OCT 03, 2018 09:00 AM PDT

Design, Edit, Analyze and Maximize Your CRISPR-Cas9 Editing Efficiency

Presented At CRISPR 2018
SPONSORED BY: Synthego
C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Head of Synthetic Biology, Synthego
    Biography
      Kevin Holden, PhD is Head of Synthetic Biology at Synthego in Redwood City, California. He is part of a team responsible for integrating synthetic biology workflows, such as CRISPR genome engineering, into novel automation platforms for cell engineering. In addition, he oversees collaborations with key opinion leaders in the CRISPR research community and supports commercial activities by coordinating technical field application support for Synthego's portfolio of genome engineering solutions. Kevin frequently represents Synthego at academic and industry conferences, presenting the company's latest research and development efforts.

      Kevin has over 12 years of biotechnology experience that includes collaborative research and development in synthetic biology and genome engineering. He earned his PhD in Microbiology with a Designated Emphasis in Biotechnology from The University of California, Davis. He is originally from the United Kingdom and immigrated to the United States in his youth.

    Abstract:

    Learn how to optimize CRISPR-Cas9 editing efficiencies in cell lines and primary cell types using modified synthetic sgRNA. Includes tips for achieving maximum knockout and knock-in efficiencies, experimental examples of how to optimize iPSC editing and a detailed explaination of CRISPR design and ICE analysis tools. 

    Learning Objectives: 

    1. Learn how to utilize free software tools to design CRISPR guide RNAs and analyze CRISPR editing.  
    2. Learn some key experimental parameters for optimizing CRISPR in cell lines and primary cells. 


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