OCT 03, 2019 9:00 AM PDT

Application of CRISPR-Cas9 in plants

Presented at: CRISPR 2019
Speaker

Abstract

CRISPR-Cas9 based gene editing is a powerful tool which can induce heritable mutations at desired genomic loci without leaving transgenic trace in plants. Here we constructed a robust, multiplex CRISPR/Cas9 system, including 12 intermediate sgRNA vectors and 6 binary vectors (https://www.addgene.org/Yao-Guang_Liu/), to accommodate diversified demanding in plants. We further developed an integrated CRISPR-GE web-based toolkit (http://skl.scau.edu.cn/) to fulfill bio-informatic processing and analysis requirements while using CRISPR. This CRISPR vector system has been applied in many crops including rice. As an example, we created glutinous rice with the transgene cassette segregated within two generations using our vector. This high efficiency, and user-friendly CRISPR platform are benefiting plant research and crop improvements.  

Learning objectives:

1. Introducing the multiplex CRISPR-Cas9 vector system
2. CRISPR-GE web based toolkit for designing CRISPR experiment and analyzing mutants in plant

 


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OCT 03, 2019 9:00 AM PDT

Application of CRISPR-Cas9 in plants

Presented at: CRISPR 2019

Specialty

Gene

Molecular Genetics

Molecular Biology

Genetics

Biotechnology

Genomics

Dna

Dna Sequencing

Gene Expression

Research And Development

Gene Sequencing

Cell Biology

Biochemistry

Protein

Plant Biotechnology

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Asia48%

North America39%

Europe9%

Africa4%

Registration Source

Website Visitors100%

Job Title

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Research Scientist18%

Educator/Faculty12%

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Animal Care Lab Technician6%

Scientist6%

Organization

Academic Institution52%

Government9%

Clinical Laboratory9%

Biotech Company9%

Industrial Company4%

Diagnostic Company4%

Life Science Company4%

General Laboratory4%

Other4%


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