OCT 03, 2018 9:00 AM PDT

Design, Edit, Analyze and Maximize Your CRISPR-Cas9 Editing Efficiency

Presented at: CRISPR 2018
Sponsored by: Synthego
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Head of Science, Synthego
    Biography
      Kevin Holden is Head of Science at Synthego in Redwood City, California. He is part of a team responsible for integrating synthetic biology workflows, such as CRISPR genome engineering, into novel automation platforms and oversees academic and industrial collaborations with key opinion leaders in the CRISPR community. He has over 10 years of biotechnology experience that includes collaborative research in synthetic biology and genome engineering. Kevin earned his PhD in Microbiology from University of California, Davis. He is originally from the UK and immigrated to the US in his youth.

    Abstract

    Learn how to optimize CRISPR-Cas9 editing efficiencies in cell lines and primary cell types using modified synthetic sgRNA. Includes tips for achieving maximum knockout and knock-in efficiencies, experimental examples of how to optimize iPSC editing and a detailed explaination of CRISPR design and ICE analysis tools. 

    Learning Objectives: 

    1. Learn how to utilize free software tools to design CRISPR guide RNAs and analyze CRISPR editing.  
    2. Learn some key experimental parameters for optimizing CRISPR in cell lines and primary cells. 


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