NOV 05, 2019 7:00 AM PST

Detecting low-abundance proteins in a western blot: Overcoming challenges for improved signal-to-noise of low-expressing and precious sample targets

Speakers
  • Product Manager, Protein and Cell Analysis, Thermo Fisher Scientific
    Biography
      Emily is the product manager for protein detection reagents in the Biosciences Division at Thermo Fisher Scientific. She has been with the company for 5 years, coming from a product management career in the health care industry and an academic background in the biological sciences. She currently manages a product portfolio for protein detection that includes western blotting reagents and kits, protein quantitation assays, and assay development reagents. In addition to product management, Emily dedicates much of her time to interacting with customers across the academic, industrial, and diagnostic markets and working with R&D teams on new product introductions.

    Abstract:

    DATE: November 5, 2019 

    TIME:  7:00am PST, 9:00am CST

    Detection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing western blotting. The abundance of the target may be low because the protein itself is expressed at low levels within the source or is difficult to extract (and thus recovery is low), the sample is limited (leaving a scarce overall volume available to load onto the gel), or because of a combination of multiple conditions. The outcome in such situations is often faint or undetectable band(s) during the imaging step, resulting in inconclusive data analysis. This can lead researchers to repeat the western blot and/or reoptimize the conditions, both of which waste time and resources. In this webinar, we will discuss specific methods from sample preparation through immunodetection that can help overcome challenges and improve signal to noise of low-abundance proteins for more successful western blot detection.

     

    Learning Objectives:

    • Identify tips and tricks for maximizing the recovery and downstream detection of target proteins from precious samples.

    • Describe specific methods to enhance signal when performing western blotting on low-abundance proteins.

     

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