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Advanced 3D cell models for cancer biology using real time live-cell analysis

Speaker

Abstract

Integrated protocols and live-cell analysis solutions for different 3D tumor models

In the search for greater clinical translation, cancer researchers are increasingly turning to more complex live-cell assay systems that recapitulate key aspects of the tumor microenvironment. These include (1) more relevant tissue organization, (2) bio-matrices containing structural proteins and growth factors, and (3) the presence of stromal and immune cells. In this webinar, we describe integrated protocols and live-cell analysis solutions for different 3D tumor models, ranging from simple mono-culture single spheroid assays through to multi-spheroid tumor/fibroblast and tumor/immune cell co-cultures in a Matrigel biomatrix.

You will learn how to:

  • Assemble 96-well 3D spheroid assays in both round-bottom ultra-low attachment microplates, and flat plates on a Matrigel base
  • Automatically acquire and analyse images over time, using the new IncuCyte S3 DF Brightfield modality and Spheroid module
  • Quantify tumor growth, morphology and viability, either label-free or with fluorescent cell health markers
  • Study the impact of stromal cells and immune cells on tumor biology in these 3D systems
  • Gain greater biological insight and productivity in your 3D tumor assays

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Advanced 3D cell models for cancer biology using real time live-cell analysis


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