MAR 14, 2018 10:30 AM PDT

CRISPR and ZFN Mediated Gene Editing for the Exploration of Neural Cell Mechanisms and Modeling

Presented at: Neuroscience 2018
Sponsored by: MilliporeSigma
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Product Specialist Functional Genomics, MilliporeSigma
    Biography
      Jeremy Lehmann specializes in gRNA design, donor creation and screening assay development in his current role as a MilliporeSigma CRISPR Product Specialist. With ten years of genome editing experience, he first worked with human and mouse ES and IPS cells performing targeted integration and generating reporter cell lines and then moved into R&D in the ADME/Toxicology field developing genetically modified primary cells for use in cell based assays for the pharmaceutical industry.

    Abstract:

    CRISPR Cas9 nucleases have revolutionized gene editing enabling unprecedented efficiency of targeted mutagenesis.   Even with such powerful technology at hand, sophisticated projects, such as those that involve editing of neural cell types, may be challenging and time-consuming.  Similarly, due to the time and expense of exploring mechanisms of neural function neural disease modeling requires a high level of up-front design confidence.  As CRISPR becomes a focus of the molecular biology research community, MilliporeSigma seeks to share methods learned and strategies applied in our years of gene editing experience.   Today’s presentation will focus on practical Zinc Finger Nuclease and CRISPR approaches for pristine gene editing to achieve both knockout and specific sequence changes.  Special attention will be paid to publications detailing gene tagging and in vivo applications of lentiviral CRISPR reagents.


    Show Resources
    You May Also Like
    SEP 05, 2019 4:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 4:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    NOV 18, 2019 7:00 AM PST
    C.E. CREDITS
    NOV 18, 2019 7:00 AM PST
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    AUG 27, 2019 9:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 9:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    MAR 24, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAR 24, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: March 24, 2020 TIME: 10:00 am PDT, 1:00pm EDT The Clampfit software module is a useful tool to manipulate and analyze electrophysiological data acquired by pCLAMP™ software. Rece...
    Loading Comments...
    Show Resources