MAY 09, 2019 10:30 AM PDT

Background and Troubleshooting for RT-PCR

Sponsored by: MilliporeSigma
Speaker

Abstract

Real-time PCR, or quantitative qPCR, is a commonly used molecular biology lab technique to determine the actual amount of PCR product at a given cycle.  For quantitative reverse transcription PCR (RT-qPCR), the starting material is RNA, which is then transcribed to cDNA by a reverse transcriptase prior to the qPCR assay. Gene expression is a common application for qPCR, and amplification through RT-qPCR is necessary to detect and quantify gene expression from small amounts of RNA. This webinar will present the basics of RT-qPCR and tips and tricks for performing RT-qPCR experiments.

Learning Objectives: 

1. Understand the basics of RT-PCR, including how it is used for quantitating gene expression and its advantages over other methods
2. Explore successful optimization strategies and troubleshooting tips to achieve better results


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MAY 09, 2019 10:30 AM PDT

Background and Troubleshooting for RT-PCR

Sponsored by: MilliporeSigma

Specialty

Molecular Biology

Pcr/rt-Pcr/real-Time Pcr

Molecular Diagnostics

Gene Expression

Research And Development

Molecular Genetics

Biotechnology

Clinical Diagnostics

Cancer Research

Genetics

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Dna

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Dna Sequencing

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