WEBINAR: Addressing the Challenges of Bioconjugation for Improved Crosslinking and Modification

Speaker
  • Chief Technology Officer and Principal, Aurora Microarray Solutions, Inc. and President of Greg T. Hermanson, Inc.
    Biography
      Greg Hermanson is the chief technology officer and principal at Aurora Microarray Solutions, Inc. and the president of Greg T. Hermanson, Inc., a bioscience consulting company. Greg has over three decades of experience in the development of life science research and diagnostic products, with particular expertise in protein chemistry, assay development, immobilization, and bioconjugation. He is well known as the author of Bioconjugate Techniques, (now in its third edition), which is an extensive manual on the methods of bioconjugation. Greg has had a significant impact on the bioscience fields as proven by nearly 27,500 citations of his publications and patents.

    Abstract

    Bioconjugation is a critical technique for creating many of the key reagents used in research and commercial applications. This webinar will cover in great detail the major reactions used to form bioconjugates as well as the functional groups which react with them. This includes identifying the principle target groups on proteins, oligonucleotides, glycans, and carbohydrates and matching them with the appropriate reactive groups for conjugation. The reactive groups covered will include those useful for amine conjugations, thiol reactions, carbonyl reactions, and for bioorthogonal coupling.
     
    This webinar will examine the major products of these reactions, as well as any potential side reactions or competing hydrolysis that may occur, and any interfering substances that should be avoided. The goal of this webinar is to provide a solid understanding of these reactions and how to optimize them to create the best possible bioconjugates for the intended downstream applications.

    Learning Objectives:

    • Learn about the major reactive groups used in bioconjugation and how to optimize each reaction
    • Learn to identify major products of each reaction and understand the effects of hydrolysis, side reactions, and interfering substances
    • Discuss tips and tricks for improved results

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    WEBINAR: Addressing the Challenges of Bioconjugation for Improved Crosslinking and Modification


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