CRISPR revolutionized gene editing, but multi-target screening remains a complex goal. In addition, the fast pace of CRISPR technology development has brought sophisticated options for library screens including new delivery methods for CRISPR gene knockout and the possibility of genome-wide, epigenetic target gene modulation. In this talk, we will explore CRISPR screening technologies for gene knockout, activation, and repression in projects ranging in size from use of small gene panels to whole genome pools and arrayed libraries.
1. Describe general CRISPR screening considerations and workflows
2. Identify nuclease-independent applications of CRISPR