OCT 03, 2019 3:00 PM PDT

'Any Idiot can do it'. How easy a knockout or knockin mouse could be generated using CRISPR/Cas9 gene editing technology?

Presented at: CRISPR 2019
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Geneticist Group leader at The John Curtin School of Medical Research (JCSMR), Australian National University
    Biography
      Dr Gaetan Burgio, is a geneticist Group leader at The John Curtin School of Medical Research (JCSMR) at the ANU. Gaetan was born in France and completed his education at Paris. He graduated in 2002 with a Medical Doctor Degree and completed his PhD in 2008 from Pasteur Institute at Paris. He migrated to Australia to join Professor Simon Foote as a postdoctoral researcher at the University of Tasmania from 2008 to 2012 and Macquarie University at Sydney from 2012 to 2015 to work on a large-scale mutagenesis in mice to uncover the role of genes that are protective against malaria infection. In 2015 he was appointed as a group leader at JCSMR to establish his research program on the development CRISPR/Cas9 gene editing technology and bacteria pathogenesis. His current research aims to explore the diversity of the CRISPR systems and to develop the technology for precision medicine or gene therapy purposes. He also leads a mouse transgenesis facility, which generated CRISPR edited mice for precision medicine.

    Abstract

    Novel precision genetic technologies such as CRISPR/Cas9 genome editing technology offer novel avenues to a better understanding the mechanisms of diseases. Using CRISPR/Cas9 we are able to precisely modify the mouse or the human genome by creating knockout or a specific single nucleotide change to enable the study of the function of the gene of interest. The generation of these models lies on the ability of Cas9 to create a double strand break in the DNA and the repair to occur via the error prone Non-Homologous End Joining (NHEJ) or the precise Homology direct Repair (HDR) mechanisms. A large body of work has been recently dedicated to either improve the technology to generate efficiently knockout or knock-in mouse models (point mutations, tags or floxed alleles). The rapid pace of the technology development has generated a lot of excitement but also some disappointment over the lack of reproducibility of the experiments. This led to a substantial loss of research time and funding. This short presentation will give an overview over the initiatives undertaken to tackle irreproducible research and we will discuss how to address these problems in the field of gene editing technology.

    Learning objectives:

    1. Generation of knock-in mouse model using CRISPR/Cas9
    2. How to design robust and reproducible experiments


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    'Any Idiot can do it'. How easy a knockout or knockin mouse could be generated using CRISPR/Cas9 gene editing technology?

    Presented at: CRISPR 2019
    C.E. Credits: P.A.C.E. CE Florida CE

    Specialty

    Molecular Biology

    Animal Research

    Immunology

    Gene Expression

    Pcr/rt-Pcr/real-Time Pcr

    Protein

    Research

    Animal Sciences

    Cancer

    Health

    T-Cells

    Dna

    Molecular Genetics

    Animal Models

    Cell Biology

    Geography

    Europe42%

    North America26%

    Asia26%

    Africa5%

    Registration Source

    Website Visitors100%

    Job Title

    Student36%

    Medical Laboratory Technician21%

    Research Scientist14%

    Scientist14%

    Medical Doctor/Specialist7%

    Educator/Faculty7%

    Organization

    Academic Institution58%

    Pharmaceutical Company11%

    Research Institute11%

    Clinical Laboratory5%

    Hospital5%

    Manufacturer - Other5%

    Other5%


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