AUG 27, 2020 7:00 AM PDT

Atlas of the SARS-CoV-2 Proteome

Sponsored by: Sino Biological, Inc.
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • R&D Manager at Sino Biological, Inc.
    Biography
      Dr. Chen received his Ph.D. in protein biochemistry from Ohio University in 2012 and completed his post-doc training in protein biochemistry and biophysics from The Pennsylvania State University in 2016. During his academic training, Dr. Chen participated in multiple projects funded by NSF and DOE. He is highly experienced in recombinant protein expression, purification, and biochemical characterization with an outstanding track of publications in top journals. Dr. Chen currently holds the position of R&D Manager at Sino Biological, Inc. and is responsible for strategic design of recombinant protein products development and custom project oversight.

    Abstract
    DATE: August 27, 2020
    TIME: 7:00am PT, 10am ET
     
    Novel anti-viral medications, including biologics and small molecule inhibitors, as well as serologic diagnostic tool sets are in urgent demand to fight the COVID-19 pandemic. As the initial point of drug discovery, it is crucial to obtain high-quality virus proteins as starting materials for immunization and lead compound screenings. SARS-CoV-2 is comprised of a genome that yields ~25 proteins with various functionalities.
     
    This presentation focuses on our current progress of using recombinant protein expression technology to construct a protein library containing important SARS-CoV-2 structural proteins and their mutants as well as a collection of virus enzymes to facilitate drug screening campaigns. An array of spike protein mutants were expressed in eukaryote cell cultures and validated for ACE2 interactions to facilitate antibody screening and validations. Meanwhile, several optimization and engineering methods were applied to produce high quality nucleocapsid protein with correct self-assembly patterns and key SARS-CoV-2 enzymes with confirmed activities. Lastly, we introduce the continuous work-flow of a HEK293-based high-throughput antibody/protein expression platform as a powerful basis for antibody/protein library generation.

    Learning Objectives:
    • The importance of virus proteins in anti-viral drug discoveries and serologic assay tool developments
    • Development of recombinant SARS-CoV-2 protein library
    • Describe optimization and engineering methods for high-quality virus protein production
    • Discuss SARS-CoV-2 spike protein mutations
    • Introduce the high-throughput “ViteMab” recombinant antibody/protein platform

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

     


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