SEP 30, 2015 06:00 AM PDT

Comparative genome and transcriptome analysis of small cell numbers

  • Director, Scientific Applications: NGS & Isothermal Amplifications, QIAGEN
      Dr. Christian Korfhage is Director, R&D Scientific Applications, at QIAGEN in Hilden. His responsibilities include the development and evaluation of new amplification and assay technologies and solutions for single cell research. Dr. Korfhage joined QIAGEN in 1997 and has held various positions in Research and Development in the areas of real-time PCR methods, isothermal amplification methods, and single cell technologies. After studying biology, he received his Ph.D. from the Institute of Genetics at the Faculty for Biology (University of Cologne, Germany) in 1993. He also completed postgraduate studies at the Max Planck Institute for Plant Breeding (Cologne) and the Institute of Molecular Neurology (Düsseldorf) in Germany.


    The genome determines changes of the transcription profile upon environmental changes and finally determines how a cell reacts. A comparative genome and transcriptome analysis of the same sample is a tool to explain changes in the transcriptome by features within genome. In order to avoid a mixed transcription profile from multiple cells, it is necessary to analyze a small cell number. For a detailed analysis of the genome and transcriptome, next-generation sequencing (NGS) is a useful method. However, it requires an amount of nucleic acid much higher than that present in a few cells. Therefore, whole genome amplification (WGA) and whole transcriptome amplification (WTA) is prerequisite for NGS. 

    Here, we describe how the genome and the transcriptome can be amplified simultaneously from a few cells in a highly parallel and comparable process. We present application data from cancer and endothelial cells. 

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