MAY 19, 2020 1:45 PM EDT | APAC MAY 20, 2020 4:45 PM CST

Cancer Transcriptome Atlas with NGS Readout - The essential tool for spatial genomics in oncology

Speaker
  • GeoMX RNA Product Manager, NanoString Technologies
    Biography
      As a Scientist for NanoString, Kit Fuhrman has applied his understanding of immunology to solve challenging scientific problems by applying the power of the nCounter and GeoMx platforms. He developed the first 3D Flow protocols to digitally count both RNA and protein in a single tube from sorted populations. He now focuses his time building RNA applications for the Digital Spatial Profiling platform for morphology driven gene expression profiling. Kit received his Doctorate from University of Florida in immunology studying the role of regulatory T cells in type-1 diabetes pathogenesis and his Masters from the University of Central Florida directed design of HIV entry inhibitors.

    Abstract

    While many technologies focus on either exploring the spatial context of a handful of targets, or expression patterns of hundreds or thousands of targets in bulk samples or dissociated single-cells, the GeoMx Cancer Transcriptome Atlas (CTA) for use with the GeoMx DSP blends high-plex and spatial analysis in a single experiment. GeoMx DSP enables spatially resolved, digital characterization of proteins or mRNA in a highly multiplexed (>1,000-plex) assay. The assay relies upon antibody or RNA probes coupled to photocleavable oligonucleotide tags called DSP barcodes. After hybridization of probes to slide-mounted tissue sections, the DSP barcodes are released from discrete regions of the tissue via UV exposure. Released tags are quantitated via sequencing, and counts are mapped back to tissue location, yielding a spatially-resolved digital profile of analyte abundance. CTA uses the RNA probes described above and has been designed with the spatial context of cancer biology in mind, to enable studying key facets of disease without compromising sensitivity or specificity in Formalin Fixed Paraffin Embedded (FFPE) and fresh frozen tissues.


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