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FEB 26, 2020 9:00 AM PST

CRISPR Based Screening: Advantages of Transcriptional Activators and Inhibitors

Sponsored by: MilliporeSigma
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Senior R&D Scientist, Genome and Epigenome Editing, MilliporeSigma
    Biography
      Andrew obtained his Ph.D. in Cell Biology from Duke University, where he used mouse and zebrafish models to dissect cellular mechanisms of early tissue formation. He completed a postdoctoral fellowship in Pediatrics at the University of Colorado School of Medicine where he used pharmacological and genetic manipulations coupled with 4D imaging to investigate cell fate specification in the brain and spinal cord. Andrew joined MilliporeSigma in 2016 and has been developing novel mechanisms to modulate gene expression using CRISPR-Cas9.

    Abstract

    CRISPR revolutionized gene editing, but multi-target screening remains a complex goal.  In addition, the fast pace of CRISPR technology development has brought sophisticated options for library screens including new delivery methods for CRISPR gene knockout and the possibility of genome-wide, epigenetic target gene modulation.  In this talk, we will explore CRISPR screening technologies for gene knockout, activation, and repression in projects ranging in size from use of small gene panels to whole genome pools and arrayed libraries.

    Learning Objectives:

    1. Describe general CRISPR screening considerations and workflows

    2. Identify nuclease-independent applications of CRISPR


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