Centrifuge-Free Clarification and Harvest of AAV

This application note highlights a study that evaluates the use of Sartoclear Dynamics^® Lab Filter Aid, containing diatomaceous earth (DE), for clarifying crude lysates of adeno-associated virus (AAV) produced by HEK293 suspension cells. 

The data shows that filtration using Sartoclear Dynamics^® Lab Filter Aid is a viable clarification method for AAV2, AAV5 and AAV8. For standard AAV production processes, AAV particle recovery in the filtrate was >95%. Even difficult-to-handle serotypes such as AAV2 and lysates containing lower AAV particle titers could be clarified by Sartoclear Dynamics^® Lab filtration with viral recoveries of >80%. Moreover, the use of Sartoclear Dynamics^® Lab Filter Aid reduced manual handling time 3-fold and increased the filter capacity 3.5-fold compared to the standard laboratory method of filtration combined with a prior centrifugation step.

Learn more about the methodology in this application note.