NOV 25, 2017 4:59 PM PST

Reducing Antibiotic Use Isn't Enough to Stop Resistance

WRITTEN BY: Carmen Leitch

Antibiotic resistance is a growing health problem, one that already kills an estimated 23,000 people every year in the U.S. alone; that number is only expected to rise. There are a variety of probable causes for this problem, one of which is the overuse of antibiotics. New work has shown that bacteria are able to spread resistance capabilities among each other in a way that means that even if we reduce the use of antibiotics, bacteria will continue to gain resistance.

Escherichia coli, a bacterial strain used in this study. / Image credit: Wikimedia Commons/LenkaM

Many bacteria can easily swap genetic material through a process called conjugation. Now scientists at Duke University have found that in bacteria that are capable of conjugation, antibiotic resistance can be disseminated fast enough to move through an entire population of bacteria, even when antibiotics aren't spurring them on. The work, in which the researchers also show that this process can be disrupted, has been reported in Nature Communications

"The results came as a surprise to me when I first saw the data," said the corresponding author of the report, Lingchong You, the Paul Ruffin Scarborough Associate Professor of Engineering at Duke University. "For all of the bacteria we tested, their conjugation rate is sufficiently fast that, even if you don't use antibiotics, the resistance can be maintained, even if the genes carry a high cost."

Resistance to antibiotics may just arise by chance; it helps a bacterium survive to produce another generation, passing on the gene. That gene, however, may also carry a detrimental effect. The effect may, in turn, hinder the ability of the resistance gene to spread. For example, a mutation that allows a microbe to withstand a drug may also make it more challenging to reproduce. Such a mutation would probably disappear in time.

The balance shifts when resistance genes can merely be shared among a microbial population. If the gene spreads quickly, it promotes resistance, while the biological price of the mutation works against it.

"There have been some studies on how critical conjugation is to maintaining resistance despite its cost, but there has been a lack of careful and well-defined experiments to come to definitive conclusions," explained You. "That's where Allison has made a central contribution. Her incredibly thorough measurements allow us to draw our conclusions with high confidence."

For over a month, first author Allison Lopatkin, a doctoral candidate in the You laboratory, made careful measurements of the conjugation rate and antibiotic resistance in pathogens. 

So far, the investigators have determined that over 30 percent of the bacterial pathogens tested use conjugation to spread resistance. Lopatkin performed additional tests on several to see how well they maintained resistance when not exposed to antibiotics. "Every single clinical strain we tested maintained its resistance through conjugation even without the selective pressure of antibiotics," revealed Lopatkin.

Therefore, more efforts than just reducing antibiotic use will be required to stop the spread of antibiotic resistance. You and Lopatkin suggested we will need drugs that both stop gene swapping and slow the rate of gene dissemination through reproduction. These kinds of drugs already exist, and more may be found.

"We did the same experiments with one drug that is known to inhibit conjugation and another that encourages resistance genes to be lost," Lopatkin said. "We found that without the presence of antibiotics we could reverse the bacteria's resistance in four of the pathogens we tested and could stop it from spreading in the rest."

"As a next step, we're interested in identifying additional chemicals that can fill these roles more effectively," said You. "Historically, when researchers screened huge libraries for medicines, they focused on drugs that can kill the bacteria. But what our studies suggest is that there is a whole new universe where you can now screen for other functions, like the ability to block conjugation or to induce the loss of resistance genes. These chemicals, once proven safe, can serve as adjuvants of the standard antibiotic treatment, or they can be applied in an environmental setting as a way of generally managing of the spread of antibiotic resistance."


Sources: AAAS/Eurekalert! Via Duke University, Nature Communications

About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
JUN 03, 2020
Cell & Molecular Biology
A New Insulin That's Based on Cone Snail Venom
JUN 03, 2020
A New Insulin That's Based on Cone Snail Venom
Insulin is a hormone that's produced by a specific set of cells in the pancreas, and it functions to regulate blood ...
JUN 09, 2020
Immunology
Peacekeeping Engineered T Cells Restore the Balance in Diabetes
JUN 09, 2020
Peacekeeping Engineered T Cells Restore the Balance in Diabetes
For patients with type 1 diabetes, hope is around the corner with a new experimental therapy that uses genetically modif ...
JUL 14, 2020
Coronavirus
What Makes A Strong Antibody Response to Coronavirus
JUL 14, 2020
What Makes A Strong Antibody Response to Coronavirus
Scientists all over the world are racing to develop a vaccine that effectively and safely prompts protective immunity to ...
JUL 20, 2020
Genetics & Genomics
A Tiny But Efficient Cas Protein is Discovered in a Bacteriophage
JUL 20, 2020
A Tiny But Efficient Cas Protein is Discovered in a Bacteriophage
The microbes of the world are locked in a struggle for survival and a battle for resources. They compete directly in dif ...
JUL 21, 2020
Genetics & Genomics
In a First, DNA Quadruple Helix Observed in Live Human Cells
JUL 21, 2020
In a First, DNA Quadruple Helix Observed in Live Human Cells
If you've seen a representation of a DNA molecule, you've seen the double helix, in which two strands of genetic materia ...
AUG 12, 2020
Genetics & Genomics
A Switch That Lets Worms Toggle Between Sexes
AUG 12, 2020
A Switch That Lets Worms Toggle Between Sexes
People typically think of gender in binary terms, but in the natural world, there are many examples of sexual fluidity.
Loading Comments...