DEC 11, 2018 6:00 AM GMT

Better Data, Fewer Mice: micro-CT Imaging Breathes New Life into your Lung Research

Sponsored by: PerkinElmer
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Research Scientist, University Medical Center Göttingen
    Biography
      Dr. Christian Dullin studied technical physics at the Friedrich Schiller University Jena and finished his thesis based on extracting the 3D skull out of MRI data sets in 2001. After university he worked for three years in the research department of a small, niche company that generated patient specific skull implants. Here he was involved in developing software for extracting bone surfaces from data sets of different medical imaging techniques and for a remote consultation tool that enabled more effective interaction with clinicians. In 2004 Dr Dullin moved to the Institute for Diagnostic and Interventional Radiology at the University Medical Center Göttingen where he was responsible for technical aspects of preclinical imaging mainly working with CT and optical imaging. He finished his PhD on novel imaging strategies for preclinical lung research in 2015. During this time, he was part of several European projects, one of which was the public private partnership for asthma genomics and imaging (P3AGI). Dr Dullin has several years' experience of working with Synchrotron light sources and is currently affiliated with the Italian Synchrotron "Elettra". His work there is also predominately dedicated to lung imaging. Additionally, he has founded his own subgroup at the UMG focused on x-ray based preclinical imaging techniques. Dr. Dullin is currently the spokesperson of the x-ray study group of the European Society for Molecular Imaging.
    • Applications Scientist, PerkinElmer, Inc.
      Biography
        Dr. Sasha (Alexandre) Belenkov is an Applications Scientist at PerkinElmer, Inc. Sasha has been working in the field of preclinical imaging for more than 10 years. In his current role he is responsible for the development and support of novel imaging applications and approaches. Prior to joining PerkinElmer, he worked for VisEn Medical, GE Healthcare and ART Advanced Research Technologies. He received his B.Sc. in Anatomy and Molecular Biology and Ph.D. in Experimental Medicine at McGill University, Montreal, Canada.
      • Research Scientist, Chiesi Farmaceutici
        Biography
          Dr Francesca Ruscitti is a scientist focusing on in vivo imaging in the Pharmacology Department at Chiesi Farmaceutici in Parma, Italy. After graduating, Francesca started in the laboratory of anatomy at the Hospital of Parma investigating the role of PKC in cancer. After a period as a research fellow at Institute Marie Curie in Paris studying the immune system and HIV, she joined Chiesi company and started to work with imaging techniques as bioluminescence, fluorescence molecular tomography and computed tomography applied to pre-clinical animal models. During this time she completed her PhD in experimental respiratory physiopathology and functional diagnostics for images of the cardio-pulmonary system.

        Abstract

        DATE: December 11, 2018
        TIME: 14:00 GMT, 15:00 CET, 09:00 EST, 06:00 PST

        Animal disease models offer great value for pre-clinical research and efficient drug screening and this is also true for lung diseases such as pulmonary fibrosis, emphysema, chronic obstructive pulmonary disease (COPD), asthma, lung cancer and others. Due to the complex interaction of the immune system and the mechanical properties of the lung, animal models are an attractive option. In recent years, techniques for imaging mice (and other animals) in vivo, non-invasively, have become ubiquitous and offer the fundamental benefit of using fewer animal subjects by replacing classical histology on post mortem tissue slices. Another positive outcome is the continuity of using a single animal over time, thus yielding better data.

        One particular imaging modality, low-dose micro-computed tomography (microCT), has shown great potential for analysing pulmonary morphology and function non-invasively, longitudinally and in 3D. Imaging of pulmonary mouse models has offered some challenges in the past; the lung’s deep location, it’s highly porous nature, the fast breathing motion and size of the mouse itself, were previously limiting factors until now.

        In this webinar Dr Christian Dullin from the University Medical Centre Göttingen will explain how the PerkinElmer Quantum microCT Imaging System was used to measure alterations in lung function in mice, allowing new insights across a range of pulmonary diseases. His methodology proves that technology developments in the Quantum microCT Imaging System have overcome the previous limitations.

        Dr Sasha Belenkov of PerkinElmer will further explain the technology developments of the Quantum microCT Imaging System and how this has overcome previous challenges inherent in imaging pulmonary mouse models.

        Finally, Dr Francesca Ruscitti from Chiesi Farmaceutici in Italy will discuss how she used the Quantum microCT Imaging System in a specific lung disease study, namely fibrosis in an IPF-like bleomycin-induced murine model. Francesca will explain how microCT was used to non-invasively monitor and quantify parenchymal lesions in a murine model of lung fibrosis so they were able to follow fibrosis progression in the individual animal and thus drastically reduce the number of mice used.

        Differently from the ex vivo read outs, which require an extensive further processing of tissues of interest, microCT imaging can provide a dynamic and accurate quantification of the disease progression in longitudinal studies.

        Learning Objectives:

        • How to measure lung function using the Quantum microCT Imaging System
        • How microCT 3D imaging can reduce the number of mice used in lung disease research and improve data quality

         


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