NOV 05, 2019 7:00 AM PST

Detecting low-abundance proteins in a western blot: Overcoming challenges for improved signal-to-noise of low-expressing and precious sample targets

Speaker
  • Product Manager, Protein and Cell Analysis, Thermo Fisher Scientific
    Biography
      Emily is the product manager for protein detection reagents in the Biosciences Division at Thermo Fisher Scientific. She has been with the company for 5 years, coming from a product management career in the health care industry and an academic background in the biological sciences. She currently manages a product portfolio for protein detection that includes western blotting reagents and kits, protein quantitation assays, and assay development reagents. In addition to product management, Emily dedicates much of her time to interacting with customers across the academic, industrial, and diagnostic markets and working with R&D teams on new product introductions.

    Abstract

    DATE: November 5, 2019 

    TIME:  7:00am PST, 9:00am CST

    Detection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing western blotting. The abundance of the target may be low because the protein itself is expressed at low levels within the source or is difficult to extract (and thus recovery is low), the sample is limited (leaving a scarce overall volume available to load onto the gel), or because of a combination of multiple conditions. The outcome in such situations is often faint or undetectable band(s) during the imaging step, resulting in inconclusive data analysis. This can lead researchers to repeat the western blot and/or reoptimize the conditions, both of which waste time and resources. In this webinar, we will discuss specific methods from sample preparation through immunodetection that can help overcome challenges and improve signal to noise of low-abundance proteins for more successful western blot detection.

     

    Learning Objectives:

    • Identify tips and tricks for maximizing the recovery and downstream detection of target proteins from precious samples.

    • Describe specific methods to enhance signal when performing western blotting on low-abundance proteins.

     

    Webinars will be available for unlimited on-demand viewing after live event.


    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


    Show Resources
    You May Also Like
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    OCT 08, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    JUN 23, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    AUG 27, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    AUG 27, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: August 27, 2020 TIME: 7:00am PT Novel anti-viral medications, including biologics and small molecule inhibitors, as well as serologic diagnostic tool sets are in urgent demand to fight...
    Loading Comments...
    Show Resources
    Attendees
    • See more