      MAR 09, 2022 | 8:00 AM
      Biosecurity for Genome Engineering: Safety First and the SeqScreen Solution for Functional Predictions
      Biosecurity for Genome Engineering: Safety First and the SeqScreen...
      Date: March 09, 2021 Time: 08:00am (PST), 11:00am (EST), 5:00pm (CEST) Bioengineering technologies hold enormous potential to solve many of our world’s pressing problems. However, they...
      Speaker: Todd Treangen, PhD , Krista Ternus, PhD, PMP , Beth Vitalis, PhD
      Sponsored By: Inscripta
      5
      DEC 07, 2021 | 8:00 AM
      T Cells in COVID-19 - Translating research findings into clinical options
      T Cells in COVID-19 - Translating research findings into clinical...
      Date: November 7, 2021 Time: 8:00am (PDT), 11:00am (EDT) Analysis of SARS-CoV-2–specific T cells is important to understand their role during acute disease and in long-term protection...
      Speaker: Lorenz Fülle, PhD , Kirsten Langeveld
      Sponsored By: Miltenyi Biotec
      10 1
      NOV 17, 2021 | 7:00 AM
      CRISPR-based transcriptional activation tools for silent genes in filamentous fungi
      CRISPR-based transcriptional activation tools for silent genes...
      DATE: November 17, 2021 TIME: 7:00am PST, 10:00am EST, 4:00pm CET Filamentous fungi are historically known to be a rich reservoir of bioactive compounds that are applied in a myriad of field...
      Speaker: Laszlo Mozsik
      Sponsored By: GenScript
      13 2
      NOV 08, 2021 | 9:00 AM
      Discovering Unforeseen Protein Assemblies with Mass Photometry
      Discovering Unforeseen Protein Assemblies with Mass Photometry
      Date: November 08, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CET) Many enzymes form homomeric complexes comprised of subunits encoded by the same gene. Assembly into homomeric...
      Speaker: Franziska Sendker
      Sponsored By: Refeyn
      4
      OCT 28, 2021 | 8:00 AM
      How to Advance Cell & Gene Therapies with an Innovative, Flexible Approach
      How to Advance Cell & Gene Therapies with an Innovative, Flexible...
      Date: October 28, 2021 Time: 8:00am (PDT), 11:00am (EDT) Today’s health industry is leveraging new, cutting-edge Cell & Gene Therapies (CGTs) to address a variety of medical illnes...
      Speaker: ​​​​​​​Chris ​Andrews , Domenico Palumberi
      Sponsored By: Dassault Systèmes
      6
      OCT 14, 2021 | 7:00 AM
      Heterologous Protein Engineering and characterization of native regulatory elements  in E. coli using Massively Parallel CRISPR Editing
      Heterologous Protein Engineering and characterization of native...
      Date: October 14, 2021 Time: 7:00am (PDT), 10:00am (EDT) Heterologous protein expression in model organisms has many applications, including protein engineering, production of industrial enz...
      Speaker: Tyson Shepherd, PhD
      Sponsored By: Inscripta
      22
      OCT 07, 2021 | 7:00 AM
      Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis
      Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common...
      DATE: October 7, 2021 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CET The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of...
      Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
      Sponsored By: Eppendorf, Promega
      12
      SEP 29, 2021 | 9:00 AM
      Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome Engineering
      Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome...
      RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 and novel RNA targeti...
      Speaker: Omar Abudayyeh, PhD , Jonathan Gootenberg, PhD
      Presented at: CRISPR Virtual Event Series 2021
      1
      SEP 09, 2021 | 7:00 AM
      Maximize your variant knowledge using the Human Gene Mutation Database (HGMD®) Professional
      Maximize your variant knowledge using the Human Gene Mutation...
      Date: September 9, 2021 Time: 7:00am (PDT), 10:00am (EDT) Founded and maintained by the Institute of Medical Genetics at Cardiff University in 1996, HGMD attempts to collate all known, publi...
      Speaker: Dr. Ruth Burton
      Sponsored By: QIAGEN
      3
      SEP 08, 2021 | 12:00 AM
      SeXX Matters for Respiratory Viral Pathogenesis and Responses to Vaccines
      SeXX Matters for Respiratory Viral Pathogenesis and Responses...
      The ongoing COVID-19 pandemic has increased awareness about sex-specific differences in immunity and outcomes following respiratory virus infections. Strong evidence of a male bias in COVID-...
      Speaker: Sabra L. Klein, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      0
      SEP 07, 2021 | 12:00 AM
      CMPK2 Restricts the Replication of Multiple Flaviviruses
      CMPK2 Restricts the Replication of Multiple Flaviviruses
      Flaviviruses are pathogens of global public health concern. They include dengue virus (DENV), West Nile virus (WNV), and zika virus (ZIKV). There are no approved US Food and Drug Administrat...
      Speaker: Maudry Laurent-Rolle, MD/PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      2
      SEP 07, 2021 | 12:00 AM
      Immune-Mediated Pathologies During Dengue Virus Infection
      Immune-Mediated Pathologies During Dengue Virus Infection
      Flaviviruses are pathogens of global public health concern. They include dengue virus (DENV), West Nile virus (WNV), and zika virus (ZIKV). There are no approved US Food and Drug Administrat...
      Speaker: Ashley St. John, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      1
      AUG 25, 2021 | 12:00 AM
      Alpha-synuclein Aggregation in Parkinson's Disease - Is it Relevant and How can we Model it?
      Alpha-synuclein Aggregation in Parkinson's Disease - Is it Relevant...
      Parkinson’s disease (PD) is the second most common neurodegenerative disorder with multiple motor and non-motor symptoms. PD is characterized by the presence on proteinaceous neuronal...
      Speaker: Andrii Domanskyi, PhD
      Presented at: Neuroscience Virtual Event Series 2021
      5
      JUL 22, 2021 | 2:45 PM
      Library-selected AAV variants can effectively translate to non-human primates in the spinal cord and cochlea
      Library-selected AAV variants can effectively translate to non-human...
      AAV peptide display libraries allow for the generation of novel variants capable of high-level transduction. We previously described an AAV peptide display library combined with a sensitive...
      Speaker: Killian S. Hanlon, PhD
      Presented at: GenScript Gene & Cell Engineering Virtual Summit
      1
      JUL 13, 2021 | 11:00 AM
      Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow
      Monoclonality Assurance Using CloneSelect Single-Cell Printer...
      Date: July 13, 2021 Time: 11:00am (PDT), 1:00pm (EDT) 1 R&D, Cell Culture & Fermentation Sciences, Bio Pharmaceutical Development, AstraZeneca, Gaithersburg, USA, 2 R&D, ADPE, Ce...
      Speaker: Gargi Roy
      Sponsored By: Molecular Devices
      9 10
      MAY 11, 2021 | 1:30 PM
      The Lifecycle of Communicating the Science panel
      The Lifecycle of Communicating the Science panel
      ...
      Speaker: Thomas F. Babor, PhD, MPH , May Berenbaum, PhD , Holly Falk-Krzesinski, PhD , Moira Gunn, PhD , Jessica Miles, PhD
      Presented at: 5th Annual Vivian Pinn Symposium
      2
      APR 22, 2021 | 6:00 AM
      Panel Presentation: Powering the precision in "precision medicine" - The role of curated data, databases and knowledge: Does a gold standard exist?
      Panel Presentation: Powering the precision in "precision medicine"...
      As NGS panels grow, test interpretation becomes more complex and time-consuming / With the explosion of NGS data, the need for consolidated databases with reliable and transparent curation a...
      Speaker: Dominic John , Simon Forbes, PhD , Shawn Bauer , Lisa Barrow-Laing
      Presented at: Genetics Week Virtual Event Series 2021
      Sponsored By: QIAGEN
      2
      APR 21, 2021 | 12:00 AM
      High Throughput Testing for SARS-CoV-2 and SNP Analysis for Variants of Concern
      High Throughput Testing for SARS-CoV-2 and SNP Analysis for Variants...
      As part of Healthy Davis Together, we have implemented rapid, inexpensive, high throughput testing for SARS-Cov-2 using technology repurposed from the agricultural biotechnology sector. This...
      Speaker: Richard Michelmore
      Presented at: Genetics Week Virtual Event Series 2021
      1
      APR 20, 2021 | 7:30 AM
      C.E. CREDITS
      Keynote Presentation: A structural variation map of 499 Han Chinese individuals using long-read sequencing data
      Keynote Presentation: A structural variation map of 499 Han Chinese...
      Structural variants (SVs) are essential in human evolution and genetic disease but remain understudied. This is especially the case for non-Caucasian ethnicities. We report, for the first ti...
      Speaker: Fritz Sedlazeck, PhD
      Presented at: Genetics Week Virtual Event Series 2021
      1
      APR 20, 2021 | 12:00 AM
      C.E. CREDITS
      Telomere-to-Telomere Chromosome Assemblies: New Insights Into Genome Biology and Structure
      Telomere-to-Telomere Chromosome Assemblies: New Insights Into...
      We are entering into an exciting era of genomics where truly complete, high-quality assemblies of human chromosomes are available end-to-end, or from ‘telomere-to-telomere’ (T2T)...
      Speaker: Karen Miga, PhD
      Presented at: Genetics Week Virtual Event Series 2021
      2
      JAN 26, 2021 | 8:00 AM
      High-throughput CRISPR editing using the Onyx™ platform identifies essential residues in proteins
      High-throughput CRISPR editing using the Onyx™ platform identifies...
      Date: January 26, 2021 Time: 9:00am (PST), 12:00pm (EST) Over the past years, CRISPR/Cas genome editing has quickly become the method of choice for genetic manipulation, owing to its ease-of...
      Speaker: Liselot Dewachter, PhD , Laura Klitten, Ph.D.
      Sponsored By: Inscripta
      13 2
    JUN 21, 2022 | 6:00 AM
    The future of predictive genomics in Israel
    The future of predictive genomics in Israel
    Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
    Speaker: Zisis Kozlakidis , David Hanna , Dr. Yehudit Cohen , Sowmiya Moorthie , Shai Carmi
    Sponsored By: Thermo Fisher Scientific
    27
    MAY 17, 2022 | 8:00 AM
    Novel tools for cell line engineering with CRISPR-Cas9
    Novel tools for cell line engineering with CRISPR-Cas9
    Date: May 17, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) The CRISPR-Cas9 system provides researchers a powerful tool for manipulation of cells to create disease models, reporter c...
    Speaker: Louise Baskin, M.S. Molecular Biology and Genetics
    Sponsored By: Thermo Fisher Scientific
    13
    MAY 05, 2022 | 8:00 AM
    Solve Every Hereditary Disorder Case for All Types of Genetic Variation
    Solve Every Hereditary Disorder Case for All Types of Genetic...
    Date: May 5, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CET) Despite incredible advances in genomics, the NGS data interpretation workflow for hereditary diseases remains challenging...
    Speaker: Georgios Stamoulis, Ph.D.
    Sponsored By: QIAGEN
    7
    MAR 09, 2022 | 8:00 AM
    Biosecurity for Genome Engineering: Safety First and the SeqScreen Solution for Functional Predictions
    Biosecurity for Genome Engineering: Safety First and the SeqScreen...
    Date: March 09, 2021 Time: 08:00am (PST), 11:00am (EST), 5:00pm (CEST) Bioengineering technologies hold enormous potential to solve many of our world’s pressing problems. However, they...
    Speaker: Todd Treangen, PhD , Krista Ternus, PhD, PMP , Beth Vitalis, PhD
    Sponsored By: Inscripta
    5
    DEC 07, 2021 | 8:00 AM
    T Cells in COVID-19 - Translating research findings into clinical options
    T Cells in COVID-19 - Translating research findings into clinical...
    Date: November 7, 2021 Time: 8:00am (PDT), 11:00am (EDT) Analysis of SARS-CoV-2–specific T cells is important to understand their role during acute disease and in long-term protection...
    Speaker: Lorenz Fülle, PhD , Kirsten Langeveld
    Sponsored By: Miltenyi Biotec
    10 1
     
     
  • NOV 17, 2021 | 7:00 AM
    CRISPR-based transcriptional activation tools for silent genes in filamentous fungi
    CRISPR-based transcriptional activation tools for silent genes...
    DATE: November 17, 2021 TIME: 7:00am PST, 10:00am EST, 4:00pm CET Filamentous fungi are historically known to be a rich reservoir of bioactive compounds that are applied in a myriad of field...
    Speaker: Laszlo Mozsik
    Sponsored By: GenScript
    13 2
    NOV 08, 2021 | 9:00 AM
    Discovering Unforeseen Protein Assemblies with Mass Photometry
    Discovering Unforeseen Protein Assemblies with Mass Photometry
    Date: November 08, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CET) Many enzymes form homomeric complexes comprised of subunits encoded by the same gene. Assembly into homomeric...
    Speaker: Franziska Sendker
    Sponsored By: Refeyn
    4
    OCT 28, 2021 | 8:00 AM
    How to Advance Cell & Gene Therapies with an Innovative, Flexible Approach
    How to Advance Cell & Gene Therapies with an Innovative, Flexible...
    Date: October 28, 2021 Time: 8:00am (PDT), 11:00am (EDT) Today’s health industry is leveraging new, cutting-edge Cell & Gene Therapies (CGTs) to address a variety of medical illnes...
    Speaker: ​​​​​​​Chris ​Andrews , Domenico Palumberi
    Sponsored By: Dassault Systèmes
    6
    OCT 14, 2021 | 7:00 AM
    Heterologous Protein Engineering and characterization of native regulatory elements  in E. coli using Massively Parallel CRISPR Editing
    Heterologous Protein Engineering and characterization of native...
    Date: October 14, 2021 Time: 7:00am (PDT), 10:00am (EDT) Heterologous protein expression in model organisms has many applications, including protein engineering, production of industrial enz...
    Speaker: Tyson Shepherd, PhD
    Sponsored By: Inscripta
    22
    OCT 07, 2021 | 7:00 AM
    Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis
    Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common...
    DATE: October 7, 2021 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CET The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of...
    Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
    Sponsored By: Eppendorf, Promega
    12
     
     
  • SEP 29, 2021 | 9:00 AM
    Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome Engineering
    Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome...
    RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 and novel RNA targeti...
    Speaker: Omar Abudayyeh, PhD , Jonathan Gootenberg, PhD
    Presented at: CRISPR Virtual Event Series 2021
    1
    SEP 09, 2021 | 7:00 AM
    Maximize your variant knowledge using the Human Gene Mutation Database (HGMD®) Professional
    Maximize your variant knowledge using the Human Gene Mutation...
    Date: September 9, 2021 Time: 7:00am (PDT), 10:00am (EDT) Founded and maintained by the Institute of Medical Genetics at Cardiff University in 1996, HGMD attempts to collate all known, publi...
    Speaker: Dr. Ruth Burton
    Sponsored By: QIAGEN
    3
    SEP 08, 2021 | 12:00 AM
    SeXX Matters for Respiratory Viral Pathogenesis and Responses to Vaccines
    SeXX Matters for Respiratory Viral Pathogenesis and Responses...
    The ongoing COVID-19 pandemic has increased awareness about sex-specific differences in immunity and outcomes following respiratory virus infections. Strong evidence of a male bias in COVID-...
    Speaker: Sabra L. Klein, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    0
    SEP 07, 2021 | 12:00 AM
    CMPK2 Restricts the Replication of Multiple Flaviviruses
    CMPK2 Restricts the Replication of Multiple Flaviviruses
    Flaviviruses are pathogens of global public health concern. They include dengue virus (DENV), West Nile virus (WNV), and zika virus (ZIKV). There are no approved US Food and Drug Administrat...
    Speaker: Maudry Laurent-Rolle, MD/PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    2
    SEP 07, 2021 | 12:00 AM
    Immune-Mediated Pathologies During Dengue Virus Infection
    Immune-Mediated Pathologies During Dengue Virus Infection
    Flaviviruses are pathogens of global public health concern. They include dengue virus (DENV), West Nile virus (WNV), and zika virus (ZIKV). There are no approved US Food and Drug Administrat...
    Speaker: Ashley St. John, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    1
     
     
  • AUG 25, 2021 | 12:00 AM
    Alpha-synuclein Aggregation in Parkinson's Disease - Is it Relevant and How can we Model it?
    Alpha-synuclein Aggregation in Parkinson's Disease - Is it Relevant...
    Parkinson’s disease (PD) is the second most common neurodegenerative disorder with multiple motor and non-motor symptoms. PD is characterized by the presence on proteinaceous neuronal...
    Speaker: Andrii Domanskyi, PhD
    Presented at: Neuroscience Virtual Event Series 2021
    5
    JUL 22, 2021 | 2:45 PM
    Library-selected AAV variants can effectively translate to non-human primates in the spinal cord and cochlea
    Library-selected AAV variants can effectively translate to non-human...
    AAV peptide display libraries allow for the generation of novel variants capable of high-level transduction. We previously described an AAV peptide display library combined with a sensitive...
    Speaker: Killian S. Hanlon, PhD
    Presented at: GenScript Gene & Cell Engineering Virtual Summit
    1
    JUL 13, 2021 | 11:00 AM
    Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow
    Monoclonality Assurance Using CloneSelect Single-Cell Printer...
    Date: July 13, 2021 Time: 11:00am (PDT), 1:00pm (EDT) 1 R&D, Cell Culture & Fermentation Sciences, Bio Pharmaceutical Development, AstraZeneca, Gaithersburg, USA, 2 R&D, ADPE, Ce...
    Speaker: Gargi Roy
    Sponsored By: Molecular Devices
    9 10
    MAY 11, 2021 | 1:30 PM
    The Lifecycle of Communicating the Science panel
    The Lifecycle of Communicating the Science panel
    ...
    Speaker: Thomas F. Babor, PhD, MPH , May Berenbaum, PhD , Holly Falk-Krzesinski, PhD , Moira Gunn, PhD , Jessica Miles, PhD
    Presented at: 5th Annual Vivian Pinn Symposium
    2
    APR 22, 2021 | 6:00 AM
    Panel Presentation: Powering the precision in "precision medicine" - The role of curated data, databases and knowledge: Does a gold standard exist?
    Panel Presentation: Powering the precision in "precision medicine"...
    As NGS panels grow, test interpretation becomes more complex and time-consuming / With the explosion of NGS data, the need for consolidated databases with reliable and transparent curation a...
    Speaker: Dominic John , Simon Forbes, PhD , Shawn Bauer , Lisa Barrow-Laing
    Presented at: Genetics Week Virtual Event Series 2021
    Sponsored By: QIAGEN
    2
     
     
  • APR 21, 2021 | 12:00 AM
    High Throughput Testing for SARS-CoV-2 and SNP Analysis for Variants of Concern
    High Throughput Testing for SARS-CoV-2 and SNP Analysis for Variants...
    As part of Healthy Davis Together, we have implemented rapid, inexpensive, high throughput testing for SARS-Cov-2 using technology repurposed from the agricultural biotechnology sector. This...
    Speaker: Richard Michelmore
    Presented at: Genetics Week Virtual Event Series 2021
    1
    APR 20, 2021 | 7:30 AM
    C.E. CREDITS
    Keynote Presentation: A structural variation map of 499 Han Chinese individuals using long-read sequencing data
    Keynote Presentation: A structural variation map of 499 Han Chinese...
    Structural variants (SVs) are essential in human evolution and genetic disease but remain understudied. This is especially the case for non-Caucasian ethnicities. We report, for the first ti...
    Speaker: Fritz Sedlazeck, PhD
    Presented at: Genetics Week Virtual Event Series 2021
    1
    APR 20, 2021 | 12:00 AM
    C.E. CREDITS
    Telomere-to-Telomere Chromosome Assemblies: New Insights Into Genome Biology and Structure
    Telomere-to-Telomere Chromosome Assemblies: New Insights Into...
    We are entering into an exciting era of genomics where truly complete, high-quality assemblies of human chromosomes are available end-to-end, or from ‘telomere-to-telomere’ (T2T)...
    Speaker: Karen Miga, PhD
    Presented at: Genetics Week Virtual Event Series 2021
    2
    JAN 26, 2021 | 8:00 AM
    High-throughput CRISPR editing using the Onyx™ platform identifies essential residues in proteins
    High-throughput CRISPR editing using the Onyx™ platform identifies...
    Date: January 26, 2021 Time: 9:00am (PST), 12:00pm (EST) Over the past years, CRISPR/Cas genome editing has quickly become the method of choice for genetic manipulation, owing to its ease-of...
    Speaker: Liselot Dewachter, PhD , Laura Klitten, Ph.D.
    Sponsored By: Inscripta
    13 2
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