NOV 05, 2019 7:00 AM PST

Detecting low-abundance proteins in a western blot: Overcoming challenges for improved signal-to-noise of low-expressing and precious sample targets

Speaker

Abstract

DATE: November 5, 2019 

TIME:  7:00am PST, 9:00am CST

Detection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing western blotting. The abundance of the target may be low because the protein itself is expressed at low levels within the source or is difficult to extract (and thus recovery is low), the sample is limited (leaving a scarce overall volume available to load onto the gel), or because of a combination of multiple conditions. The outcome in such situations is often faint or undetectable band(s) during the imaging step, resulting in inconclusive data analysis. This can lead researchers to repeat the western blot and/or reoptimize the conditions, both of which waste time and resources. In this webinar, we will discuss specific methods from sample preparation through immunodetection that can help overcome challenges and improve signal to noise of low-abundance proteins for more successful western blot detection.

 

Learning Objectives:

  • Identify tips and tricks for maximizing the recovery and downstream detection of target proteins from precious samples.

  • Describe specific methods to enhance signal when performing western blotting on low-abundance proteins.

 

Webinars will be available for unlimited on-demand viewing after live event.


LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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NOV 05, 2019 7:00 AM PST

Detecting low-abundance proteins in a western blot: Overcoming challenges for improved signal-to-noise of low-expressing and precious sample targets


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