MAY 12, 2021 10:32 AM PDT

Reversing the Heart Damage Seen in Marfan Syndrome

WRITTEN BY: Carmen Leitch

Marfan syndrome is a genetic disorder that disrupts the connective tissues that anchor and link the body's organs, affecting the eyes, heart, skeleton, and blood vessels. Patients with Marfan often experience a progressive weakening in the aortic wall of the heart, called aortic aneurysm (AA). While AA is not always a problem, it can cause the aorta to rupture or dissect. This complication, called thoracic aortic aneurysm and dissection (TAAD) is also common, a lethal condition.

Sara Martínez Martínez, María José Méndez Olivares, Álvaro Alfayate, , Andrea de la Fuente-Alonso, Miguel R. Campanero, Juan Miguel Redondo y Jesús Vázquez.. / Credit: CNIC

Researchers have now found that a biochemical pathway involving nitric oxide (NO) is overactive in the aortas of both Marfan patients and a mouse model of the disease. This overactivation seems to be the cause of aortic aneurysms in Marfan patients. These findings have been reported in Nature Communications.

"Aortic dissection accounts for more than 90 percent of deaths associated with Marfan Syndrome," noted the co-director of the study, Dr. Juan Miguel Redondo of Centro Nacional de Investigaciones Cardiovasculares (CNIC).

Right now, treatments for Marfan syndrome aim to reduce the pressure blood exerts on the arterial wall, but do not stop the wall from degenerating. Surgery is the only option for patients with aortic disease. Now that NO has been identified as a crucial player in the aortic disease seen in Marfan syndrome, the NO pathway can be investigated for its potential as a biomarker or drug target.

The new study has built on other observations by this research team. "We previously detected high expression of a protein with a high capacity for NO production in the aortas of Marfan Syndrome patients and an animal model of the disease, and we therefore undertook an in-depth investigation of the role of NO in the associated aortic disease," explained study co-director Dr. Miguel Campanero of the Centro de Biología Molecular Severo Ochoa (CBM-CSIC-UAM).

Staining showing pVASP-S239 (red), elastic fibers (green) and nuclei (blue) in the aortic wall of a healthy donor (Healthy Aorta) and a patient with Marfan Syndrome (Diseased Aorta from Marfan Patient). The images show how the NO-sGC-PRKGI pathway is over-activated in the aortic wall from Marfan patiens. / Credit: CNIC/ CSIC

The scientists found that in Marfan Syndrome, high levels of NO can lead to TAAD. "We observed that treatment of healthy mice with supra-pharmacological doses of an NO donor induced TAAD similar to that seen in Marfan mice. The NO donor treatment also reproduced the degeneration of the aortic wall, an essential step in the development of TAAD," added Redondo. "Through these experiments, we showed that elevated production of NO is necessary and sufficient for the development of TAAD in Marfan Syndrome."

Once the researchers revealed the role of NO in TAAD, they investigated whether they could manipulate the pathway. They focused on two enzymes regulated by NO, soluble guanylate cyclase (sGC) and type I cGMP-dependent protein kinase (PRKG1) which are both found at abnormally high levels in Marfan patients and the mouse disease model.

"We were able to completely reverse the aortic disease in Marfan mice by treating them with inhibitors of these two proteins or by genetically silencing the expression of Prkg1, demonstrating that the NO-sGC-PRKG1 pathway mediates the development of TAAD in Marfan Syndrome," revealed Campanero.

Aortic sections show disarrayed elastic fibers (black lines), thick vascular wall in Marfan mouse, reverts when Prkg1 protein is reduced. / Credit: CNIC/CSIC

"The results of this study open the way to the use of sGC and PRKG1 inhibitors in preclinical and clinical trials for this syndrome and possibly other aortic diseases," said Redondo.

High NO levels were also linked to molecular changes in the blood that could serve as biomarkers of disease.

"Working with the CNIC Proteomics Unit and clinical groups at Vall D´Hebron, Puerta de Hierro, Marqués de Valdecilla, and Ghent University hospitals, we found that the high NO levels in Marfan Syndrome lead to increases in protein nitration and cGMP in the blood of mice and patients with this disease," said Campanero.

"This discovery has important implications for patients with this syndrome because these molecules could be used as biomarkers for disease monitoring, and we are now studying their potential as prognostic indicators," explained Redondo.

Sources: AAAS/Eurekalert! via CNIC, Nature Communications

About the Author
  • Experienced research scientist and technical expert with authorships on over 30 peer-reviewed publications, traveler to over 70 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
MAY 19, 2021
Health & Medicine
Who Ages Better, Men or Women?
MAY 19, 2021
Who Ages Better, Men or Women?
The answer depends on what's considered more important – quantity or quality of years?  Previous studies ...
JUL 13, 2021
Health & Medicine
The Science of Crying
JUL 13, 2021
The Science of Crying
Crying: In humans, it's the production of tears usually accompanied by facial muscle contractions and vocal sounds. ...
AUG 05, 2021
Clinical & Molecular DX
Wearable Patch Senses Blood Vessel Blockages
AUG 05, 2021
Wearable Patch Senses Blood Vessel Blockages
The cardiovascular system is like a network of highways, filled with an estimated 25 trillion red blood cells that conti ...
AUG 17, 2021
Health & Medicine
Heart Attack and Stroke Related to COVID19- The Importance of Prevention
AUG 17, 2021
Heart Attack and Stroke Related to COVID19- The Importance of Prevention
The historic scientific breakthrough of vaccination has prevented lifelong disability from Polio, scarring from Mea ...
OCT 09, 2021
Cardiology
Elevated Blood Pressure Linked to Decline in Brain Health
OCT 09, 2021
Elevated Blood Pressure Linked to Decline in Brain Health
New research has confirmed previous work indicating that there's a connection between elevated blood pressure and cognit ...
OCT 19, 2021
Cardiology
Can Optimism Save Lives?
OCT 19, 2021
Can Optimism Save Lives?
Throughout history, optimism has been viewed as a cornerstone of resilience. In the words of Helen Keller, optimism is t ...
Loading Comments...