JUN 24, 2020 9:00 AM PDT

Advanced quantitative fluorescence microscopy to probe the molecular dynamics of viral entry.

Sponsored by: Leica Microsystems
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Product Application Manager, Functional Imaging, Leica Microsystems
    Biography
      Dr. Luis Alvarez studied physical chemistry at the Université Paris-Sud XI in Orsay where he worked on molecular quantitative imaging in live cells. He concentrated on fluorescence lifetime imaging and the effects of reactive oxygen species (ROS) on fluorescent proteins. In 2010, he moved to Dublin where he worked on host-pathogen interactions at University College Dublin and the National Children Research Centre. He studied how mucosal immunity uses ROS to respond to bacterial pathogen infections. During this time, he developed 3D infection models from ex-vivo organ cultures and organoids. In 2016, he moved to the University of Oxford in the UK where he developed advanced quantitative imaging approaches to study molecular mechanisms of host-viral interactions. This work centered on the understanding of HIV and EBOLA entry mechanisms into cells. He joined Leica Microsystems in 2019 as a product application manager for functional imaging.

    Abstract
    DATE:  June 24, 2020
    TIME:   9:00am PT, 12:00pm ET
     
    Some of the key factors in viral pathogenesis are the molecular mechanisms enabling viral entry into cells. Inhibitors of viral entry are often target of such mechanisms allowing to lower viral burdens during infections. Imaging experiments are specially well suited to probe the dynamic nature of viral entry. Here we will discuss how genetically-encoded biosensors paired with single virus tracking (SVT) can be used to assess the link between single cell metabolism and the corresponding ability of HIV particles to enter the cell (Coomer et al.). We will additionally see how quantitative functional imaging makes it possible to observe the time resolved stoichiometry of HIV receptor and coreceptor (CD4 and CCR5/CXCR4) during the formation of the HIV pre-fusion complex (Iliopoulou et al.). These insights into viral entry shed light on aspects of HIV entry that can be used as a basis to improve host-directed intervention strategies.
     
    Coomer CA, Carlon-Andres I, Iliopoulou M, Dustin ML, Compeer EB, Compton AA, Padilla-Parra S. Single-cell glycolytic activity regulates membrane tension and HIV-1 fusion. PLoS Pathog. 2020 Feb 21;16(2):e1008359. doi: 10.1371/journal.ppat.1008359. eCollection 2020 Feb. PMID: 32084246
     
    Iliopoulou M, Nolan R, Alvarez L, Watanabe Y, Coomer CA, Jakobsdottir GM, Bowden TA, Padilla-Parra S. A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction. Nat Struct Mol Biol. 2018 Sep;25(9):814-822. doi: 10.1038/s41594-018-0113-x. Epub 2018 Aug 27. PMID: 30150645
     
    Learning Objectives:
    • Key aspects for quantitative fluorescence imaging together with single virus tracking
    • How to multiplex biosensors to look at both viral fusion events and changes of single cell metabolism
    • How to use genetically-encoded biosensors together with single virus tracking to gain insights into molecular mechanisms of viral entry
    • How to use quantitative fluorescence imaging to decipher the time-resolved stoichiometry of membrane receptors during viral fusion
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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