SEP 15, 2015 10:00 AM PDT

A new integrated platform for high quality and low cost gene expression analysis

Speakers
  • Founder and CEO, TATAA Biocenter
    Biography
      Dr Kubista is the founder and CEO of the TATAA Biocenter (www.tataa.com) and head of the department of gene expression at the institute of Biotechnology of the Czech Academy of Sciences. He was one of the pioneers contributing to the development of quantitative real-time PCR (qPCR) and introduced qPCR for single cell expression profiling. He led the development of reagents for high throughput single cell expression profiling and quality control at TATAA. He also developed qPCR tomography for intracellular expression profiling. Kubista coauthored the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines and he is member of the CEN and ISO workgroups drafting the forthcoming technical specifications and guidelines on the preanalytical processes in molecular diagnostics.
    • Business Development Executive, Douglas Scientific
      Biography
        Ross Higgins is a Business Development Executive with Douglas Scientific and serves in several capacities related to the development, introduction, and growing adoption of the new IntelliQube System for qPCR applications. Before joining the company, Ross worked within the clinical diagnostics field and was responsible for oversight of the molecular testing laboratory at a rapidly growing CAP-accredited startup reference laboratory. In the role, Ross and his team developed and validated new workflows and test methods that led to unprecedented throughput capacity and cost reductions. Ross has a BS in Clinical Laboratory Sciences and an MBA from Virginia Commonwealth University, and maintains an ASCP certification as a Medical Laboratory Scientist.

      Abstract
      DATE: September 15, 2015
      TIME: 10:00AM Pacific time, 1:00PM Eastern time

      The breadth of instruments available for quantitative PCR (qPCR) has continued to grow in the past 5-10 years. With older platforms now being retired and an abundance of new technologies available to replace them, lab managers, technicians, and researchers will need to effectively compare and evaluate the performance of these platforms. While new features such as multiplexing, microfluidics, and integration with liquid handling automation have enabled higher throughput and lower operating costs, it has made it increasing complex to readily compare different types of instruments and their respective performance characteristics.

      As the list of features and specifications grows, understanding some of the key metrics of instrument performance will become critical for evaluating platforms that will best meet the needs of a laboratory's application focus and assay requirements. Unfortunately, instrument vendors have not consistently conformed to any particular standards for defining and assessing performance characteristics of qPCR instruments and rarely have the methods been adequately documented in the product literature.

      In this webinar, we will define several key performance metrics of qPCR instruments such as dynamic range, Cq uniformity, sensitivity, and resolution, and further discuss their importance in practical terms. Using data from characterization and verification studies performed on the IntelliQube instrument from Douglas Scientific, we will also review approaches to evaluating these metrics, including assays and software tools that streamline the analysis and interpretation of performance testing results

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