Challenges in tracking horizontal gene transfer of antimicrobial resistance

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Associate Professor of Medicine and Pathology, University of Virginia
    Biography

      Amy Mathers, MD, D(ABMM) is a tenured Associate Professor of Medicine and Pathology at the University of Virginia.  She acts as Clinical Director of the Adult Antimicrobial Stewardship Program and Associate Director of Clinical Microbiology for the University of Virginia Medical Center.  She has focused much of her research on the urgent clinical problem of antimicrobial resistance in Enterobacteriaceae. Molecular characterization has included analysis of mobile resistance mechanisms with evaluation of plasmid evolution and mobility across species with next generation sequencing paired with more traditional techniques.  With support from the Centers for Disease Control and Prevention she has investigated the role that the hospital plumbing environment can play in evolution and dissemination of carbapenemase genes.


    Abstract

    Focusing on the urgent clinical problem of increasing carbapenem resistance in Enterobacteriaceae we have been evaluating detection methods in clinical microbiology and molecular transmission of carbapenemase genes for the last twelve years. Molecular characterization has included analysis of mobile resistance mechanisms with evaluation of plasmid evolution and mobility across species with next generation sequencing paired with more traditional techniques.  During the talk a real world setting in which a plasmid and transposon mediated antimicrobial resistance gene transfer has occurred will act as a backdrop for understanding the different molecular approaches. We will compare and contrast the different molecular and next generation sequencing techniques for evaluating gene exchange in this context.  Through this real world example of ongoing hospital transmission involving the hospital environment and patients we can compare the advantages and disadvantages of different sequencing approaches to understand the challenges of molecular epidemiology of antimicrobial resistance transmission when horizontal gene transfer occurs.

    Learning Objectives:

    1. Understand the primary mechanisms of horizontal gene transfer between strains and species

    2. Describe why horizontal gene transfer can more quickly disseminate antimicrobial resistance

    3. Understand how long read and short read sequencing may be needed to describe the genetic context of horizontal gene transfer stting in which a plasmid mediated transmission can be seen


    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    DEC 02, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    JUL 22, 2020 10:00 AM PDT
    C.E. CREDITS
    JUL 22, 2020 10:00 AM PDT
    DATE: July 23, 2020 TIME: 10:00 am PDT The SARS-CoV-2 pandemic has taken a toll on many sectors of the medical community. As the pandemic took a grip on the laboratory, the need for diagnost...
    JUN 09, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 09, 2020 10:00 AM PDT
    DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
    AUG 25, 2020 8:00 AM PDT
    C.E. CREDITS
    AUG 25, 2020 8:00 AM PDT
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    Loading Comments...
    Show Resources