JAN 04, 2017 10:47 AM PST

Scripps Florida Scientists Expand Toolbox to Study Cellular Function

OOPS! THAT EXPERIMENT FAILED...

It's not your fault! Something went wrong with our formula.
Please begin your experiment again by clicking here.

If this error continues to occur please contact us at support@labroots.com.

Credit: Oliver Burston Ikon Images Getty Images

JUPITER, FL – January 4, 2017 – Scientists on the Florida campus of The Scripps Research Institute (TSRI) have developed a new tool for studying the molecular details of protein structure.

Their new study, published recently in the journal Proceedings of the National Academy of Sciences, explores how evolution can be used to discover new and useful enzyme tools, called proteases. Proteases cleave proteins into smaller peptide pieces that scientists can then analyze to determine the identity of the protein and whether a cell has made chemical changes to the protein that might alter its function.

The new protease developed in the study helps shed light on these chemical changes, called post-translational modifications. Post-translational modifications are alterations made to proteins after the proteins are translated from RNA.

“We have to observe these protein modifications directly through chemical analysis; we can’t read them out of DNA sequence,” explained study senior author Brian M. Paegel, associate professor at TSRI.

These modifications can dramatically alter a protein’s stability and function, and unregulated modification can lead to disease, such as cancer. Therefore, understanding the nature and location of these modifications can be critical in the early phases of drug discovery.

Scientists currently rely on a technique called mass spectrometry to study post-translational modifications. With mass spectrometry, scientists analyze peptides to see if their mass changes—a bit like zooming in on that protein to see hidden details. An unexpected change in mass can indicate the occurrence of a post-translational modification.

Many scientists today use a protease called trypsin to break proteins into peptides. Because there are few other proteases available for mass spectrometry, trypsin has become the workhorse of the field. However, Paegel explained, it’s luck of the draw if trypsin generates a peptide with a modified site. So Paegel and co-workers thought it would be useful to have a new tool that cleaved directly at the modified site.

To solve this problem, Paegel developed a new trypsin “mutant” using a technique called “directed evolution.” The scientists created many thousands of trypsin mutants and tested each mutant for its ability to cut a protein at modified sites. They discovered a mutant that could cut proteins at citrulline, which is one type of modification.

Paegel believes this new approach could be useful for mapping a wider range of post-translational modifications, and he hopes to use directed evolution to discover proteases that target many other post-translational modifications. “I think we’re on the brink of an explosion of new tools for mass spectrometry,” he said.

In addition to Paegel, authors of the study, “Evolution of a mass spectrometry-grade protease with PTM-directed specificity,” were Duc. T. Tran (first author), Valerie Cavett, Vuong Q. Dang and Héctor L. Torres of TSRI.

This study was supported by a National Institutes of Health’s Director’s New Innovator Award (grant OD008535) and a National Science Foundation Research Experiences for Undergraduates Grant (1359369).

This article was originally published on Scripps.edu.

About the Author
  • The Scripps Research Institute (TSRI) is one of the world's largest independent, not-for-profit organizations focusing on research in the biomedical sciences. TSRI is internationally recognized for its contributions to science and health, including its role in laying the foundation for new treatments for cancer, rheumatoid arthritis, hemophilia, and other diseases. An institution that evolved from the Scripps Metabolic Clinic founded by philanthropist Ellen Browning Scripps in 1924, the institute now employs about 2,700 people on its campuses in La Jolla, CA, and Jupiter, FL, where its renowned scientists-including two Nobel laureates-work toward their next discoveries. The institute's graduate program, which awards PhD degrees in biology and chemistry, ranks among the top ten of its kind in the nation. For more information, see www.scripps.edu.
You May Also Like
JUN 16, 2018
Videos
JUN 16, 2018
Stem Cells can Reverse Baldness
Researchers are developing ways to treat hair loss, which is a problem for many people for a variety of reasons....
JUN 16, 2018
Cell & Molecular Biology
JUN 16, 2018
Watching the Movement of Stem Cells
Video is changing the way data is collected in the lab....
JUN 26, 2018
Health & Medicine
JUN 26, 2018
This Blood Test Can Tell if You Cheat on Your Diet
When a person ingests anything, be it food, drink or medication, it gets broken down in the bloodstream so that cells can absorb needed nutrients. When the...
JUN 27, 2018
Cell & Molecular Biology
JUN 27, 2018
Finding a new way to Stop the Growth of Cancer
Scientists discovered something new about the behavior of an organelle called ribosomes in cancer cells....
JUN 30, 2018
Cell & Molecular Biology
JUN 30, 2018
Printable Bone Grafts may Change Therapeutics
An unlimited supply of materials for transplants like bone grafts would be a game-changer....
JUL 06, 2018
Videos
JUL 06, 2018
The Impact of Junk Food on the Brain
Researchers have found that junk food stimulates neurons in our brains that cause a sense of reward....
Loading Comments...