CRISPR and ZFN Mediated Gene Editing for the Exploration of Neural Cell Mechanisms and Modeling

CRISPR Cas9 nucleases have revolutionized gene editing enabling unprecedented efficiency of targeted mutagenesis.   Even with such powerful technology at hand, sophisticated projects, such as those that involve editing of neural cell types, may be challenging and time-consuming.  Similarly, due to the time and expense of exploring mechanisms of neural function neural disease modeling requires a high level of up-front design confidence.  As CRISPR becomes a focus of the molecular biology research community, MilliporeSigma seeks to share methods learned and strategies applied in our years of gene editing experience.   Today’s presentation will focus on practical Zinc Finger Nuclease and CRISPR approaches for pristine gene editing to achieve both knockout and specific sequence changes.  Special attention will be paid to publications detailing gene tagging and in vivo applications of lentiviral CRISPR reagents.