MENU
NOV 12, 2020 6:00 AM PST

Chromogenic multiplex IHC; the future of immunohistochemistry for the practicing pathologist and for research

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Professor of Pathology Anatomy and Cell Biology, Sidney Kimmel Medical College / Thomas Jefferson University Hospital
    Biography

      Charalambos Solomides began his career in medicine at the Rheinishe Friedrich-Wilhelms University of Bonn, Germany, where he attended Medical School. Following graduation in 1988 with a Degree in Medicine he successfully completed his medical doctorate thesis in 1991, and graduated from the same university with a Doctorate of Medicine (Dr. Med.). Following 2 years in clinical research at Albany Medical College in NY, USA (1991-1993), he enrolled in the Residency Pathology Program of Temple University Hospital (TUH) in Philadelphia (1993). He completed his Residency in Pathology in 1998 and a Fellowship in Cytopathology 1999 at Thomas Jefferson University. He joined the faculty at TUH, Department of Pathology in 1999 as Assistant and later as Associate Professor of Pathology and Director of Cytopathology (2004). In 2009 he joined the Department of Pathology at Thomas Jefferson University Hospital in Philadelphia where is currently The Warren R. Lang Professor of Pathology, Anatomy and Cell Biology and Director of Cytopathology and the Immunohistochemistry Lab. His special interest is in Cytopathology and Lung Pathology. Charalambos has published over 70 publications in peer reviewed journals and 2 book chapters.


    Abstract

    The development of many different chromogens for light microscopy gives us the opportunity to explore different usage of multiplex immunohistochemistry (IHC) for clinical diagnostic purposes as well as for research. The main advantage over the immunofluorescence (IF) multiplex technology is that a special microscope or a dark room for review of the slide is not required and the pathologist can easily recognize the morphology of the specimen evaluated, giving instantly a multi-parameter semi-quantitative assessment. Also by using, image analysis and artificial intelligence (AI) algorithms, an accurate spatial and cellular distribution of the cellular components of interest can be achieved. The disadvantage is that we still have limited number of chromogens to use, and the co-expression of colors in a single cellular compartment, if more than two, is requiring image analysis algorithms.
    In this presentation you will see examples of potential clinical usage of light microscopy chromogenic multiplex IHC for diagnostic purposes. This has applications to surgical pathology as well as cytology specimens; In other words how you can replace IHC-panels of different antibodies with Multiplex IHC; or how to combine in-situ-hybridization (ISH) with protein expression on the same slide. This presentation will also demonstrate the use of Multiplex chromogenic IHC in the research setting, with examples of evaluation of tumor micro-environment with quantitative and spatial distribution of different cellular components.
    This work was performed on the Discovery BechMark Ultra and partially supported by Roche Tissue Diagnostics. The Image Analysis Algorithms were custom created for the Department of Pathology, Thomas Jefferson Universiry hospital with the VisioPharm Image Analysis software.

    Learning Objectives:
    1. The use of chromogenic multiplex IHC in research
    2. The future of chromogenic multiplex IHC in Diagnostic Cytopathology
    3. The future of chromogenic multiplex IHC in Diagnostic Surgical Pathology


    Show Resources
    You May Also Like
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    FEB 24, 2021 10:00 AM PST
    C.E. CREDITS
    FEB 24, 2021 10:00 AM PST
    DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
    MAY 11, 2021 10:00 AM PDT
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    MAR 16, 2021 10:00 AM PDT
    C.E. CREDITS
    MAR 16, 2021 10:00 AM PDT
    Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
    DEC 03, 2020 4:30 PM PST
    C.E. CREDITS
    DEC 03, 2020 4:30 PM PST
    This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
    JAN 21, 2021 8:00 AM PST
    JAN 21, 2021 8:00 AM PST
    Date: January 21, 2021 Time: 8:00am (PST), 11:00am (EST) Today, critical reagent characterization is a key component in the overall workflow to establish robust ligand binding assays (e.g.,...
    Loading Comments...
    Show Resources