SEP 05, 2016 07:51 AM PDT

Cutting Edge Technique Explains Vitamin A Transport

WRITTEN BY: Carmen Leitch
A new microscopy technique has allowed researchers at the University of Maryland School of Medicine (UM SOM) to take direct images of one of the smallest proteins ever to be seen through a microscope. The role of the protein, STRA6, is to transport Vitamin A into the interior of cells.
The STRA6 model / Credit: UM SOM

Vitamin A has roles in the production of light receptors for the eye as well as the normal development of the fetus and placenta. It is critical for mammals. For more on Vitamin A, watch the video below.

"Being able to visualize this protein, and understand how it works to move Vitamin A, is a really fantastic leap," commented one co-authors of the Science paper, David J. Weber, PhD, Professor of Biochemistry and Molecular Biology at UM SOM. "And there is so much more we can do with this technique. It's exciting."
The work was the culmination of effort by a team of researchers led by Filippo Mancia, PhD, Assistant Professor of Physiology and Cellular Biophysics at Columbia University Medical Center, and has been published in Science. An electron microscope was combined with incredible speed to capture images of the protein, which had some interesting characteristics.
"We can now get near atomic resolution because the new camera is much faster and allows us to take a movie of the molecules," explained Oliver Clarke, PhD, an associate research scientist in the lab of Wayne Hendrickson at Columbia University Medical Center. "Even under the electron microscope, the molecules are moving around by a tiny amount, but when you take a picture of something moving, it comes out blurry. With such a movie, we can align the frames of the movie to generate a sharper ima

Using about 70,000 individual pictures of STRA6, the research team was able to create a 3D map of the protein, from which a precise atomic model was made. 
The mechanism of Vitamin A transport by STRA6 was revealed by this study. It uses an intermediary protein rather than utilizing a direct interaction to move Vitamin A. By Clark’s estimation, that makes STRA6 "a bit of a freak." It may serve as a way to prevent cells from getting too much Vitamin A.
"Vitamin A is actually somewhat toxic," said another co-author, Chiara Manzini of George Washington University. "Trapping vitamin A inside the membrane may keep control of the amount inside the cell."
While additional confirmation will be required, this could be a way to explain some other mechanisms of transport.
"This collaboration among research institutions has yielded fascinating insight into the cellular pathway of vitamin A," commentedd UM SOM Dean E. Albert Reece, MD, PhD, MBA. "When scientists work together like this, great accomplishments occur. The technique developed here will clearly reap future discoveries in other domains as well."
Sources: AAAS/Eurekalert via UM SOM, Science
About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
OCT 02, 2018
OCT 02, 2018
Learning how Bacterial Chemicals Impact Human Health
We play host to a huge number of microbes, and all the chemicals that they produce....
OCT 23, 2018
OCT 23, 2018
DNA Pumps up Bacterial Cells
Cells are the basic building blocks of life, and have been well-studied since they were discovered in 1655....
OCT 24, 2018
Drug Discovery
OCT 24, 2018
Peptide Ruptures Viral Membrane of Zika Virus
According to a recent study at Nanyang Technological University, Singapore (NTU Singapore), a newly engineered antiviral peptide will aim to attack the vir...
DEC 05, 2018
DEC 05, 2018
Zika virus as a weapon against brain cancer
Glioblastoma is the most aggressive type of Brain Cancer with an average survival rate of less than two years with treatment. So what if there could be a cure for this type of cancer....
DEC 10, 2018
Health & Medicine
DEC 10, 2018
Sarcoidosis: Severe Infections and Complications
  Sarcoidosis is characterized by an exaggerated immune response to an unrecognized antigen, which has not yet been identified. It is an inflammatory...
DEC 09, 2018
DEC 09, 2018
Gut Microbiomes Vary Among Ethnicities
Many products that purport to change the microbiome have entered the market. But first we have to know what a healthy microbiome looks like....
Loading Comments...