NOV 18, 2021 9:00 AM PST

Accelerating AAV capsid purity and genome integrity analysis of adeno-associated viruses (AAV) using multi-capillary gel electrophoresis

Sponsored by: SCIEX
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract
Date:  November 18, 2021
Time: 9:00am (PDT),  12:00pm (EDT)
 
AAVs are attractive viral vectors for gene therapy due to their lack of pathogenicity, low immunogenicity, broad tropism and persistent transgene expression in both proliferating and quiescent cells. Both the purity of AAV capsid proteins and AAV genome integrity analysis are of significant importance. The purity of AAV capsid proteins can affect vector efficacy, while the analysis of AAV genome integrity provides insight into transgene integrity and ensures product safety and potency.
 
In this webinar, Tingting Li will illustrate how multi-capillary electrophoresis can be used to accelerate AAV protein purity method optimization analysis up to 12x faster than single-capillary methods, while maintaining consistent, highly sensitive results. She will also present direct comparison of genome integrity analysis on a single capillary and multi-capillary CE-LIF system. This analysis was performed, showing similar data quality and consistent corrected peak area percentage of the genome components between the two systems. With the multiplexing capability, quick assessment of the genome integrity of AAVs from different serotypes or different genome payloads can be achieved for up to 8 samples in a single run.
 
Learning Objectives
  • Discover parallel processing of eight samples simultaneously for multiple AAV serotypes
  • Achieve sensitive CE-SDS, and CIGE-LIF analysis for AAV purity and genome integrity
  • Explore seamless and easy method transfer from the single- to the multi-capillary platform
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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