To study cell metabolism, our laboratory has found it exceedingly informative to integrate Agilent Seahorse XF Analyzers with mass spectrometry-based measurements of metabolomics and stable isotope tracing to fully reveal metabolic changes in response to drug candidates. This seminar will discuss how merging quantitative, rate-based measurements of oxygen consumption with detailed pathway analysis using mass spectrometry have revealed the mitochondrial pyruvate carrier (MPC) as a central regulator of metabolic flexibility. Metabolic flexibility is the ability of cells to fuel energy metabolism and biosynthesis with multiple nutrients, and its loss is associated with disease pathogenesis in contexts as varied as type 2 diabetes, cardiac dysfunction, neurodegeneration, and certain cancers.
For Research Use Only. Not for use in diagnostic procedures.