OCT 11, 2016 7:00 AM PDT

Cellular Applications for Image-based Cytometry on A Multimode Plate Reader

Speakers
  • Research Associate, Fraunhofer IME Screening Port, Hamburg
    Biography
      Maria Kuzikov studied Molecular Life Science at the University of Lübeck. She worked on the development of inhibitors targeting virus replication at the Department of Biochemistry of University of Lübeck and on assay development for studying protein-protein interactions at the European ScreeningPort. After obtaining her Master of Science degree in 2013, Maria continued to work at European ScreeningPort (now Fraunhofer IME ScreeningPort) and was responsible for development of cell-based and biochemical assays in High Throughput Screening (HTS) formats, as well as High Content Assays and early ADME-Tox profile studies. This work includes the analysis of ion channels, P450 enzymes and G-protein coupled receptors (GPCRs) as well as cell lines and primary cells throughout all stages of HTS using numerous assay formats like luminescence, IF, label-free, proximity-ligation or TR-FRET.
    • Application Specialist, PerkinElmer
      Biography
        Frauke Haenel studied Biology and achieved her PhD in 2011 at the University of Kassel, Germany. She joined PerkinElmer in May 2011 as part of the application support team based in Hamburg, Germany. The team provides support for the Multimode Detection Plate Readers to customers, sales and service. Within this team, Frauke mainly focuses on providing assistance on imaging and label-free applications, including trainings and application development.

      Abstract
      Date: October 11th, 2016
      Time: 10:00AM EST, 4:00PM CEST, 3:00PM BST, 7:00AM PST


      Plate readers first became available about 30 years ago and have become the workhorses of research laboratories performing biochemical and cell-based assays.

      One of the newest features that has become available on multimode plate readers alongside the traditional modes such as luminescence, fluorescence and absorbance is imaging technology – the ability to generate one or more images per well of a 96- or 384-well microplate. Imaging expands the possibilities on a plate reader to ensuring assay quality and running phenotypic cell-based assays, including cell health/toxicity applications, transfection analysis, cell migration, viral infection and proliferation assays.          
       
      In this webinar, we will describe how cellular applications can benefit from fast, image-based cytometry.
       
      Who should attend
      Researchers who are interested in:
      • Cell-based assays
      • Orthogonal assays
      • Imaging and automated image analysis
       
      Key Learning Objectives
      You will learn:
      • Why cellular applications benefit from image-based cytometry
      • How image-based cytometry improves your cell-based assay data and leads to more reliable results
      • How combining imaging with other readout modes enables non-invasive monitoring of cytotoxicity parallel to the functional assay. 
      • How using imaging significantly reduces effort, cost and time when you have to optimize cell assay conditions, such as for transfection assays
      • The benefits of fast and automated image analysis on a plate reader
       
       

      Show Resources
      You May Also Like
      NOV 16, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 16, 2020 8:00 AM PST
      Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
      DEC 03, 2020 9:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      DEC 03, 2020 9:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Date: December 3, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Scientific cameras used in applications such as light sheet microscopy and calcium/voltage imaging put a large emphasis on high speed...
      SEP 02, 2020 7:00 AM PDT
      C.E. CREDITS
      SEP 02, 2020 7:00 AM PDT
      DATE: September 2, 2020 TIME: 03:00pm PDT, 6:00pm EDT Spatial omics is an expanding collection of methods to examine biological molecules in their geographical context. By retaining the prec...
      AUG 18, 2020 10:00 AM PDT
      C.E. CREDITS
      AUG 18, 2020 10:00 AM PDT
      DATE: August 18, 2020 TIME: 10:00am PT Get deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the n...
      AUG 27, 2020 7:00 AM PDT
      C.E. CREDITS
      AUG 27, 2020 7:00 AM PDT
      DATE: August 27, 2020 TIME: 7:00am PT Novel anti-viral medications, including biologics and small molecule inhibitors, as well as serologic diagnostic tool sets are in urgent demand to fight...
      OCT 08, 2020 7:00 AM PDT
      C.E. CREDITS
      OCT 08, 2020 7:00 AM PDT
      DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
      Loading Comments...
      Show Resources