FEB 04, 2019 5:11 AM PST

Insight Into the Earliest Stages of Life on Earth

WRITTEN BY: Carmen Leitch

Scientists have been looking for the origins of life on earth for many years. One theory that has emerged involves a biological molecule called RNA, which is used in cells to carry genetic information from the genome to cellular machinery that translates or uses it. New work has found that RNA molecules can be brought together inside of droplets without membranes, which then assemble as “complex coacervates.” Inside of those droplets, a concentration of RNA molecules initiates enzymatic activity, encouraging chemical reactions. This research, which helps reconstruct the possible early events in the so-called prebiotic RNA world, has been outlined in Nature Communications.

The Image shows droplets of complex coacervates as seen under a microscope. The inset shows RNA molecules (cyan) are highly concentrated inside the droplets compared to the surrounding (dark). At roughly 2-5 micrometers in diameter, the droplets are about 14-35 times thinner than human hair. / Credit: Bevilacqua Laboratory, Penn State

"We're interested in how you go from a world with no life to one with life," said one of the senior authors of the study Philip C. Bevilacqua, Distinguished Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State. "One can imagine a lot of steps in this process, but we are not looking at the most elemental steps. We are interested in a slightly later step, to see how RNA molecules could form from their basic building blocks and if those RNA molecules could drive the reactions needed for life in the absence of proteins."

Life now requires the DNA in the genome, which is transcribed into RNA to make proteins and carry out cellular functions. To create DNA, proteins are necessary, so a chicken and egg argument is set up - which came first?

"RNA, or something similar, has been thought of as a key to solving this dilemma," noted the first author of the report Raghav R. Poudyal, the Simons Origins of Life Postdoctoral Fellow at Penn State. "RNA molecules carry genetic information, but they can also function as enzymes to catalyze the chemical reactions needed for early life. This fact has led to the notion that life on Earth went through a stage where RNA played an active role in facilitating chemical reactions - "the RNA World" where self-replicating RNA molecules both carried the genetic information and performed functions that are now generally carried out by proteins."

The RNA origin of life theory is outlined in the video above by NOVA PBS.

Cells are bound and separated from one another by membranes, but such membranes would probably have been an impediment in the primordial soup. The molecules needed to be free to interact, but also had to be gathered together to have any effect.

"You can think of these RNA enzymes like a car being produced in an assembly line," explained Poudyal. "If you don't have the parts in the right place in the factory, the assembly line doesn't work. Without coacervates, the parts needed for chemical reactions are too dilute and are unlikely to find each other, but inside the coacervates, all the parts that the enzyme needs to work are nearby."

The scientists looked for materials that would have probably been around on Earth at that time and could form coacervates. Then they allowed stuff like RNA building blocks, enzymes, and their targets to engage.

"It was previously known that RNA molecules can assemble and elongate in solutions with high concentrations of magnesium," said Poudyal. "Our work shows that coacervates made from certain materials allow this non-enzymatic template-mediated RNA assembly to occur even in the absence of magnesium."

When concentrated by various charged molecules, RNA was more likely to find targets and drive chemical reactions. "These observations help us understand how the chemical environment within different membraneless compartments can impact RNA reactions,” said Christine Keating, professor of chemistry at Penn State and a senior author on the paper. 

"Although we can't look back to see the exact steps taken to form the first life on Earth, coacervates like the ones we can create in the laboratory may have helped by facilitating chemical reactions that otherwise would not have been possible," said Poudyal.

Protocells can be seen interacting in the video.


Sources: AAAS/Eurekalert! Via Penn State, Nature Communications

About the Author
  • Experienced research scientist and technical expert with authorships on over 30 peer-reviewed publications, traveler to over 70 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
DEC 17, 2020
Cardiology
A Heart Attack Induces Change in Cytokine Signaling in Patients
DEC 17, 2020
A Heart Attack Induces Change in Cytokine Signaling in Patients
Cellular signaling is critical for the body to coordinate everything from heart rate to not hitting yourself when you mo ...
DEC 17, 2020
Cell & Molecular Biology
How Water Can Prevent & Treat Obesity & Diabetes
DEC 17, 2020
How Water Can Prevent & Treat Obesity & Diabetes
Water is often recommended as a way to help people lose weight, but usually because it can help people feel full, reduci ...
DEC 27, 2020
Cell & Molecular Biology
Using Antibodies & Oligonucleotides to Control Specific Reactions
DEC 27, 2020
Using Antibodies & Oligonucleotides to Control Specific Reactions
Antibodies are naturally used by the body to bind targets on pathogens and neutralize them, and these specific interacti ...
JAN 05, 2021
Genetics & Genomics
Integrator: A New Type of Transcriptional Control is Discovered
JAN 05, 2021
Integrator: A New Type of Transcriptional Control is Discovered
The study of the genome once seemed like a straightforward process: a specific short sequence of three nucleotide bases ...
JAN 08, 2021
Cell & Molecular Biology
How Viruses Keep the Infection Going
JAN 08, 2021
How Viruses Keep the Infection Going
There has long been debate about whether viruses are a form of life, because many of them are only made up of a bit of g ...
JAN 25, 2021
Microbiology
CrAssphages and the Manipulation of the Microbiome
JAN 25, 2021
CrAssphages and the Manipulation of the Microbiome
The advancement of genetic and computational tools has given us so much new information its opened up new research field ...
Loading Comments...