JUN 11, 2020 10:00 AM PDT

Correlative (Cryo-)Microscopy for Cell Biology

Sponsored by: Leica Microsystems, Alveole
C.E. Credits: P.A.C.E. CE
Speakers
  • Professor of Biochemistry; Affiliate, Morgridge Institute for Research University of Wisconsin-Madison (USA)
    Biography

      Liz received her Ph.D. in Chemistry from Emory University. She engineered elastin-mimetic materials that are used for drug delivery and tissue engineering applications. She was a postdoctoral research associate in materials science at the University of Southern California. She was a postdoctoral scholar with Professor Grant Jensen at Caltech where she developed cryo-ET technologies and used cryo-ET to study HIV-1 maturation. She joined Emory University as an Assistant Professor in 2008 and was promoted to Associate Professor in 2016. She moved to the University of Wisconsin, Madison as a full Professor in 2018. Her research program focuses on the development and use of cryo-EM and correlative light and electron microscopy (CLEM) imaging technologies to determine the native-state structures of several bacterial species, bacteriophages, HIV-1, respiratory syncytial virus (RSV), measles virus (MeV), and other host-pathogen systems.

    • Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)
      Biography

        Jae completed her Ph.D. under the mentorship of Dr. Esther Bullitt at Boston University. She studied the ultrastructure and complexity of infectious extracellular vesicles released from cells infected by non-enveloped positive-sense RNA viruses using cryo-ET and mass spectrometry. After graduation, Jae joined Dr. Wright's group at UW-Madison and has focused on development of high-precision cryo-CLEM with flexible workflows and an easy-to-use software toolkit. She also works on cryo-EM sample optimization through micro-patterning and improvement of freezing conditions for cell-virus and cell-bacteria interactions, VLPs for vaccine development, and purified viral glycoproteins.

      • Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)
        Biography

          Bryan completed his Ph.D. under the mentorship of Dr. Paul Ahlquist at UW-Madison. He studied the organization and function of viral proteins essential for assembly of positive-strand RNA virus membrane-associated RNA replication complexes. Bryan is a postdoctoral research associate in Dr. Wright's lab at the UW-Madison. His research interests center around using cryo-ET to study viral structure and assembly in multiple systems including RSV, HIV-1, and Ty1 LTR-retrotransposon.


        Abstract
        DATE:  June 11, 2020
        TIME:   10:00am PT, 1:00pm ET
         
        Correlative light and electron microscopy (CLEM) enables biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with cryo-electron tomography allows investigators to bridge knowledge gaps between cell and structural biology. In this webinar, we will review the basics of the technologies, provide examples of optimal workflows, propose equipment set-ups for research and core-facility laboratories, and provide examples of biological systems that have been studied by CLEM.
         
         
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