MAR 14, 2018 12:00 PM PDT

Decoding How Clathrin is Transported to Distal Synapses Along Axons

Presented at: Neuroscience 2018
Speaker

Abstract

Clathrin mediated endocytosis (CME) at pre-synaptic terminals is functionally coupled to neuro-transmitter release. Like other cytosolic proteins, clathrin is synthesized in cell-bodies and conveyed into axons and synapses via slow axonal transport. Although the ‘triskelial’ structural conformation and endocytic function of clathrin at synapses is well-established, the cargo-structure or transport behavior of clathrin molecules moving in slow axonal transport is virtually unknown. We defined the cargo-composition and transport-kinetics of clathrin assemblies in axons using a combination of live-cell imaging, mass-spectrometry and correlative electron microscopy in primary hippocampal cultures and neurons in-vivo. We found that axonal clathrin is transported as a unique, stable cargo-complex that is distinct from the known ephemeral triskelial structures that exist at synapses and dendrites. While its transport-kinetics resembles other slow-component cargoes, surprisingly, the transport-complex contains both clathrin assembly and disassembly proteins and is ultra structurally distinct from any known clathrin assemblage. Further, we demonstrate that the stable transported clathrin cargo complex is deposited at en passant boutons along axons. Our studies thus uncover a previously-unknown form of clathrin and clarify mechanistic details of its slow transport into axons.


Show Resources
You May Also Like
JUN 28, 2022 6:45 AM PDT
JUN 28, 2022 6:45 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
APR 26, 2022 7:00 AM PDT
C.E. CREDITS
APR 26, 2022 7:00 AM PDT
Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
APR 28, 2022 8:00 AM PDT
APR 28, 2022 8:00 AM PDT
Date: April 28, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Human pluripotent stem cells (PSCs) and their derivatives hold great potentials in...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
MAR 14, 2018 12:00 PM PDT

Decoding How Clathrin is Transported to Distal Synapses Along Axons

Presented at: Neuroscience 2018


Show Resources
Loading Comments...
Show Resources