Antibody Purification via the Unconventional Nucleotide Binding Site

C.E. Credits: P.A.C.E. CE Florida CE
  • Basar Bilgicer, PhD

    Professor, Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, Mike and Josie Harper Cancer Research Institute, Berthiaume Institute for Precision Health, Center for Rare & Neglected Diseases, University of Notre Dame


In search of the next generation chromatographic technique for antibody purification, our team has developed an affinity chromatography method using the unconventional nucleotide binding site (NBS). By generating resins that display ligands that bind to the NBS that is conserved on all immunoglobulins, we achieve purity over 99% with >99% column efficiency, supporting that NBS method provides a stable, reusable, and inexpensive alternative for purification of humanized and chimeric antibodies. 

Learning Objectives:

1. Describe a not-so-well-known binding site on antibody structure.

2. Describe a novel affinity purification engineering for antibodies.

3. Describe quality control measures for evaluating antibody purity.

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