SEP 30, 2015 6:00 AM PDT

Comparative genome and transcriptome analysis of small cell numbers

Speaker

Abstract

The genome determines changes of the transcription profile upon environmental changes and finally determines how a cell reacts. A comparative genome and transcriptome analysis of the same sample is a tool to explain changes in the transcriptome by features within genome. In order to avoid a mixed transcription profile from multiple cells, it is necessary to analyze a small cell number. For a detailed analysis of the genome and transcriptome, next-generation sequencing (NGS) is a useful method. However, it requires an amount of nucleic acid much higher than that present in a few cells. Therefore, whole genome amplification (WGA) and whole transcriptome amplification (WTA) is prerequisite for NGS. 

Here, we describe how the genome and the transcriptome can be amplified simultaneously from a few cells in a highly parallel and comparable process. We present application data from cancer and endothelial cells. 


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
JUN 24, 2021 10:00 AM PDT
C.E. CREDITS
JUN 24, 2021 10:00 AM PDT
Date: June 24, 2021 Time: 10:00am PDT The Chan Zuckerberg Initiative (CZI) was founded to help solve some of society’s toughest challenges— from eradicating disease and improving...
JUN 08, 2021 9:00 AM PDT
JUN 08, 2021 9:00 AM PDT
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
SEP 30, 2015 6:00 AM PDT

Comparative genome and transcriptome analysis of small cell numbers



Show Resources
Loading Comments...
Show Resources