APR 28, 2014 12:00 AM PDT

Muscle Weakness Linked to Damaged Mitochondria

WRITTEN BY: Jen Ellis
People suffering from mitochondrial disease and those who have an extended history of alcoholism have the common symptom of muscle weakness. A study that was recently published in The Journal of Cell Biology appears to have unearthed the reason why.

Researchers from Thomas Jefferson University in Philadelphia discovered that the common thread is mitochondria that are incapable of repairing themselves. Mitochondria provide energy for virtually every cell in the human body, thus a reduction in functioning mitochondria could certainly explain muscle weakness.

Mitochondria typically repair themselves with a fusion process - combining with nearby mitochondria and blending components. The damaged components are swapped out with healthy protein sections from normal mitochondria, removed from the repaired structure, and isolated for future use.

However, skeletal muscles are tightly packed with fibers, with mitochondria compressed within the muscle structure. It has been assumed that the repair mechanism would be different within muscle, because of the lack of space and mobility to allow fusion to take place.

The research team suspected that fusion might still be a valid mechanism within muscle mitochondria, bolstered by the recent findings on two different mitochondrial disorders. The diseases ADOA (Autosomal Dominant Optical Atrophy) and a form of CMT (Charcot-Marie-Tooth) share muscle weakness as a symptom, and patients of these diseases have a mutation in a gene affecting the fusion of mitochondria.

For testing purposes, a strain of rats was created with mitochondria that always expressed a red color. This mitochondrial variation was also designed to change to a green color upon contact with a specific wavelength of laser output, thus producing a green square of mitochondria amid the background.

The team did produce the green squares as expected with laser activation, showing the exchange of contents with nearby red mitochondria. However, the "green" mitochondria were detected migrating within a muscle structure for significant distances, indicating fusion could take place there - and fusion did indeed happen.

Once the migration process and corresponding fusion was proven to be possible in muscle cells, could it be correlated to the muscle weakness mechanisms? The next step was to determine which of the proteins played a dominant role in the fusion inside skeletal muscle cells. With further research, the team determined that a protein labeled Mfn1 was the primary protein for the fusion process.

With the target protein identified, the team then tested the effect of alcohol. Rats that were given alcohol on a regular basis suffered from lower levels of Mfn1 - as low as half the amount in some cases. Mitochondrial fusion in these rats dropped drastically as a result. Replenishing the Mfn1 supply restored the mitochondrial fusion process, and reduced the muscle fatigue exhibited by the rats.

With the ability to turn the syndrome off and on via Mfn1 levels, the team proved the link between muscle weakness and mitochondria that could not repair themselves. In practical terms, since the dominant protein was identified, this work may lead to effective drug treatments against these forms of muscle weakness.
About the Author
You May Also Like
OCT 22, 2019
Cell & Molecular Biology
OCT 22, 2019
Investigating the Molecular Basis of Sex-related Differences in Cancer
There are sex-specific differences in the rate and lethality of various cancers....
OCT 22, 2019
Genetics & Genomics
OCT 22, 2019
APOE4 Carriers Have Memory Problems Before the Onset of Alzheimer's
Changes in the genes we carry can have a big impact on our risk of getting certain diseases....
OCT 22, 2019
Immunology
OCT 22, 2019
Your Immune Response Varies from AM to PM
“My biological clock is ticking.” We’ve heard people say this phrase - maybe even said it ourselves - but what do we mean exactly? Often...
OCT 22, 2019
Microbiology
OCT 22, 2019
Using CRISPR to Alter or Kill Bacteria
In recent years, the gene editing tool CRISPR/Cas9 has been applied to a wide variety of different organisms, and now, bacteria....
OCT 22, 2019
Cell & Molecular Biology
OCT 22, 2019
Understanding How Life Could Have Arisen From RNA
Around 4 billion years ago, scientists think that life arose, and they have been trying to learn more about how that happened...
OCT 22, 2019
Genetics & Genomics
OCT 22, 2019
Membrane-less Organelle Found to Play a Role in Preventing Cancer
In recent years, scientists have found that a phenomenon called phase separation plays a critical role in the functions of cells....
Loading Comments...