SEP 19, 2015 5:48 PM PDT

Seeing How Two Cells Interact

WRITTEN BY: Ilene Schneider
While interactions between brain cells hold the key to healthy brain function and cognition, many of those interactions are hard to study. Researchers at the University at Buffalo and others published a paper online in Nature Communications, which was reported by Bioscience Technology, about a new method they created to accurately capture how brain cells interact.
Cell interaction holds keys to brain function and cognition.
Funded by the National Institutes of Health, the work was led by scientists at UB’s Hunter James Kelly Research Institute (HJKRI), who are attempting to better understand myelin, the fatty insulator that enables communication between nerve cells. The researchers study how damage to myelin occurs and how it can be repaired. The institute, part of UB’s New York State Center of Excellence in Bioinformatics and Life Sciences, was established in 1997 by Buffalo Bills Hall of Fame quarterback Jim Kelly and his wife Jill after their infant son Hunter, was diagnosed with Krabbe Leukodystrophy, an inherited fatal disorder. Hunter died in 2005 at the age of 8.

Cellular interactions that trigger the production of myelin are especially hard to pinpoint, because the crucial point of contact between two types of cells — the connection between axons, along which nerve impulses travel, and glial cells, which support neurons – is hidden. According to M. Laura Feltri, M.D., senior author on the paper and an HJKRI researcher and professor of biochemistry and neurology in the Jacobs School of Medicine and Biomedical Sciences at UB, “Myelin is made by a glial cell wrapping around an axon cell. To study myelin, you really need to study both cells. The glial cell wraps like a spiral around the axon, so every time you try to study the region of contact between the two cells, you end up studying the whole combination. It’s very hard to look just at the interface, and studying this interface is critical in certain diseases.”

Feltri explained, “In Krabbe’s, for example, the problem is not just that there isn’t sufficient myelin, but that the glial cell is not providing proper support to the neuron. But to figure out exactly what’s going wrong, we needed a better way to study that interface. The new technique involves using the second cell (the neuron) as a trigger to attract the first cell (the glial cell). The researchers use a system with two chambers, separated by a membrane. When the cells in the upper chamber ‘recognize’ the cells in the bottom chamber, they kind of ‘reach’ through the holes in the membrane for each other and touch. That is the intersection that we can then isolate and study.”

The researchers discovered novel proteins at the intersection called prohibitins, which are necessary for the production of myelin. The discovery could improve the understanding of and development of new treatments for myelin diseases and make it easier to study all kinds of cellular interactions, not just those in the brain.

Feltri added, “Using this method, we can isolate the portion of a cell that comes in contact with another cell, and analyze all the proteins that are present only in this subcellular fraction. It provides a glimpse into the social life of cells.This work has important implications for diseases of myelin such as Krabbe disease, and other neurodegenerative diseases, because the communication between glial cells and neurons is vital for neuroprotection.”

According to Yannick Poitelon, Ph.D., postdoctoral research scientist at HJKRI and first author of the paper, glial cells support neurons metabolically and protect axons that can measure up to one meter in length, extending far away from the glial cell. As he explained, “This has profound implications for glial disease like Krabbe’s, Charcot-Marie Tooth, peripheral neuropathies or Multiple Sclerosis, because the dysfunction of glial cells end up impairing the interactions with neurons, which, as a result, suffer and degenerate, causing devastating clinical symptoms. Similarly, neurodegenerative diseases like Huntington’s disease or Lou Gehrig's, that were considered uniquely diseases of neurons in the past, are now considered diseases of cellular communications between neurons and glial cells.”
About the Author
  • Ilene Schneider is the owner of Schneider the Writer, a firm that provides communications for health care, high technology and service enterprises. Her specialties include public relations, media relations, advertising, journalistic writing, editing, grant writing and corporate creativity consulting services. Prior to starting her own business in 1985, Ilene was editor of the Cleveland edition of TV Guide, associate editor of School Product News (Penton Publishing) and senior public relations representative at Beckman Instruments, Inc. She was profiled in a book, How to Open and Operate a Home-Based Writing Business and listed in Who's Who of American Women, Who's Who in Advertising and Who's Who in Media and Communications. She was the recipient of the Women in Communications, Inc. Clarion Award in advertising. A graduate of the University of Pennsylvania, Ilene and her family have lived in Irvine, California, since 1978.
You May Also Like
JAN 18, 2021
Cell & Molecular Biology
MicroRNAs May be Treatment Targets for Traumatic Brain Injury
JAN 18, 2021
MicroRNAs May be Treatment Targets for Traumatic Brain Injury
Traumatic brain injury, which can happen after a blow to the head, has been called a silent epidemic and is the number o ...
FEB 06, 2021
Neuroscience
Chronic Depression Linked to Reduced Support Cell Function in Brain
FEB 06, 2021
Chronic Depression Linked to Reduced Support Cell Function in Brain
Researchers from Canada have found that people with chronic depression who ultimately commit suicide have significantly ...
MAR 11, 2021
Clinical & Molecular DX
Racial Disparities in ADHD Diagnoses
MAR 11, 2021
Racial Disparities in ADHD Diagnoses
Researchers have found significant racial disparities in ADHD diagnoses and treatment: Black, Hispanic, and Asian childr ...
MAR 30, 2021
Neuroscience
Prolonged Amygdala Activity Predicts Personal Wellbeing
MAR 30, 2021
Prolonged Amygdala Activity Predicts Personal Wellbeing
Researchers have found that activity in the amygdala (the emotional center of the brain) following exposure to negative ...
APR 15, 2021
Drug Discovery & Development
Magic Mushroom Therapy and Antidepressants Produce Similar Results
APR 15, 2021
Magic Mushroom Therapy and Antidepressants Produce Similar Results
In recent years, psilocybin (the psychoactive ingredient in 'magic mushrooms') has gained increasing amounts of ...
MAY 06, 2021
Drug Discovery & Development
Tweaked Version of Ketamine Could Solve the Opioid Crisis
MAY 06, 2021
Tweaked Version of Ketamine Could Solve the Opioid Crisis
Medications that manage post-operative pain are some of the main entry points to opioid addiction and overdose in the US ...
Loading Comments...