Learn how to optimize CRISPR-Cas9 editing efficiencies in cell lines and primary cell types using modified synthetic sgRNA. Includes tips for achieving maximum knockout and knock-in efficiencies, experimental examples of how to optimize iPSC editing and a detailed explaination of CRISPR design and ICE analysis tools.
1. Learn how to utilize free software tools to design CRISPR guide RNAs and analyze CRISPR editing.
2. Learn some key experimental parameters for optimizing CRISPR in cell lines and primary cells.