SEP 22, 2015 03:52 PM PDT

Duke Develops Proteins That Assemble and Disassemble

Duke University scientists have figured out the genetic code that instructs proteins to either self-assemble or disassemble in response to environmental stimuli, such as changes in temperature, salinity or acidity. This provides a new platform for drug delivery systems and a completely different view of cellular functions. The study, which appears in Nature Materials, is reported in Bioscience Technology
Protein self-assemble or disassemble in response to environmental stimuli.
The advance represents the first time that scientists have reported the ability to create biological structures that are readily programmed to assemble and disassemble. Using this knowledge, researchers have opened new doors for designer proteins and investigations into nanotechnology, biotechnology and medical treatments.

Ashutosh Chilkoti, chair of the Department of Biomedical Engineering at Duke, explained, “The very simple design rules that we have discovered provide a powerful engineering tool for many biomedical and biotechnology applications. We can now, with a flick of a switch and a temperature jump, make a huge range of biological molecules that either assemble or disassemble.”

The Duke study looked at potential triggers that can cause protein structures to assemble or break apart, primarily focusing on heat. Protein-based structures that self-assemble when heated and remain stable inside of the bloodstream can be used in numerous applications. Structures exhibiting the opposite behavior have long eluded researchers, especially beyond the controlled environment of a chemistry laboratory.

As Felipe Garcia Quiroz, a former graduate student in Chilkoti’s laboratory and first author of the new study, explained, “Nobody has been able to make these kinds of materials with the degree of complexity that we have now demonstrated.”

Chilkoti’s laboratory has been designing self-assembling proteins for drug delivery systems for several years. By adding heat, these new packing materials assemble themselves and help to control where and when drugs are released inside the body through non-temperature-related mechanisms such as changes in acidity levels. The new discovery enables drugs to be encapsulated in protein cages that accumulate inside of a tumor and dissolve once heated. This would offer a more accurate way of delivering drugs, as well as a way to use the cages therapeutically.

According to Quiroz, “These packaging systems have always been inert, but now we can make these materials from bioactive components. Once the cages get there and deliver their cargo, they could break down into additional therapeutic agents. We can now design two things into one.”

The scientists say that the research also provides new insights into the everyday functions of cells. Since the laboratory identified the genetic sequences that encode this behavior, the researchers were able to point out a long list of human proteins that likely exhibit it.

“This paper shows the incredible richness of peptide sequences that already have this very simple switch,” Chilkoti concluded.
About the Author
  • Ilene Schneider is the owner of Schneider the Writer, a firm that provides communications for health care, high technology and service enterprises. Her specialties include public relations, media relations, advertising, journalistic writing, editing, grant writing and corporate creativity consulting services. Prior to starting her own business in 1985, Ilene was editor of the Cleveland edition of TV Guide, associate editor of School Product News (Penton Publishing) and senior public relations representative at Beckman Instruments, Inc. She was profiled in a book, How to Open and Operate a Home-Based Writing Business and listed in Who's Who of American Women, Who's Who in Advertising and Who's Who in Media and Communications. She was the recipient of the Women in Communications, Inc. Clarion Award in advertising. A graduate of the University of Pennsylvania, Ilene and her family have lived in Irvine, California, since 1978.
You May Also Like
AUG 26, 2018
Videos
AUG 26, 2018
All About Extremophiles
Our planet hosts some very special microbes that live in some crazy places; they are called extremophiles....
AUG 30, 2018
Cell & Molecular Biology
AUG 30, 2018
Simultaneously Assaying Gene Expression and Chromatin Availability
Traditional tools often focus on one aspect of a cell, but a new technique can assay the expression of 1000s of genes in 1000s of cells at once....
SEP 02, 2018
Cell & Molecular Biology
SEP 02, 2018
Cancer Cell Lines can Evolve in the Lab
New research shows that scientists have to take steps to verify the identity of the cell lines they grow....
SEP 14, 2018
Genetics & Genomics
SEP 14, 2018
Gout Risk Linked to Small Genetic Change
Interestingly, this genetic variation is not within the coding portion of a gene; it lies adjacent to it....
OCT 04, 2018
Genetics & Genomics
OCT 04, 2018
Similar Processes Drive the Development of Lung and Prostate Cancers
Small cell cancers often have poor prognoses....
NOV 12, 2018
Microbiology
NOV 12, 2018
Some Bacteria Gain Resistance Even Without Exposure to Antibiotics
Most bacteria are harmless, some are even beneficial to us. But some of the dangerous ones pose a real threat to public health....
Loading Comments...