JUN 27, 2018 8:00 AM PDT

Cell line engineering with CRISPR-Cas9 - Tips and tricks to maximize success

Sponsored by: Horizon Discovery
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Scientific Liaison and Trainer, Horizon Discovery
    Biography
      I have been using and generating cell lines and model systems for 8 years. I have worked in a number of different disciplines within biological sciences from recombinant protein expression, oncology, inflammation and cell death and apoptosis research. This is quite the jigsaw of disciplines however, one of the most consistent aspect of my work was to use gene engineering and gene modulation techniques to generate the correct model system or cell biology tool so that I could test my hypothesis and prove my scientific concept.

      I believe Genetic engineering is an inescapable technique in basic scientific research and is at the core of many biological discoveries. It is because of this initiated my industrial career in Horizon's cell engineering team and I am now sharing my gene engineering passion within Horizon's commercial team.

      It is my pleasure to share with you the years of experience the cell engineering team has so you can generate your cell model and find your genotype faster empowering your research

    Abstract
    DATE: June 27, 2018
    TIME: 08:00AM PDT
     
    CRISPR-Cas9 is a versatile tool to discover more about your biological pathway or gene of interest. The simplicity of the CRISPR-Cas9 system has led to an explosion in applications, from single cell genomics to personalized medicine.
     
     After more than 10 years of experience in cell line engineering, we have optimized all steps of the workflow. Here we present some useful tips and tricks that can help minimize the bench time needed to reach your gene-engineering goals.
     
    In this webinar, we will discuss key considerations when embarking on your gene engineering project such as designing reagents, targeting your gene of interest, and validating the resulting clones as well as the benefit of using a secondary model.
     
    Learning Objectives:
     
    • Introduction to CRISPR-Cas9 genome engineering
    • Important considerations when initiating your gene engineering projects, including:
    • Cell line optimization
    • Gene target
    • Guide choice and positioning
    • Donor design
    • Screening strategies and validation
    • Methods to rapidly determine genotype
    • Confidence in your results with thorough validation of your phenotype
     
    More resources from Horizon:
    Find out more about our ready-made Cell Lines, the ideal secondary model for your phenotype validation.

     


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