MAR 14, 2018 10:30 AM PDT

CRISPR and ZFN Mediated Gene Editing for the Exploration of Neural Cell Mechanisms and Modeling

Presented at: Neuroscience 2018
Sponsored by: MilliporeSigma
Speaker
  • Product Specialist Functional Genomics, MilliporeSigma
    Biography
      Jeremy Lehmann specializes in gRNA design, donor creation and screening assay development in his current role as a MilliporeSigma CRISPR Product Specialist. With ten years of genome editing experience, he first worked with human and mouse ES and IPS cells performing targeted integration and generating reporter cell lines and then moved into R&D in the ADME/Toxicology field developing genetically modified primary cells for use in cell based assays for the pharmaceutical industry.

    Abstract

    CRISPR Cas9 nucleases have revolutionized gene editing enabling unprecedented efficiency of targeted mutagenesis.   Even with such powerful technology at hand, sophisticated projects, such as those that involve editing of neural cell types, may be challenging and time-consuming.  Similarly, due to the time and expense of exploring mechanisms of neural function neural disease modeling requires a high level of up-front design confidence.  As CRISPR becomes a focus of the molecular biology research community, MilliporeSigma seeks to share methods learned and strategies applied in our years of gene editing experience.   Today’s presentation will focus on practical Zinc Finger Nuclease and CRISPR approaches for pristine gene editing to achieve both knockout and specific sequence changes.  Special attention will be paid to publications detailing gene tagging and in vivo applications of lentiviral CRISPR reagents.


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