Real-time PCR, or quantitative qPCR, is a commonly used molecular biology lab technique to determine the actual amount of PCR product at a given cycle. For quantitative reverse transcription PCR (RT-qPCR), the starting material is RNA, which is then transcribed to cDNA by a reverse transcriptase prior to the qPCR assay. Gene expression is a common application for qPCR, and amplification through RT-qPCR is necessary to detect and quantify gene expression from small amounts of RNA. This webinar will present the basics of RT-qPCR and tips and tricks for performing RT-qPCR experiments.
1. Understand the basics of RT-PCR, including how it is used for quantitating gene expression and its advantages over other methods
2. Explore successful optimization strategies and troubleshooting tips to achieve better results