SEP 12, 2018 10:30 AM PDT

Bacteria that Eat Electricity: Understanding Biocathodes Using Culture Independent Techniques

Speaker
  • Research Biologist, Center for Bio/Molecular Science and Engineering, Naval Research Laboratory

Abstract

Some bacteria can use an electrode as an electron donor for respiration, generating an electrical current in the process. We are trying to understand a bacterial community that uses electricity as an energy source to fix CO2 to make biomass.  However, we are not able to grow some of the key organisms in pure cultures, so we need to use culture independent approaches. Metagenomics revealed 16 genome bins that were over 85% complete, each representing a different species. One of these species is a previously uncharacterized member of the Gammaproteobacteria that we described as “Candidatus Tenderia electrophaga.” At the genomic level, “Tenderia” has several genes also found in iron oxidizing bacteria that may allow it to make the proteins needed to transport electrons from the electrode into the cell.  It can then use them to generate the ATP and reducing equivalents necessary for CO2 fixation. We then used metatranscriptomics and metabolic inhibitors to ground truth the model developed from the “Tenderia” genome sequence. One of our lab’s goals is to develop synthetic biology systems that will help meet the needs of the US Navy. We are in the process of developing isolates from this community as chassis organisms that will be more robust in the environment that the typical lab strains used for most genetic engineering. 

Learning Objectives: 

1. Identify the ways culture independent methods are used to study difficult to culture microbes
2. Understand concept of extracellular electron transport (EET)
3. Describe the importance of reverse electron transport to some lithoautotrophic organisms


Show Resources
You May Also Like
DEC 02, 2020 8:00 AM PST
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
C.E. CREDITS
DEC 02, 2020 8:00 AM PST
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
OCT 08, 2020 7:00 AM PDT
C.E. CREDITS
OCT 08, 2020 7:00 AM PDT
DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
SEP 10, 2020 9:00 AM PDT
C.E. CREDITS
SEP 10, 2020 9:00 AM PDT
Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
AUG 25, 2020 8:00 AM PDT
C.E. CREDITS
AUG 25, 2020 8:00 AM PDT
DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
JUN 09, 2020 10:00 AM PDT
C.E. CREDITS
JUN 09, 2020 10:00 AM PDT
DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
DEC 16, 2020 8:00 AM PST
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
C.E. CREDITS
DEC 16, 2020 8:00 AM PST
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
Loading Comments...
Show Resources
Attendees
  • See more