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    Translational Oncology

    Translational Oncology involves moving scientific discoveries made in a laboratory setting into clinical applications that enhance treatment efficacy and outcomes for cancer patients. Translational Oncology approaches include pre-clinical research, which can be done in an in vivo, in vitro, or ex vivo setting, before validating the laboratory findings with a clinical trial.

    Webinars

    • OCT 08, 2020 | 7:30 AM
      Keynote Presentation: RNA Splicing and Ancestry-related Molecular Targets in Precision Oncology and Cancer Disparities
      Keynote Presentation: RNA Splicing and Ancestry-related Molecular...
      Individuals of under-represented minority ancestry are at disproportional risk for higher incidence and mortality rates for particular cancers. The unequal burden of cancer in certain racial...
      Speaker: Steven R. Patierno, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
      4
      OCT 08, 2020 | 7:00 AM
      Protect your cells with proper pipetting - How liquid handling influences your cell culture work
      Protect your cells with proper pipetting - How liquid handling...
      DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
      Speaker: Nadine Mellies, PhD , Jessica Wagener, PhD
      Sponsored By: Eppendorf
      33 5
      OCT 07, 2020 | 10:30 AM
      Immune correlates of TP53 mutational status in acute myeloid leukemia
      Immune correlates of TP53 mutational status in acute myeloid leukemia
      Tumor phenotypes are dictated not only by the neoplastic cell component, but also by the tumor microenvironment (TME), which is inherently immuno-suppressive, is equipped to hamper effector...
      Speaker: Dr. Sergio Rutella
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
      Sponsored By: NanoString Technologies
      1
      OCT 07, 2020 | 6:00 AM
      Keynote Presentation: Robotic Radical Prostatectomy:  Lessons learned from 14,000 cases
      Keynote Presentation: Robotic Radical Prostatectomy: Lessons...
      This lecture will encompass the learning experience I have had over my 14,000 RALP cases. We will present an evidence based prospective data collection and analysis of RALP outcomes. We will...
      Speaker: Vipul Patel, MD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
      1
      OCT 07, 2020 | 12:00 AM
      Immunotherapy in Prostate and Renal Cell Carcinoma: Panoply of Futuristic Therapies
      Immunotherapy in Prostate and Renal Cell Carcinoma: Panoply of...
      Immune therapy approaches are being used in many different solid cancers today including clear cell renal carcinoma (mccRCC) and metastatic prostate adenocarcinoma. Over the past few years i...
      Speaker: Benjamin Maughan, MD, PharmD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
      4
      SEP 23, 2020 | 4:00 PM
      Break-out: New Applications for CyTOF® systems and Imaging Mass Cytometry™ from Fluidigm
      Break-out: New Applications for CyTOF® systems and Imaging Mass...
      Live-cell barcoding with Cd-CD45 antibodies 4:00–4:30 pm PDT Michelle Poulin, PhD Creating CyTOF panels just got even easier: Panel Designer 2.0 4:30–5:00 pm PDT Kevin Brown, PhD...
      Speaker: Michelle Poulin, PhD , Kevin Brown, PhD , Noah Saederup, PhD , Andrew Quong, PhD
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      1
      SEP 23, 2020 | 12:00 PM
      Mass Cytometry in Translational Research: Session II
      Mass Cytometry in Translational Research: Session II
      B cells and tertiary lymphoid structures (TLS) promote immunotherapy response 1:00–1:30 pm PDT Presented By: Rafet Basar, MD...
      Speaker: Handan Xiang, PhD , Pei-Yu Chen, PhD , Hiranmayi Ravichandran, MS , Rafet Basar, MD , Amir Horowitz, PhD
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      2
      SEP 23, 2020 | 11:30 AM
      Break-out: Setting Yourself Up for Core Success - A panel discussion
      Break-out: Setting Yourself Up for Core Success - A panel discussion
      Suspension by day, imaging by night: how to efficiently manage your system and optimize utilization Presented By: Jared K. Burks, PhD Mass cytometry, be it suspension or imaging, opens the d...
      Speaker: Jared K. Burks, PhD , Matthew Cochran, MS , Emily Thrash, PhD , Akil Merchant, MD
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      1
      SEP 23, 2020 | 6:30 AM
      Mass Cytometry in Translational Research: Session I
      Mass Cytometry in Translational Research: Session I
      Single-cell mass cytometry identifies mechanisms of resistance to immunotherapy in AML 6:30–7:00 am PDT Presented By: Shelley Herbrich, PhD Understanding CD19 negative relapse followin...
      Speaker: Shelley Herbrich, PhD , Kara L. Davis, DO , Bernd Bodenmiller, PhD
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      2
      SEP 23, 2020 | 6:05 AM
      Break-out: New Applications for CyTOF® systems and Imaging Mass Cytometry™ from Fluidigm
      Break-out: New Applications for CyTOF® systems and Imaging Mass...
      Live-cell barcoding with Cd-CD45 antibodies 6:00–6:30 am PDT Presented By: Michelle Poulin, PhD Creating CyTOF® panels just got even easier: Panel Designer 2.0 6:30–7:00 am P...
      Speaker: Michelle Poulin, PhD , Kevin Brown, PhD , Noah Saederup, PhD , Andrew Quong, PhD
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      2
      SEP 23, 2020 | 6:00 AM
      Welcome and Opening Remarks
      Welcome and Opening Remarks
      ...
      Speaker: Chris Linthwaite , Andrew Quong, PhD , Alex Cherkassky, PhD
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      1
      AUG 06, 2020 | 10:00 AM
      Part VII: From biochemical nanosensors to imaging to informatics to COVID-19 convalescent plasma - developing diagnostics and therapies for clinical medicine
      Part VII: From biochemical nanosensors to imaging to informatics...
      Current cancer management follows a multipronged approach that include surgery, radiation, and chemotherapy. There is a pressing need for a platform technology to provide precision chemother...
      Speaker: Freddy Nguyen, MD, PhD
      Presented at: 2020 Beckman Symposium
      1
      AUG 06, 2020 | 12:00 AM
      From biochemical nanosensors to imaging to informatics to COVID-19 convalescent plasma - developing diagnostics and therapies for clinical medicine
      From biochemical nanosensors to imaging to informatics to COVID-19...
      Current cancer management follows a multipronged approach that include surgery, radiation, and chemotherapy. There is a pressing need for a platform technology to provide precision chemother...
      Speaker: Freddy Nguyen, MD, PhD
      Presented at: 2020 Beckman Symposium
      1
      JUL 29, 2020 | 2:00 PM
      Route of 41BB/41BBL costimulation determines effector function of B7-H3-CAR.CD28ζ T cells
      Route of 41BB/41BBL costimulation determines effector function...
      B7-H3 is actively being explored as an immunotherapy target for pediatric patients with solid tumors using monoclonal antibodies or T cells expressing chimeric antigen receptors (CARs). B7-H...
      Speaker: Christopher DeRenzo, MD
      Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
      1
      JUL 29, 2020 | 1:00 PM
      Using quantitative imaging microscopy to explore and target the molecular origins of cancer
      Using quantitative imaging microscopy to explore and target the...
      The identification of novel drug targets and the development of next generation therapeutic strategies remain elusive goals for cancer researchers. We believe that the aberrant molecular eve...
      Speaker: Kirk McManus, PhD
      Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
      1
      JUL 29, 2020 | 8:00 AM
      High-throughput DNA damage analyses identify synergistic drug combinations to treat malignant brain tumours
      High-throughput DNA damage analyses identify synergistic drug...
      Current methods to treat malignant brain tumors are highly intrusive, leading to poor quality of life with abysmal patient survival rates. Combined with radiation therapy, first/second-line...
      Speaker: Sachin Katyal, PhD
      Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
      1
      JUL 29, 2020 | 12:00 AM
      Modeling anti-tumor function of human T cells
      Modeling anti-tumor function of human T cells
      Mouse models have been essential for establishing the role of the T cells in anti-tumor responses, but they have limited utility in translational studies for the testing of candidate therape...
      Speaker: Michael Overstreet, PhD
      Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
      1
      JUL 07, 2020 | 9:30 AM
      Evaluating tumor-immune cell interactions in human lung cancer using multiparametric and spatially resolved tissue analysis
      Evaluating tumor-immune cell interactions in human lung cancer...
      ...
      Speaker: Kurt Schalper, MD, PhD
      Presented at: Fluidigm Infectious Disease and Oncology Virtual Summit
      1
      JUL 02, 2020 | 12:45 PM
      Cancer genomics research on tumor clonality and liquid biopsy
      Cancer genomics research on tumor clonality and liquid biopsy
      Most bladder cancer (BC) cases are non-muscle-invasive BC (NIBMC), which is rarely lethal. However, nearly one-fifth of NMIBC cases progress to muscle-invasive BC (MIBC), which shows a 5-yea...
      Speaker: Yosuke Hirotsu, PhD
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      1
      JUL 02, 2020 | 12:15 PM
      Microsatellite instability analysis for studying immunotherapy and Lynch syndrome-related cancers
      Microsatellite instability analysis for studying immunotherapy...
      Microsatellite instability (MSI) is a hypermutation phenotype that results from impaired DNA mismatch repair (MMR). The presence of high levels of MSI (MSI-H) is potentially predictive of Ly...
      Speaker: Charles Baudo, PhD
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      1
      JUN 30, 2020 | 10:00 AM
      COVID-19 Serology Assays and Cell Therapy Strategies
      COVID-19 Serology Assays and Cell Therapy Strategies
      DATE: June 30, 2020 TIME: 10:00 am PDT, 1:00 pm EDT COVID-19 is a highly pathogenic disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We have assembled a panel...
      Speaker: Fatima Amanat , Dean Lee , Lior Raviv
      Sponsored By: Thermo Fisher Scientific/Gibco
      30 1
      JUN 30, 2020 | 9:00 AM
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
      DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
      Speaker: Jane Weitzel
      Sponsored By: Sartorius
      17 1
      JUN 30, 2020 | 8:00 AM
      Phenotyping the Tumor Microenvironment Using DNA Barcoded Multiplex Technology
      Phenotyping the Tumor Microenvironment Using DNA Barcoded Multiplex...
      DATE: June 30, 2020 TIME: 8:00am PT, 11:00am Understanding the tumor microenvironment (TME) is essential for the development of effective treatments for cancer patients. Traditional multi...
      Speaker: Chifei Sun
      Sponsored By: Leica Biosystems
      19
      JUN 17, 2020 | 3:00 PM
      COVID-19: Enrichment and analysis of rare antigen- and virus-specific T cells
      COVID-19: Enrichment and analysis of rare antigen- and virus-specific...
      Analysis of antigen- and virus-specific T cells is essential to the understanding of fundamental immunological processes in the contexts of e.g. infectious diseases, immuno-oncology, as well...
      Speaker: Dr. Marcello Stein
      Presented at: Coronavirus Series
      Sponsored By: Miltenyi Biotec
      1
    OCT 08, 2020 | 7:30 AM
    Keynote Presentation: RNA Splicing and Ancestry-related Molecular Targets in Precision Oncology and Cancer Disparities
    Keynote Presentation: RNA Splicing and Ancestry-related Molecular...
    Individuals of under-represented minority ancestry are at disproportional risk for higher incidence and mortality rates for particular cancers. The unequal burden of cancer in certain racial...
    Speaker: Steven R. Patierno, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
    4
    OCT 08, 2020 | 7:00 AM
    Protect your cells with proper pipetting - How liquid handling influences your cell culture work
    Protect your cells with proper pipetting - How liquid handling...
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    Speaker: Nadine Mellies, PhD , Jessica Wagener, PhD
    Sponsored By: Eppendorf
    33 5
    OCT 07, 2020 | 10:30 AM
    Immune correlates of TP53 mutational status in acute myeloid leukemia
    Immune correlates of TP53 mutational status in acute myeloid leukemia
    Tumor phenotypes are dictated not only by the neoplastic cell component, but also by the tumor microenvironment (TME), which is inherently immuno-suppressive, is equipped to hamper effector...
    Speaker: Dr. Sergio Rutella
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
    Sponsored By: NanoString Technologies
    1
    OCT 07, 2020 | 6:00 AM
    Keynote Presentation: Robotic Radical Prostatectomy:  Lessons learned from 14,000 cases
    Keynote Presentation: Robotic Radical Prostatectomy: Lessons...
    This lecture will encompass the learning experience I have had over my 14,000 RALP cases. We will present an evidence based prospective data collection and analysis of RALP outcomes. We will...
    Speaker: Vipul Patel, MD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
    1
    OCT 07, 2020 | 12:00 AM
    Immunotherapy in Prostate and Renal Cell Carcinoma: Panoply of Futuristic Therapies
    Immunotherapy in Prostate and Renal Cell Carcinoma: Panoply of...
    Immune therapy approaches are being used in many different solid cancers today including clear cell renal carcinoma (mccRCC) and metastatic prostate adenocarcinoma. Over the past few years i...
    Speaker: Benjamin Maughan, MD, PharmD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
    4
     
     
  • SEP 23, 2020 | 4:00 PM
    Break-out: New Applications for CyTOF® systems and Imaging Mass Cytometry™ from Fluidigm
    Break-out: New Applications for CyTOF® systems and Imaging Mass...
    Live-cell barcoding with Cd-CD45 antibodies 4:00–4:30 pm PDT Michelle Poulin, PhD Creating CyTOF panels just got even easier: Panel Designer 2.0 4:30–5:00 pm PDT Kevin Brown, PhD...
    Speaker: Michelle Poulin, PhD , Kevin Brown, PhD , Noah Saederup, PhD , Andrew Quong, PhD
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    1
    SEP 23, 2020 | 12:00 PM
    Mass Cytometry in Translational Research: Session II
    Mass Cytometry in Translational Research: Session II
    B cells and tertiary lymphoid structures (TLS) promote immunotherapy response 1:00–1:30 pm PDT Presented By: Rafet Basar, MD...
    Speaker: Handan Xiang, PhD , Pei-Yu Chen, PhD , Hiranmayi Ravichandran, MS , Rafet Basar, MD , Amir Horowitz, PhD
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    2
    SEP 23, 2020 | 11:30 AM
    Break-out: Setting Yourself Up for Core Success - A panel discussion
    Break-out: Setting Yourself Up for Core Success - A panel discussion
    Suspension by day, imaging by night: how to efficiently manage your system and optimize utilization Presented By: Jared K. Burks, PhD Mass cytometry, be it suspension or imaging, opens the d...
    Speaker: Jared K. Burks, PhD , Matthew Cochran, MS , Emily Thrash, PhD , Akil Merchant, MD
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    1
    SEP 23, 2020 | 6:30 AM
    Mass Cytometry in Translational Research: Session I
    Mass Cytometry in Translational Research: Session I
    Single-cell mass cytometry identifies mechanisms of resistance to immunotherapy in AML 6:30–7:00 am PDT Presented By: Shelley Herbrich, PhD Understanding CD19 negative relapse followin...
    Speaker: Shelley Herbrich, PhD , Kara L. Davis, DO , Bernd Bodenmiller, PhD
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    2
    SEP 23, 2020 | 6:05 AM
    Break-out: New Applications for CyTOF® systems and Imaging Mass Cytometry™ from Fluidigm
    Break-out: New Applications for CyTOF® systems and Imaging Mass...
    Live-cell barcoding with Cd-CD45 antibodies 6:00–6:30 am PDT Presented By: Michelle Poulin, PhD Creating CyTOF® panels just got even easier: Panel Designer 2.0 6:30–7:00 am P...
    Speaker: Michelle Poulin, PhD , Kevin Brown, PhD , Noah Saederup, PhD , Andrew Quong, PhD
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    2
     
     
  • SEP 23, 2020 | 6:00 AM
    Welcome and Opening Remarks
    Welcome and Opening Remarks
    ...
    Speaker: Chris Linthwaite , Andrew Quong, PhD , Alex Cherkassky, PhD
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    1
    AUG 06, 2020 | 10:00 AM
    Part VII: From biochemical nanosensors to imaging to informatics to COVID-19 convalescent plasma - developing diagnostics and therapies for clinical medicine
    Part VII: From biochemical nanosensors to imaging to informatics...
    Current cancer management follows a multipronged approach that include surgery, radiation, and chemotherapy. There is a pressing need for a platform technology to provide precision chemother...
    Speaker: Freddy Nguyen, MD, PhD
    Presented at: 2020 Beckman Symposium
    1
    AUG 06, 2020 | 12:00 AM
    From biochemical nanosensors to imaging to informatics to COVID-19 convalescent plasma - developing diagnostics and therapies for clinical medicine
    From biochemical nanosensors to imaging to informatics to COVID-19...
    Current cancer management follows a multipronged approach that include surgery, radiation, and chemotherapy. There is a pressing need for a platform technology to provide precision chemother...
    Speaker: Freddy Nguyen, MD, PhD
    Presented at: 2020 Beckman Symposium
    1
    JUL 29, 2020 | 2:00 PM
    Route of 41BB/41BBL costimulation determines effector function of B7-H3-CAR.CD28ζ T cells
    Route of 41BB/41BBL costimulation determines effector function...
    B7-H3 is actively being explored as an immunotherapy target for pediatric patients with solid tumors using monoclonal antibodies or T cells expressing chimeric antigen receptors (CARs). B7-H...
    Speaker: Christopher DeRenzo, MD
    Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
    1
    JUL 29, 2020 | 1:00 PM
    Using quantitative imaging microscopy to explore and target the molecular origins of cancer
    Using quantitative imaging microscopy to explore and target the...
    The identification of novel drug targets and the development of next generation therapeutic strategies remain elusive goals for cancer researchers. We believe that the aberrant molecular eve...
    Speaker: Kirk McManus, PhD
    Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
    1
     
     
  • JUL 29, 2020 | 8:00 AM
    High-throughput DNA damage analyses identify synergistic drug combinations to treat malignant brain tumours
    High-throughput DNA damage analyses identify synergistic drug...
    Current methods to treat malignant brain tumors are highly intrusive, leading to poor quality of life with abysmal patient survival rates. Combined with radiation therapy, first/second-line...
    Speaker: Sachin Katyal, PhD
    Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
    1
    JUL 29, 2020 | 12:00 AM
    Modeling anti-tumor function of human T cells
    Modeling anti-tumor function of human T cells
    Mouse models have been essential for establishing the role of the T cells in anti-tumor responses, but they have limited utility in translational studies for the testing of candidate therape...
    Speaker: Michael Overstreet, PhD
    Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
    1
    JUL 07, 2020 | 9:30 AM
    Evaluating tumor-immune cell interactions in human lung cancer using multiparametric and spatially resolved tissue analysis
    Evaluating tumor-immune cell interactions in human lung cancer...
    ...
    Speaker: Kurt Schalper, MD, PhD
    Presented at: Fluidigm Infectious Disease and Oncology Virtual Summit
    1
    JUL 02, 2020 | 12:45 PM
    Cancer genomics research on tumor clonality and liquid biopsy
    Cancer genomics research on tumor clonality and liquid biopsy
    Most bladder cancer (BC) cases are non-muscle-invasive BC (NIBMC), which is rarely lethal. However, nearly one-fifth of NMIBC cases progress to muscle-invasive BC (MIBC), which shows a 5-yea...
    Speaker: Yosuke Hirotsu, PhD
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    1
    JUL 02, 2020 | 12:15 PM
    Microsatellite instability analysis for studying immunotherapy and Lynch syndrome-related cancers
    Microsatellite instability analysis for studying immunotherapy...
    Microsatellite instability (MSI) is a hypermutation phenotype that results from impaired DNA mismatch repair (MMR). The presence of high levels of MSI (MSI-H) is potentially predictive of Ly...
    Speaker: Charles Baudo, PhD
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    1
     
     
  • JUN 30, 2020 | 10:00 AM
    COVID-19 Serology Assays and Cell Therapy Strategies
    COVID-19 Serology Assays and Cell Therapy Strategies
    DATE: June 30, 2020 TIME: 10:00 am PDT, 1:00 pm EDT COVID-19 is a highly pathogenic disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We have assembled a panel...
    Speaker: Fatima Amanat , Dean Lee , Lior Raviv
    Sponsored By: Thermo Fisher Scientific/Gibco
    30 1
    JUN 30, 2020 | 9:00 AM
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
    DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
    Speaker: Jane Weitzel
    Sponsored By: Sartorius
    17 1
    JUN 30, 2020 | 8:00 AM
    Phenotyping the Tumor Microenvironment Using DNA Barcoded Multiplex Technology
    Phenotyping the Tumor Microenvironment Using DNA Barcoded Multiplex...
    DATE: June 30, 2020 TIME: 8:00am PT, 11:00am Understanding the tumor microenvironment (TME) is essential for the development of effective treatments for cancer patients. Traditional multi...
    Speaker: Chifei Sun
    Sponsored By: Leica Biosystems
    19
    JUN 17, 2020 | 3:00 PM
    COVID-19: Enrichment and analysis of rare antigen- and virus-specific T cells
    COVID-19: Enrichment and analysis of rare antigen- and virus-specific...
    Analysis of antigen- and virus-specific T cells is essential to the understanding of fundamental immunological processes in the contexts of e.g. infectious diseases, immuno-oncology, as well...
    Speaker: Dr. Marcello Stein
    Presented at: Coronavirus Series
    Sponsored By: Miltenyi Biotec
    1
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