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JAN 14, 2021 7:00 AM PST

Cell Culture Bioprocess Scale-Up Workflow from Bench to Pilot/Production Scale using Single-Use Bioreactors

Sponsored by: Eppendorf
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Research Scientist - Eppendorf
    Biography

      Amanda Suttle works in the Applications Lab as a Research Scientist and has been a part of Eppendorf since 2014. She earned her Bachelors of Science degree in Biology from Bay Path University in 2013. Amanda has hands on experience culturing a variety of mammalian cell lines from primary T – cells to suspension CHO cells. Over the years, she has grown her expertise in scale-up applications as well as perfusion devices such as the ATF.

    • Head of Bioprocess Applications - Eppendorf
      Biography

        Dr. Ma Sha is the head of Bioprocess Applications at Eppendorf. He heads the global applications teams and laboratories in Enfield, USA and Juelich, Germany. Ma received his Ph.D. of Biochemistry from the City University of New York and conducted extensive postdoctoral research at The Rockefeller University and Harvard Medical School. Ma has over 20 years of experiences in life sciences. Prior to joining Eppendorf, Ma worked in life science companies Ciphergen Biosystems, Corning Life Sciences, and Meridian Life Sciences and held a broad variety of responsibilities such as field applications, product line management, new product development, business development, and marketing.

      • Business Manager - Eppendorf
        Biography

          Rich Mirro is a Business Manager responsible for the development of Eppendorf’s bench and pilot/production scale bioprocess solutions. Rich earned his degree in Biology from Rutgers university in 2003. He has extensive experience in upstream bioprocess applications ranging from small scale R&D up to large scale cGMP manufacturing. Rich has had various roles within Eppendorf since 2006.


        Abstract

        DATE: January 14, 2021

        TIME:  7am PST, 10 am EST, 4 pm CET

         

        Transferring a biologic candidate from the research and development phase to commercial production usually requires increasing the working volume of the upstream bioprocess. During scale-up, process performance optimized at small scale needs to be reproduced at larger scales, ideally without much need for process optimization at large working volumes. This requires reproducing the quality of the seed culture and the cells’ growth environment across scales.

        To streamline scale-up, bioengineers commonly use bioreactors with similar geometries at all scales and keep one or more parameters constant between vessels of different sizes, such as kLa, power input per volume or tip speed. Therefore, to design a scale-up strategy, data on scale-up-relevant engineering parameters need to be available for each bioreactor planned to be used. These parameters include vessel geometries, power numbers, and oxygen transfer capabilities.

        In this webinar we showcase scale-up of an antibody production process in CHO cells from bench to pilot/production scale. We discuss strategies to prepare seed cultures of reproducible quality in shake flasks. We demonstrate a new concept for software-aided scale-up strategy design, which considers vessel parameters of differently sized Eppendorf BioBLU® Single-Use Vessels and Thermo™ HyPerforma™ Single-Use Bioreactors

         

        Target group:

        •  Lab managers and research scientists interested in scaling-up their upstream cell culture bioprocess from bench to pilot/production scale
        • Lab managers and research scientists interested in single-use solutions for upstream bioprocessing

         

        Learning objectives:

        • Get to know a bioprocess workflow from shaker to pilot scale.
        • Discuss scale-up strategies used in cell culture bioprocessing
        • Learn, how the scale-up assist software feature of the new BioFlo 720 bioprocess control station can support scale-up strategy design
        • See the benefits of scalable single-use bioreactors for cell culture bioprocessing from bench to pilot/production scale

         

        This webinar is part of a virtual event. By attending the live webinar you wil  also have access to the Eppendorf Planet of Knowledge Platform on which more engaging and educational content about bioprocess scale-up will be provided.

         

        Webinars will be available for unlimited on-demand viewing after live event.

         

        LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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