OCT 02, 2018 9:00 AM PDT

Comprehensive Enumeration of Immune Cells in Solid Tumors

Sponsored by: IONpath
Speaker
  • Pincipal Investigator, The Stanford Blood Center
    Biography
      Mike's academic background spans across the fields of physics, biochemistry, electrical engineering, and medicine. During his residency he became interested in developing novel methods for immunohistochemical multiplexing using mass spectrometry leading to the development of MIBI during his postdoctoral work in the Nolan lab at Stanford University. Mike is now interested in optimizing MIBI and other mass reporter-based technologies further with the goal of identifying new transcriptional and translational signatures in solid tissue malignancies, and in allergic and other immunological disorders, that can be used to improve clinical diagnosis and treatment.

    Abstract

    DATE: October 2, 2018
    TIME: 9:00AM PDT

    Understanding the role of distinct cellular phenotypes in tissue function, development, and pathogenesis requires tools that can rapidly and consistently quantify the expression of multiple proteins while preserving spatial information. Multiplexed Ion Beam Imaging by Time-Of-Flight (MIBI-TOF) detects elements from hydrogen to uranium, permitting simultaneous measurement of up to 42 labeled antibodies along with histochemical stains and native biological elements. We recently used this capability to analyze infiltrating immune cell populations in archival formalin-fixed paraffin embedded (FFPE) tissue sections from 42 triple negative breast cancer patients. Spatial enrichment analysis showed immune mixed and compartmentalized tumors, coinciding with expression of PD1, PD-L1 and IDO in a cell-type and location-specific manner. Ordered immune structures along the tumor-immune border served as a hallmark of compartmentalization and were linked to survival. We are currently working to build upon this initial effort to develop scoring mechanisms for guiding immune therapy drug selection.

    Learning Objectives:

    • Learn how MIBI technology enables simultaneous imaging of 40+ markers in FFPE tissue.
    • Learn how tumor expression and immune composition are interrelated with histological context that correlates with overall survival in TNBC

    Show Resources
    You May Also Like
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    AUG 25, 2020 8:00 AM PDT
    C.E. CREDITS
    AUG 25, 2020 8:00 AM PDT
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    OCT 02, 2018 9:00 AM PDT

    Comprehensive Enumeration of Immune Cells in Solid Tumors

    Sponsored by: IONpath

    Specialty

    Cancer Research

    Immunology

    Cancer Therapeutics

    Cancer Diagnostics

    Cell Biology

    Biotechnology

    Biomarkers

    Molecular Biology

    Immuno-Oncology

    Cell Culture

    Biochemistry

    Antibodies

    Oncology

    Clinical Research

    Molecular Diagnostics

    Geography

    North America60%

    Europe15%

    Asia12%

    Africa7%

    South America4%

    Oceania2%

    Registration Source

    Website Visitors61%

    Email Promotions22%

    WAVE Newsletter Promotion14%

    Website Banners2%

    Social Media Promotions1%

    Job Title

    Student16%

    Research Scientist15%

    Scientist14%

    Executive13%

    Educator/Faculty7%

    Medical Doctor/Specialist6%

    Medical Laboratory Technician6%

    Facility/Department Manager6%

    Post Doc5%

    Lab Management3%

    Pathologist2%

    Engineer2%

    Clinical Laboratory Scientist2%

    Biologist1%

    Marketing/Sales1%

    Organization

    Biotech Company15%

    Academic Institution15%

    Research Institute11%

    Hospital8%

    Pharmaceutical Company7%

    Medical School7%

    Clinical Laboratory7%

    Medical Center5%

    Life Science Company4%

    Government4%

    Non-Profit Organization2%

    Industrial Company2%

    Medical Device Company2%

    Private Practice1%

    Other5%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more