OCT 03, 2019 9:00 AM PDT

Application of CRISPR-Cas9 in plants

Presented at: CRISPR 2019
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Post Doctoral Fellow, Global Institute for Food Security, University of Saskatchewan
    Biography
      Xingliang received his PhD in molecular biology from South China Agricultural University in Guangzhou, China. As part of his doctoral research, he constructed a set of CRISPR based gene-editing vectors for plants, and invented a degenerate sequence decoding (DSD) method for genotyping gene-edited mutants in rice and other crops. Xingliang is currently working in Tim Sharbel's Seed and Developmental Biology group in the Global Institute for Food Security at the University of Saskatchewan. His research involves studying the mechanism of apomixis with the Boechera genus, through gene-editing approach.

    Abstract

    CRISPR-Cas9 based gene editing is a powerful tool which can induce heritable mutations at desired genomic loci without leaving transgenic trace in plants. Here we constructed a robust, multiplex CRISPR/Cas9 system, including 12 intermediate sgRNA vectors and 6 binary vectors (https://www.addgene.org/Yao-Guang_Liu/), to accommodate diversified demanding in plants. We further developed an integrated CRISPR-GE web-based toolkit (http://skl.scau.edu.cn/) to fulfill bio-informatic processing and analysis requirements while using CRISPR. This CRISPR vector system has been applied in many crops including rice. As an example, we created glutinous rice with the transgene cassette segregated within two generations using our vector. This high efficiency, and user-friendly CRISPR platform are benefiting plant research and crop improvements.  

    Learning objectives:

    1. Introducing the multiplex CRISPR-Cas9 vector system
    2. CRISPR-GE web based toolkit for designing CRISPR experiment and analyzing mutants in plant

     


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