OCT 31, 2018 8:00 AM PDT

Chromatin immunoprecipitation: Five steps to great results

Sponsored by: Thermo Fisher Scientific
Speaker
  • Scientist III, Protein Biology, Antibodies and Immunoassays, Thermo Fisher Scientific
    Biography
      Eliza has worked at Thermo Fisher Scientific since 2015. She works in Antibody and Immunoassays Group and particularly focuses on identifying new epigenetic targets to develop antibodies and antibody validation. Prior to joining Thermo Fisher Scientific, Eliza earned her PhD at The University of Chicago where she studied the mechanism of regulating the spliceosome by several pre-mRNA splicing factors. Eliza did her postdoctoral work at Northwestern University examining nucleosome position at the single cell level in yeast and the outcome of several epigenetic mutations in hematological malignancies.

    Abstract

    DATE: October 31, 2018
    TIME: 8:00am PT, 10am CT, 11:00am ET

    Chromatin immunoprecipitation (ChIP) is a technique researchers employ to obtain a snapshot of protein-DNA interactions in the cell.  ChIP provides a picture of where your protein of interest is in the genome, and understanding these interactions provides significant insight into gene expression. ChIP involves many steps and can be a challenging experiment. This webinar discusses the major steps in ChIP and provides many tips for a successful ChIP experiment, including things to consider before you start your ChIP experiment, methods for chromatin fragmentation, antibody selection, primer design, and how to analyze your qPCR data. As ChIP often needs to be tailored for cell type and protein of interest, the presenter will share tips for optimization and troubleshooting at each step.

    Learning objectives:

    • Learn how to design a successful ChIP experiment
    • Understand different methods for performing ChIP and how they can impact your experiment

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