MAY 09, 2019 10:30 AM PDT

Background and Troubleshooting for RT-PCR

Sponsored by: MilliporeSigma
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Global Market Development Scientist, Gene Editing and Novel Modalities, MilliporeSigma
    Biography
      Gillian received a BSc in Biomedical Sciences, followed by a PhD in Cellular and Molecular Cancer Biology from the University of Ulster in the UK. Subsequently, she pursued almost five years of postdoctoral research in the United States utilizing a wide variety of molecular biology techniques, and co-authored 13 peer reviewed publications in the field of gene regulation and cancer biology. Her work has enabled other scientists and colleagues further research in the field of genetics, and today she serves as a strategic Global Market Development Scientist and business partner for the commercial teams as at MilliporeSigma, the Life Science business of Merck, KGaA, Darmstadt, Germany and to its gene editing customers around the world.

    Abstract

    Real-time PCR, or quantitative qPCR, is a commonly used molecular biology lab technique to determine the actual amount of PCR product at a given cycle.  For quantitative reverse transcription PCR (RT-qPCR), the starting material is RNA, which is then transcribed to cDNA by a reverse transcriptase prior to the qPCR assay. Gene expression is a common application for qPCR, and amplification through RT-qPCR is necessary to detect and quantify gene expression from small amounts of RNA. This webinar will present the basics of RT-qPCR and tips and tricks for performing RT-qPCR experiments.

    Learning Objectives: 

    1. Understand the basics of RT-PCR, including how it is used for quantitating gene expression and its advantages over other methods
    2. Explore successful optimization strategies and troubleshooting tips to achieve better results


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