MAY 04, 2016 9:00 AM PDT

Cell free methods for producing protein microarrays

Speaker
  • Interim Executive Director, Biodesign Institute Director, Biodesign Institute's Virginia G. Piper Center for Personalized Diagnostics; Virginia G. Piper Chair of Personalized Medicine; Pr
    BIOGRAPHY

Abstract
Self-assembling protein microarrays arrays can be used to study protein-protein interactions, protein-drug interactions, search for enzyme substrates, and as tools to search for disease biomarkers.  In particular, recent experiments have focused on using these protein microarrays to search for autoantibody responses in cancer patients.  These experiments show promise in finding antibody responses that appear in only cancer patients.  New methods using click chemistry-based reagents also allow the application of these arrays for discovering new substrates of post translational modification.

Objectives
  •        Learn different approaches to produce proteins for display on microarray platforms.
  •        Describe different uses of high-throughput protein display for identifying protein interactors or biomarkers.

 

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