OCT 11, 2016 07:00 AM PDT

Cellular Applications for Image-based Cytometry on A Multimode Plate Reader

Speakers
  • Research Associate, Fraunhofer IME Screening Port, Hamburg
    Biography
      Maria Kuzikov studied Molecular Life Science at the University of Lübeck. She worked on the development of inhibitors targeting virus replication at the Department of Biochemistry of University of Lübeck and on assay development for studying protein-protein interactions at the European ScreeningPort. After obtaining her Master of Science degree in 2013, Maria continued to work at European ScreeningPort (now Fraunhofer IME ScreeningPort) and was responsible for development of cell-based and biochemical assays in High Throughput Screening (HTS) formats, as well as High Content Assays and early ADME-Tox profile studies. This work includes the analysis of ion channels, P450 enzymes and G-protein coupled receptors (GPCRs) as well as cell lines and primary cells throughout all stages of HTS using numerous assay formats like luminescence, IF, label-free, proximity-ligation or TR-FRET.
    • Application Specialist, PerkinElmer
      Biography
        Frauke Haenel studied Biology and achieved her PhD in 2011 at the University of Kassel, Germany. She joined PerkinElmer in May 2011 as part of the application support team based in Hamburg, Germany. The team provides support for the Multimode Detection Plate Readers to customers, sales and service. Within this team, Frauke mainly focuses on providing assistance on imaging and label-free applications, including trainings and application development.

      Abstract:
      Date: October 11th, 2016
      Time: 10:00AM EST, 4:00PM CEST, 3:00PM BST, 7:00AM PST


      Plate readers first became available about 30 years ago and have become the workhorses of research laboratories performing biochemical and cell-based assays.

      One of the newest features that has become available on multimode plate readers alongside the traditional modes such as luminescence, fluorescence and absorbance is imaging technology – the ability to generate one or more images per well of a 96- or 384-well microplate. Imaging expands the possibilities on a plate reader to ensuring assay quality and running phenotypic cell-based assays, including cell health/toxicity applications, transfection analysis, cell migration, viral infection and proliferation assays.          
       
      In this webinar, we will describe how cellular applications can benefit from fast, image-based cytometry.
       
      Who should attend
      Researchers who are interested in:
      • Cell-based assays
      • Orthogonal assays
      • Imaging and automated image analysis
       
      Key Learning Objectives
      You will learn:
      • Why cellular applications benefit from image-based cytometry
      • How image-based cytometry improves your cell-based assay data and leads to more reliable results
      • How combining imaging with other readout modes enables non-invasive monitoring of cytotoxicity parallel to the functional assay. 
      • How using imaging significantly reduces effort, cost and time when you have to optimize cell assay conditions, such as for transfection assays
      • The benefits of fast and automated image analysis on a plate reader
       
       

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