MAR 14, 2018 10:30 AM PDT

CRISPR and ZFN Mediated Gene Editing for the Exploration of Neural Cell Mechanisms and Modeling

Presented at: Neuroscience 2018
Sponsored by: MilliporeSigma
Speaker
  • Product Specialist Functional Genomics, MilliporeSigma
    Biography
      Jeremy Lehmann specializes in gRNA design, donor creation and screening assay development in his current role as a MilliporeSigma CRISPR Product Specialist. With ten years of genome editing experience, he first worked with human and mouse ES and IPS cells performing targeted integration and generating reporter cell lines and then moved into R&D in the ADME/Toxicology field developing genetically modified primary cells for use in cell based assays for the pharmaceutical industry.

    Abstract

    CRISPR Cas9 nucleases have revolutionized gene editing enabling unprecedented efficiency of targeted mutagenesis.   Even with such powerful technology at hand, sophisticated projects, such as those that involve editing of neural cell types, may be challenging and time-consuming.  Similarly, due to the time and expense of exploring mechanisms of neural function neural disease modeling requires a high level of up-front design confidence.  As CRISPR becomes a focus of the molecular biology research community, MilliporeSigma seeks to share methods learned and strategies applied in our years of gene editing experience.   Today’s presentation will focus on practical Zinc Finger Nuclease and CRISPR approaches for pristine gene editing to achieve both knockout and specific sequence changes.  Special attention will be paid to publications detailing gene tagging and in vivo applications of lentiviral CRISPR reagents.


    Show Resources
    You May Also Like
    OCT 08, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    AUG 18, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    AUG 18, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: August 18, 2020 TIME: 10:00am PT Get deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the n...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    MAR 24, 2020 10:00 AM PDT
    C.E. CREDITS
    MAR 24, 2020 10:00 AM PDT
    DATE: March 24, 2020 TIME: 10:00 am PDT, 1:00pm EDT The Clampfit software module is a useful tool to manipulate and analyze electrophysiological data acquired by pCLAMP™ software. Rece...
    JUN 09, 2020 3:00 PM CEST
    C.E. CREDITS
    JUN 09, 2020 3:00 PM CEST
    DATE: June 9, 2020 TIME: 6am PT, 9am ET, 3pm CEST The importance of disposable plastic consumables and their overall impact on the experimental workflow of qPCR has been taken into considera...
    Loading Comments...
    Show Resources
    Attendees
    • See more