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    Viral

    The term viral refers to an infection driven by a virus and examples include COVID-19, AIDS, measles, hepatitis, and smallpox. Viruses contain DNA or RNA and must infect host cells in order to replicate and they can damage the host during this process. Antiviral medications or vaccines can treat viral infections.

    Webinars

    • APR 07, 2021 | 12:00 AM
      Model-based Control for Continuous Viral Inactivation of Biopharmaceuticals
      Model-based Control for Continuous Viral Inactivation of Biopharmaceuticals
      Biologics derived from mammalian sources are expected to undergo two orthogonal virus removal processes. One common processing step is a batch low-pH hold to inactivate enveloped viruses. At...
      Speaker: Moo Sun Hong, MS
      Presented at: Bioprocessing Virtual Event Series 2021
      3 1
      APR 06, 2021 | 10:00 AM
      Invitrogen iBright Analysis Software - an introduction and overview of key analysis workflows
      Invitrogen iBright Analysis Software - an introduction and overview...
      DATE: April 6, 2021 TIME: 10:00am PDT Accurate and efficient data analysis of protein targets is an integral part of the gel and western blot imaging workflow. The Invitrogen iBright Analysi...
      Speaker: Janaki Narahari, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      1
      APR 01, 2021 | 8:00 AM
      Develop High Quality Therapeutic Antibodies Straight From Selections in Record Time
      Develop High Quality Therapeutic Antibodies Straight From Selections...
      Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
      Speaker: Andrew Bradbury, MD, PhD , Noah Ditto
      Sponsored By: Carterra
      3 1
      MAR 30, 2021 | 8:00 AM
      Utilizing the Gibcoā„¢ CTSā„¢ Roteaā„¢ System, a versatile closed manufacturing system, for cell and gene therapy development
      Utilizing the Gibcoā„¢ CTSā„¢ Roteaā„¢ System, a versatile closed manufacturing...
      DATE: March 30, 2021 TIME: 08:00am PST In this talk, we will discuss the value of moving towards modular and automated, closed-system technologies designed to enable scalable and cost-effect...
      Speaker: Mary Ann Santos , Emily Benz , Eric Dubofsky
      Sponsored By: Thermo Fisher Scientific/Gibco
      6
      MAR 25, 2021 | 8:00 AM
      Expanding ERF (Equivalent Number of Reference Fluorophore) Value Assignments for Quantitative Flow Cytometry- More Colors and Smaller Particles
      Expanding ERF (Equivalent Number of Reference Fluorophore) Value...
      Date: March 25, 2021 Time: 8:00am (PST), 11:00am (EST) Flow cytometry has expanded to have more users, more data, and multiple instrument types. While this has lead to more quantitative data...
      Speaker: Paul DeRose
      Sponsored By: Thermo Fisher Scientific
      12
      MAR 22, 2021 | 8:00 AM
      Impact of Global Surveillance on Diagnostic Tools
      Impact of Global Surveillance on Diagnostic Tools
      Date: March 22, 2021 Time: 8:00am PDT, 11:00am EDT Viral diversity presents an ongoing challenge for diagnostic tests, which must be designed to accurately detect all current and future circ...
      Speaker: Dr. Gavin Cloherty
      Sponsored By: Scientific Affairs, Molecular Diagnostics at Abbott
      4 1
      MAR 16, 2021 | 8:00 AM
      The latest developments for enriching and analyzing antigen- and virus-specific T cells
      The latest developments for enriching and analyzing antigen- and...
      DATE: March 09, 2021 TIME: 08:00am PST The COVID-19 pandemic has placed urgency on precise analysis of rare antigen- and virus-specific T cells. At Miltenyi Biotec, we offer a complete workf...
      Speaker: Lorenz Fülle, PhD
      Sponsored By: Miltenyi Biotec
      12
      FEB 24, 2021 | 10:00 AM
      Biosafety Enclosures and the Automated Lab: Steps for Success
      Biosafety Enclosures and the Automated Lab: Steps for Success
      DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
      Speaker: David Wasescha , Simon Fogarty
      Sponsored By: Labconco
      15
      FEB 24, 2021 | 12:00 AM
      Evolution of a Platform-Based Non-Clinical Safety Assessment Approach for Vaccines
      Evolution of a Platform-Based Non-Clinical Safety Assessment Approach...
      Platform based therapeutics and vaccines offer a unique opportunity to simplify and streamline the drug development process. Moderna has taken the approach of harnessing the power of mRNA as...
      Speaker: Joseph J. Senn, PhD, DABT
      Presented at: Drug Discovery & Development Virtual Event Series 2021
      2
      FEB 11, 2021 | 8:00 AM
      Keep it small! AAV & LNP quant and sizing from just microliters
      Keep it small! AAV & LNP quant and sizing from just microliters
      Date: February 11, 2021 Time: 8:00am (PST), 11:00am (EST) Getting answers on what’s in your precious AAV, LVV, or lipid nanoparticle (LNP) sample means wanting to use the lowest possib...
      Speaker: Nelis Denys
      Sponsored By: Unchained Labs
      2
      FEB 10, 2021 | 7:00 AM
      Influenza vaccine viral protein profiling for viral potency
      Influenza vaccine viral protein profiling for viral potency
      Date: February 10, 2021 Time: 7:00am (PST), 10:00 am (EST) In this talk today we will discuss a new approach to profile influenza proteins. Influenza vaccines typically consist of either a...
      Speaker: Stephen Lock, PhD , Patrick Buckley, PhD
      Sponsored By: SCIEX
      17
      JAN 21, 2021 | 10:00 AM
      Applications of Procalcitonin-aided Decision Making for Antibiotic Stewardship in Emergency Care
      Applications of Procalcitonin-aided Decision Making for Antibiotic...
      Date: January 21, 2021 Time: 10:00am (PST) Abstract Learning Objectives Discuss Procalcitonin as a biomarker to help distinguish viral versus bacterial infection Review the literature suppor...
      Speaker: Larissa May
      Sponsored By: Thermo Fisher Scientific
      9 1
      JAN 21, 2021 | 8:00 AM
      Critical reagent characterization for regulated bioassays accelerated by Uncle
      Critical reagent characterization for regulated bioassays accelerated...
      Date: January 21, 2021 Time: 8:00am (PST), 11:00am (EST) Today, critical reagent characterization is a key component in the overall workflow to establish robust ligand binding assays (e.g.,...
      Speaker: Ron Bowsher , Adam Kinne , Kevin Lance, Ph.D.
      Sponsored By: Unchained Labs
      6
      JAN 14, 2021 | 7:00 AM
      Cell Culture Bioprocess Scale-Up Workflow from Bench to Pilot/Production Scale using Single-Use Bioreactors
      Cell Culture Bioprocess Scale-Up Workflow from Bench to Pilot/Production...
      DATE: January 14, 2021 TIME: 7:00am PST Transferring a biologic candidate from the research and development phase to commercial production usually requires increasing the working volume...
      Speaker: Amanda Suttle , Dr. Ma Sha , Rich Mirro
      Presented at: Eppendorf Planet of Knowledge - A New Dimension
      Sponsored By: Eppendorf
      16 1
      DEC 18, 2020 | 5:00 AM
      A new approach of COVID-19 surveillance testing to maximize the identification of asymptomatic patients
      A new approach of COVID-19 surveillance testing to maximize the...
      Date: December 18, 2020 Time: 2:00pm PST Europe continues to battle a second wave of COVID19 epidemic; more than 30.000 newly infected patients are being confirmed daily in Italy. As of Nove...
      Speaker: Vittorio Sambri
      Sponsored By: Diasorin
      20 1
      DEC 17, 2020 | 9:00 AM
      Advancing Cell Biology with Cryo-Correlative Microscopy
      Advancing Cell Biology with Cryo-Correlative Microscopy
      Date: December 17, 2020 Time: 9:00am (PST), 12:00pm (EST) Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging moda...
      Speaker: Elizabeth Wright, Ph.D. , Jae Yang, Ph.D. , Bryan Sibert, Ph.D. , Joseph Kim
      Sponsored By: Leica Microsystems, Alveole
      13 2
      DEC 15, 2020 | 10:00 AM
      Prep for Success: From human microbiome exploration to advanced SARS-CoV-2 testing in wastewater
      Prep for Success: From human microbiome exploration to advanced...
      DATE: December 15, 2020 TIME: 10:00am PST Scientists from Thermo Fisher Scientific will walk us through the world of microorganisms. They will discuss their most recent research on viruses,...
      Speaker: Sasha Vlassov, PhD , Jasti Madhu , Emily Zeringer
      Sponsored By: Thermo Fisher Scientific
      13
      DEC 04, 2020 | 9:00 AM
      IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody Cocktail - Visualizing immune response during infections
      IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody...
      Date: December 4, 2020 Time: 9:00am PST, 12:00pm EST Activation of Myeloid cells and interferon release is an early response to infection. The 3-marker combination (CD169/CD64/HLA-DR) gives...
      Speaker: Shankar Pattabhiraman
      Sponsored By: Beckman Coulter Life Sciences
      5
      DEC 03, 2020 | 4:30 PM
      An Iterative Approach to Structure-based in silico to in vivo Development of Combinatorial Anti-SARS-CoV-2 Therapy
      An Iterative Approach to Structure-based in silico to in vivo...
      This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
      Speaker: Garth Ehrlich, PhD, FAAAS
      Presented at: Coronavirus Series
      5
      DEC 03, 2020 | 3:00 PM
      Novel SARS-Cov-2 Neutralization Antibody test, performance and applications
      Novel SARS-Cov-2 Neutralization Antibody test, performance and...
      The presentation will provide a review of major emerging zoonotic virus outbreaks from Hendra virus in 1994 in Australia to SARS-CoV-2 in China 2019 and our latest findings on bat biology an...
      Speaker: Roumen Bogoev, MSc
      Presented at: Coronavirus Series
      Sponsored By: GenScript
      3
      DEC 03, 2020 | 12:00 PM
      Advancing SARS-CoV-2 Diagnostics Through Standardization and Quantitation
      Advancing SARS-CoV-2 Diagnostics Through Standardization and Quantitation
      The COVID-19 pandemic continues to have a grip on worldwide health and global economics. Advancing molecular diagnostics is central to the diagnosis and prevention efforts surrounding the de...
      Speaker: Russell Garlick, PhD , Marc Salit, PhD
      Presented at: Coronavirus Series
      Sponsored By: LGC Seracare
      2
      DEC 03, 2020 | 9:00 AM
      New! PerkinElmerĀ® RT-PCR SARS-CoV-2 Solution Updates
      New! PerkinElmerĀ® RT-PCR SARS-CoV-2 Solution Updates
      To execute COVID-19 testing strategies effectively, labs need to procure reliable components of the workflow without interruption – from reagents to equipment and consumables at the sc...
      Speaker: Arvind Kothandaraman
      Presented at: Coronavirus Series
      Sponsored By: PerkinElmer
      3
      DEC 03, 2020 | 7:30 AM
      Keynote Presentation: Prevalence of SARS-CoV-2 Immunity in Those Undiagnosed with COVID-19 in the U.S.
      Keynote Presentation: Prevalence of SARS-CoV-2 Immunity in Those...
      Learning Objectives: 1. Define SARS-CoV-2 immunity, antibody testing, and discuss what is known about the meaning of these tests 2. Identify challenges in doing research during social distan...
      Speaker: Matthew J. Memoli, MD, MS
      Presented at: Coronavirus Series
      39 1
      DEC 03, 2020 | 6:00 AM
      Keynote Presentation: Facial Masking for COVID-19: Transmission, Severity and Immunity
      Keynote Presentation: Facial Masking for COVID-19: Transmission,...
      This talk goes over the importance of face masks as a pillar of COVID-19 pandemic control. First, we review the epidemiologic evidence for how face masks reduce COVID-19 transmission from st...
      Speaker: Monica Gandhi, MD, MPH
      Presented at: Coronavirus Series
      39 1
    APR 07, 2021 | 12:00 AM
    Model-based Control for Continuous Viral Inactivation of Biopharmaceuticals
    Model-based Control for Continuous Viral Inactivation of Biopharmaceuticals
    Biologics derived from mammalian sources are expected to undergo two orthogonal virus removal processes. One common processing step is a batch low-pH hold to inactivate enveloped viruses. At...
    Speaker: Moo Sun Hong, MS
    Presented at: Bioprocessing Virtual Event Series 2021
    3 1
    APR 06, 2021 | 10:00 AM
    Invitrogen iBright Analysis Software - an introduction and overview of key analysis workflows
    Invitrogen iBright Analysis Software - an introduction and overview...
    DATE: April 6, 2021 TIME: 10:00am PDT Accurate and efficient data analysis of protein targets is an integral part of the gel and western blot imaging workflow. The Invitrogen iBright Analysi...
    Speaker: Janaki Narahari, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    1
    APR 01, 2021 | 8:00 AM
    Develop High Quality Therapeutic Antibodies Straight From Selections in Record Time
    Develop High Quality Therapeutic Antibodies Straight From Selections...
    Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
    Speaker: Andrew Bradbury, MD, PhD , Noah Ditto
    Sponsored By: Carterra
    3 1
    MAR 30, 2021 | 8:00 AM
    Utilizing the Gibcoā„¢ CTSā„¢ Roteaā„¢ System, a versatile closed manufacturing system, for cell and gene therapy development
    Utilizing the Gibcoā„¢ CTSā„¢ Roteaā„¢ System, a versatile closed manufacturing...
    DATE: March 30, 2021 TIME: 08:00am PST In this talk, we will discuss the value of moving towards modular and automated, closed-system technologies designed to enable scalable and cost-effect...
    Speaker: Mary Ann Santos , Emily Benz , Eric Dubofsky
    Sponsored By: Thermo Fisher Scientific/Gibco
    6
    MAR 25, 2021 | 8:00 AM
    Expanding ERF (Equivalent Number of Reference Fluorophore) Value Assignments for Quantitative Flow Cytometry- More Colors and Smaller Particles
    Expanding ERF (Equivalent Number of Reference Fluorophore) Value...
    Date: March 25, 2021 Time: 8:00am (PST), 11:00am (EST) Flow cytometry has expanded to have more users, more data, and multiple instrument types. While this has lead to more quantitative data...
    Speaker: Paul DeRose
    Sponsored By: Thermo Fisher Scientific
    12
     
     
  • MAR 22, 2021 | 8:00 AM
    Impact of Global Surveillance on Diagnostic Tools
    Impact of Global Surveillance on Diagnostic Tools
    Date: March 22, 2021 Time: 8:00am PDT, 11:00am EDT Viral diversity presents an ongoing challenge for diagnostic tests, which must be designed to accurately detect all current and future circ...
    Speaker: Dr. Gavin Cloherty
    Sponsored By: Scientific Affairs, Molecular Diagnostics at Abbott
    4 1
    MAR 16, 2021 | 8:00 AM
    The latest developments for enriching and analyzing antigen- and virus-specific T cells
    The latest developments for enriching and analyzing antigen- and...
    DATE: March 09, 2021 TIME: 08:00am PST The COVID-19 pandemic has placed urgency on precise analysis of rare antigen- and virus-specific T cells. At Miltenyi Biotec, we offer a complete workf...
    Speaker: Lorenz Fülle, PhD
    Sponsored By: Miltenyi Biotec
    12
    FEB 24, 2021 | 10:00 AM
    Biosafety Enclosures and the Automated Lab: Steps for Success
    Biosafety Enclosures and the Automated Lab: Steps for Success
    DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
    Speaker: David Wasescha , Simon Fogarty
    Sponsored By: Labconco
    15
    FEB 24, 2021 | 12:00 AM
    Evolution of a Platform-Based Non-Clinical Safety Assessment Approach for Vaccines
    Evolution of a Platform-Based Non-Clinical Safety Assessment Approach...
    Platform based therapeutics and vaccines offer a unique opportunity to simplify and streamline the drug development process. Moderna has taken the approach of harnessing the power of mRNA as...
    Speaker: Joseph J. Senn, PhD, DABT
    Presented at: Drug Discovery & Development Virtual Event Series 2021
    2
    FEB 11, 2021 | 8:00 AM
    Keep it small! AAV & LNP quant and sizing from just microliters
    Keep it small! AAV & LNP quant and sizing from just microliters
    Date: February 11, 2021 Time: 8:00am (PST), 11:00am (EST) Getting answers on what’s in your precious AAV, LVV, or lipid nanoparticle (LNP) sample means wanting to use the lowest possib...
    Speaker: Nelis Denys
    Sponsored By: Unchained Labs
    2
     
     
  • FEB 10, 2021 | 7:00 AM
    Influenza vaccine viral protein profiling for viral potency
    Influenza vaccine viral protein profiling for viral potency
    Date: February 10, 2021 Time: 7:00am (PST), 10:00 am (EST) In this talk today we will discuss a new approach to profile influenza proteins. Influenza vaccines typically consist of either a...
    Speaker: Stephen Lock, PhD , Patrick Buckley, PhD
    Sponsored By: SCIEX
    17
    JAN 21, 2021 | 10:00 AM
    Applications of Procalcitonin-aided Decision Making for Antibiotic Stewardship in Emergency Care
    Applications of Procalcitonin-aided Decision Making for Antibiotic...
    Date: January 21, 2021 Time: 10:00am (PST) Abstract Learning Objectives Discuss Procalcitonin as a biomarker to help distinguish viral versus bacterial infection Review the literature suppor...
    Speaker: Larissa May
    Sponsored By: Thermo Fisher Scientific
    9 1
    JAN 21, 2021 | 8:00 AM
    Critical reagent characterization for regulated bioassays accelerated by Uncle
    Critical reagent characterization for regulated bioassays accelerated...
    Date: January 21, 2021 Time: 8:00am (PST), 11:00am (EST) Today, critical reagent characterization is a key component in the overall workflow to establish robust ligand binding assays (e.g.,...
    Speaker: Ron Bowsher , Adam Kinne , Kevin Lance, Ph.D.
    Sponsored By: Unchained Labs
    6
    JAN 14, 2021 | 7:00 AM
    Cell Culture Bioprocess Scale-Up Workflow from Bench to Pilot/Production Scale using Single-Use Bioreactors
    Cell Culture Bioprocess Scale-Up Workflow from Bench to Pilot/Production...
    DATE: January 14, 2021 TIME: 7:00am PST Transferring a biologic candidate from the research and development phase to commercial production usually requires increasing the working volume...
    Speaker: Amanda Suttle , Dr. Ma Sha , Rich Mirro
    Presented at: Eppendorf Planet of Knowledge - A New Dimension
    Sponsored By: Eppendorf
    16 1
    DEC 18, 2020 | 5:00 AM
    A new approach of COVID-19 surveillance testing to maximize the identification of asymptomatic patients
    A new approach of COVID-19 surveillance testing to maximize the...
    Date: December 18, 2020 Time: 2:00pm PST Europe continues to battle a second wave of COVID19 epidemic; more than 30.000 newly infected patients are being confirmed daily in Italy. As of Nove...
    Speaker: Vittorio Sambri
    Sponsored By: Diasorin
    20 1
     
     
  • DEC 17, 2020 | 9:00 AM
    Advancing Cell Biology with Cryo-Correlative Microscopy
    Advancing Cell Biology with Cryo-Correlative Microscopy
    Date: December 17, 2020 Time: 9:00am (PST), 12:00pm (EST) Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging moda...
    Speaker: Elizabeth Wright, Ph.D. , Jae Yang, Ph.D. , Bryan Sibert, Ph.D. , Joseph Kim
    Sponsored By: Leica Microsystems, Alveole
    13 2
    DEC 15, 2020 | 10:00 AM
    Prep for Success: From human microbiome exploration to advanced SARS-CoV-2 testing in wastewater
    Prep for Success: From human microbiome exploration to advanced...
    DATE: December 15, 2020 TIME: 10:00am PST Scientists from Thermo Fisher Scientific will walk us through the world of microorganisms. They will discuss their most recent research on viruses,...
    Speaker: Sasha Vlassov, PhD , Jasti Madhu , Emily Zeringer
    Sponsored By: Thermo Fisher Scientific
    13
    DEC 04, 2020 | 9:00 AM
    IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody Cocktail - Visualizing immune response during infections
    IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody...
    Date: December 4, 2020 Time: 9:00am PST, 12:00pm EST Activation of Myeloid cells and interferon release is an early response to infection. The 3-marker combination (CD169/CD64/HLA-DR) gives...
    Speaker: Shankar Pattabhiraman
    Sponsored By: Beckman Coulter Life Sciences
    5
    DEC 03, 2020 | 4:30 PM
    An Iterative Approach to Structure-based in silico to in vivo Development of Combinatorial Anti-SARS-CoV-2 Therapy
    An Iterative Approach to Structure-based in silico to in vivo...
    This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
    Speaker: Garth Ehrlich, PhD, FAAAS
    Presented at: Coronavirus Series
    5
    DEC 03, 2020 | 3:00 PM
    Novel SARS-Cov-2 Neutralization Antibody test, performance and applications
    Novel SARS-Cov-2 Neutralization Antibody test, performance and...
    The presentation will provide a review of major emerging zoonotic virus outbreaks from Hendra virus in 1994 in Australia to SARS-CoV-2 in China 2019 and our latest findings on bat biology an...
    Speaker: Roumen Bogoev, MSc
    Presented at: Coronavirus Series
    Sponsored By: GenScript
    3
     
     
  • DEC 03, 2020 | 12:00 PM
    Advancing SARS-CoV-2 Diagnostics Through Standardization and Quantitation
    Advancing SARS-CoV-2 Diagnostics Through Standardization and Quantitation
    The COVID-19 pandemic continues to have a grip on worldwide health and global economics. Advancing molecular diagnostics is central to the diagnosis and prevention efforts surrounding the de...
    Speaker: Russell Garlick, PhD , Marc Salit, PhD
    Presented at: Coronavirus Series
    Sponsored By: LGC Seracare
    2
    DEC 03, 2020 | 9:00 AM
    New! PerkinElmerĀ® RT-PCR SARS-CoV-2 Solution Updates
    New! PerkinElmerĀ® RT-PCR SARS-CoV-2 Solution Updates
    To execute COVID-19 testing strategies effectively, labs need to procure reliable components of the workflow without interruption – from reagents to equipment and consumables at the sc...
    Speaker: Arvind Kothandaraman
    Presented at: Coronavirus Series
    Sponsored By: PerkinElmer
    3
    DEC 03, 2020 | 7:30 AM
    Keynote Presentation: Prevalence of SARS-CoV-2 Immunity in Those Undiagnosed with COVID-19 in the U.S.
    Keynote Presentation: Prevalence of SARS-CoV-2 Immunity in Those...
    Learning Objectives: 1. Define SARS-CoV-2 immunity, antibody testing, and discuss what is known about the meaning of these tests 2. Identify challenges in doing research during social distan...
    Speaker: Matthew J. Memoli, MD, MS
    Presented at: Coronavirus Series
    39 1
    DEC 03, 2020 | 6:00 AM
    Keynote Presentation: Facial Masking for COVID-19: Transmission, Severity and Immunity
    Keynote Presentation: Facial Masking for COVID-19: Transmission,...
    This talk goes over the importance of face masks as a pillar of COVID-19 pandemic control. First, we review the epidemiologic evidence for how face masks reduce COVID-19 transmission from st...
    Speaker: Monica Gandhi, MD, MPH
    Presented at: Coronavirus Series
    39 1
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