Are you interested in Novel Sample Prep and gaining Powerful Insights with Discovery-based LC/MS Workflows? In this session, we will demonstrate a complete untargeted metabolomics workflow from sample prep to data analysis within the context of cancer cell biology. We describe an innovative cellular metabolite extraction method that “cracks” (lyses) cells and quenches metabolism under room temperature conditions. Both polar metabolite and lipid extracts are then analyzed with the 6546 LC/Q-TOF, a powerful metabolomics platform that offers simultaneous high resolution and extended dynamic range. Finally, we leverage a full suite of recent software introductions (Lipid Annotator) and enhancements (Profinder, MPP, Quant) to elucidate the molecular response to perturbations in a cancer cell model.
For Research Use Only. Not for use in diagnostic procedures.